cDNA Clone CLK3

• Plasmid: pGEX6P-2-CLK3
• Parent Plasmid: pGEX6P2
• Expressed: GST-CLK3
• Genbank: NM_003992.4 GI:194097434
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTA
CCTGAGCTACCGATGGAAGAGGAGGAGGTCCTACAGTCGGGAACATGAAG
GGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATCTCGG
TCCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCG
TGACAGCGATACATACCGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGG
ACTACTATGGACCTTCACGTTCTCGTCATCGTCGGCGATCGCGGGAGAGG
GGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCAC
CAGGTCTTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCA
GCAGCCGGAGTGTGGAAGATGACAAGGAGGGTCACCTGGTGTGCCGGATC
GGCGATTGGCTCCAAGAGCGATATGAGATTGTGGGGAACCTGGGTGAAGG
CACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCTC
AGGTTGCCCTGAAGATCATCCGCAACGTGGGCAAGTACCGGGAGGCTGCC
CGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAGAAGGACAAAGAAAA
CAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACA
TGTGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAG
GAGAATAACTTCCAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTA
CCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACCCATA
CAGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACC
CTCTACAATGAGCACAAGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAG
CATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGACCATGAGCACCACA
CCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAG
CTGGGCTGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTT
TGAGTACTACCGGGGCTTCACACTCTTCCAGACCCACGAAAACCGAGAGC
ACCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACATGATC
CACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGGGA
TGAGAACAGCTCTGACGGCCGGTATGTGAAGGAGAACTGCAAACCTCTGA
AGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGCAGCTGTTTGACCTG
ATGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGA
GGCCCTGCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCT
TCCACACCAGCCGCAACCCAAGCAGATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMHHCKRYRSPEPDPYLSYR
WKRRRSYSREHEGRLRYPSRREPPPRRSRSRSHDRLPYQRRYRERRDSDT
YRCEERSPSFGEDYYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRTRSCS
SASSRSQQSSKRSSRSVEDDKEGHLVCRIGDWLQERYEIVGNLGEGTFGK
VVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLC
VLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCH
ALRFLHENQLTHTDLKPENILFVNSEFETLYNEHKSCEEKSVKNTSIRVA
DFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYR
GFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWDENSS
DGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLH
PFFAGLTPEERSFHTSRNPSR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31449
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein: 85411 Daltons