cDNA Clone PCM1

• Synonyms: *
• Plasmid: pcDNA5-FRT/TO-GFP PCM1 ISOFORM2
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-PCM1
• Genbank: NM_001315507.2
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCCACAGGAGGAGGTCCCTTTGAAGATGGCATGAATGATCA
GGATTTACCAAACTGGAGTAATGAGAATGTTGATGACAGGCTCAACAATA
TGGATTGGGGTGCCCAACAGAAGAAAGCAAATAGATCATCAGAAAAGAAT
AAGAAAAAGTTTGGTGTAGAAAGTGATAAAAGAGTAACCAATGATATTTC
TCCGGAGTCGTCACCAGGAGTTGGAAGGCGAAGAACAAAGACTCCACATA
CGTTCCCACACAGTAGATACATGAGTCAGATGTCTGTCCCAGAGCAGGCA
GAATTAGAGAAACTGAAACAGCGGATAAACTTCAGTGATTTAGATCAGAG
AAGCATTGGAAGTGATTCCCAAGGTAGAGCAACAGCTGCTAACAACAAAC
GTCAGCTTAGTGAAAACCGAAAGCCCTTCAACTTTTTGCCTATGCAGATT
AATACTAACAAGAGCAAAGATGCATCTACAAGTCCCCCAAACAGAGAAAC
GATTGGATCAGCACAGTGTAAAGAGTTGTTTGCTTCTGCTTTAAGTAATG
ACCTCTTGCAAAACTGTCAGGTGTCTGAAGAAGATGGGAGGGGAGAACCT
GCAATGGAGAGCAGCCAGATTGTAAGCAGGCTTGTTCAAATTCGCGATTA
TATTACTAAAGCTAGTTCCATGCGGGAAGATCTTGTAGAGAAAAATGAGA
GATCTGCTAATGTTGAGCGCCTTACTCATCTAATAGATCACCTTAAAGAA
CAAGAGAAGTCATATATGAAATTTCTTAAAAAAATCCTTGCCAGAGATCC
TCAGCAGGAGCCTATGGAAGAGATAGAAAATTTGAAGAAACAACATGATT
TATTAAAAAGAATGTTACAACAGCAGGAGCAACTAAGAGCTCTACAGGGA
CGGCAGGCTGCACTTCTAGCTCTGCAACATAAAGCAGAGCAAGCTATTGC
AGTGATGGATGATTCTGTTGTTGCAGAAACTGCAGGTAGCTTATCTGGCG
TCAGTATCACATCTGAACTAAATGAAGAATTGAATGACTTAATTCAGCGT
TTTCATAATCAGCTTCGTGATTCTCAGCCTCCAGCTGTTCCAGACAATAG
AAGACAGGCAGAAAGTCTTTCATTAACTAGGGAGGTTTCCCAGAGCAGGA
AACCATCAGCTTCAGAACGTTTACCTGATGAGAAAGTCGAACTTTTTAGC
AAAATGAGAGTGCTACAGGAAAAGAAACAAAAAATGGACAAATTGCTTGG
AGAACTTCATACACTTCGAGATCAGCATCTTAACAATTCATCATCCTCTC
CACAAAGGAGTGTCGATCAGAGAAGTACTTCAGCTCCCTCTGCTTCTGTA
GGCTTGGCACCGGTTGTCAATGGAGAATCCAATAGCCTCACATCATCTGT
TCCTTATCCTACTGCTTCTCTAGTATCTCAGAATGAGAGTGAAAACGAAG
GCCACCTCAATCCATCTGAAAAACTCCAGAAGTTAAATGAAGTTCGAAAG
AGATTGAATGAGCTAAGAGAATTAGTTCATTATTATGAACAAACGTCAGA
CATGATGACAGATGCTGTGAATGAAAACAGGAAAGATGAAGAAACTGAAG
AGTCAGAATATGATTCTGAGCATGAAAATTCCGAGCCTGTTACTAACATT
CGAAATCCACAAGTAGCTTCCACTTGGAATGAAGTAAATAGTCATAGTAA
TGCACAGTGTGTTTCTAATAATAGAGATGGGCGAACAGTTAATTCTAATT
GTGAAATTAACAACAGATCTGCTGCCAACATAAGGGCTCTAAACGTGCCT
CCTTCTTTAGATTGTCGATATAATAGAGAAGGGGAACAGGAGATTCATGT
TGCACAAGGTGAAGATGATGAGGAGGAGGAGGAAGAAGCAGAAGAGGAGG
GAGTCAGTGGAGCTTCATTATCTAGTCACAGGAGCAGTCTGGTTGATGAG
CATCCAGAAGATGCTGAATTTGAACAGAAGATCAACCGACTTATGGCTGC
AAAACAGAAACTTAGACAGTTACAAGATCTTGTTGCTATGGTACAGGATG
ATGATGCAGCTCAAGGAGTTATCTCTGCCAGTGCATCAAATTTGGATGAT
TTCTACCCAGCAGAAGAAGACACCAAGCAAAATTCAAATAACACTAGAGG
AAATGCCAATAAAACACAGAAAGATACTGGAGTAAATGAAAAGGCAAGAG
AGAAATTTTATGAGGCTAAACTACAGCAGCAACAGAGAGAGCTAAAACAA
TTGCAGGAAGAAAGAAAGAAACTGATTGACATTCAGGAGAAAATTCAAGC
ATTGCAAACGGCATGCCCTGACTTACAGCTGTCAGCTGCTAGTGTGGGTA
ACTGTCCTACCAAAAAATATATGCCAGCTGTTACTTCAACCCCAACTGTT
AATCAACACGAGACCAGTACAAGCAAATCTGTTTTTGAGCCTGAAGATTC
TTCAATAGTAGATAATGAGTTGTGGTCAGAAATGAGAAGACATGAAATGT
TGAGGGAGGAGCTGCGACAGAGAAGAAAGCAGCTTGAAGCTCTGATGGCT
GAACATCAGAGGAGGCAAGGTCTAGCTGAAACTGCATCTCCAGTGGCTGT
GTCATTGAGAAGTGATGGATCTGAGAACCTATGTACTCCTCAGCAAAGTA
GAACAGAAAAAACGATGGCAACTTGGGGAGGGTCTACCCAGTGTGCACTA
GATGAAGAAGGAGATGAAGACGGTTACCTTTCTGAAGGAATTGTTCGGAC
AGATGAAGAGGAGGAAGAAGAGCAAGATGCCAGTTCCAATGATAACTTTT
CTGTGTGTCCTTCTAACAGTGTGAATCATAACTCCTACAATGGAAAGGAA
ACTAAAAATAGGTGGAAGAACAATTGCCCTTTTTCGGCAGATGAAAATTA
TCGTCCTTTAGCCAAGACAAGGCAACAGAATATCAGCATGCAACGGCAAG
AAAACCTTCGTTGGGTGTCAGAGCTCTCTTACGTAGAAGAGAAAGAACAA
TGGCAAGAACAAATCAATCAGCTAAAGAAACAGCTTGATTTTAGTGTCAG
TATTTGTCAGACTTTGATGCAAGACCAGCAGACTCTATCTTGTCTGCTAC
AAACTCTTCTCACGGGTCCTTACAGTGTTATGCCCAGCAATGTTGCATCT
CCTCAAGTACACTTCATAATGCACCAGTTGAACCAGTGCTATACTCAGCT
AACATGGCAACAGAATAATGTTCAGAGGTTGAAACAAATGCTAAATGAAC
TTATGCGCCAGCAAAATCAGCATCCAGAAAAACCTGGAGGCAAGGAAAGA
GGCAGTAGTGCATCGCACCCTCCTTCTCCCAGTTTATTTTGTCCTTTCAG
CTTTCCAACACAGCCTGTAAATCTCTTCAATATACCTGGATTTACTAACT
TTTCATCATTTGCACCAGGTATGAATTTCAGCCCTTTATTTCCTTCTAAT
TTTGGAGATTTTTCTCAGAATATCTCTACACCCAGTGAACAGCAGCAACC
CTTAGCCCAGAATTCTTCAGGAAAAACAGAATATATGGCTTTTCCAAAAC
CTTTTGAAAGCAGTTCCTCTATTGGAGCAGAGAAACCAAGGAATAAAAAA
CTGCCTGAAGAGGAGGTGGAAAGCAGTAGGACACCATGGTTATATGAACA
AGAAGGTGAAGTAGAGAAACCATTTATCAAGACTGGATTTTCAGTGTCTG
TAGAAAAATCTACAAGTAGTAACCGCAAAAATCAATTAGATACAAACGGA
AGAAGACGCCAGTTTGATGAAGAATCACTGGAAAGCTTTAGCAGTATGCC
TGATCCAGTAGATCCAACAACAGTGACTAAAACATTCAAGACAAGAAAAG
CGTCTGCACAGGCCAGCCTGGCATCTAAAGATAAAACTCCCAAGTCAAAA
AGTAAGAAGAGGAATTCTACTCAGCTGAAAAGCAGAGTTAAAAACATCAG
GTATGAAAGTGCCAGTATGTCTAGCACATGTGAACCTTGCAAAAGTAGGA
ACAGACATTCAGCCCAGACTGAAGAGCCTGTTCAAGCAAAAGTATTCAGC
AGAAAGAATCATGAGCAACTGGAAAAAATAATAAAATGTAATAGGTCTAC
AGAAATATCTTCAGAAACTGGGAGTGATTTTTCCATGTTTGAAGCTTTGC
GAGATACTATTTATTCTGAAGTAGCTACATTAATTTCTCAAAATGAATCT
CGTCCACATTTTCTTATTGAACTCTTCCATGAGCTGCAGCTACTAAACAC
AGACTACTTGAGACAGAGGGCTTTATATGCATTGCAGGACATAGTATCCA
GACATATTTCTGAGAGCCATGAAAAAGGAGAAAATGTAAAGTCAGTAAAC
TCTGGTACTTGGATAGCATCAAACTCAGAACTTACTCCTAGTGAGAGCCT
TGCTACTACTGATGATGAAACTTTTGAGAAGAACTTTGAAAGAGAAACCC
ATAAAATAAGTGAGCAAAATGATGCTGATAATGCTAGTGTCCTGTCTGTA
TCATCAAATTTTGAGCCTTTTGCAACAGATGATCTAGGTAACACCGTGAT
TCACTTAGATCAAGCATTAGCCAGAATGAGAGAATATGAGCGTATGAAGA
CTGAGGCTGAAAGTAACTCAAATATGAGATGCACCTGCAGGATTATTGAG
GATGGAGATGGTGCTGGTGCAGGTACTACAGTTAATAATTTAGAAGAAAC
TCCCGTTATTGAAAATCGTAGTTCACAACAACCTGTAAGTGAAGTTTCTA
CCATCCCATGTCCTAGAATTGATACTCAGCAGCTGGACCGGCAAATTAAA
GCAATTATGAAAGAAGTCATTCCTTTTTTGAAGGAGCACATGGATGAAGT
ATGCTCCTCGCAGCTTCTAACTTCAGTAAGGCGCATGGTTTTGACCCTTA
CCCAGCAAAATGATGAGAGCAAAGAGTTTGTAAAGTTCTTTCATAAACAA
CTTGGAAGTATATTACAGGATTCACTGGCAAAATTTGCTGGCAGAAAACT
GAAAGACTGTGGAGAAGATCTTCTTGTAGAGATATCTGAAGTGTTGTTCA
ATGAATTGGCTTTCTTTAAGCTTATGCAAGATTTGGATAATAATAGTATA
ACTGTTAAACAGAGATGCAAAAGGAAAATAGAAGCAACTGGAGTGATACA
ATCTTGTGCCAAAGAGGCTAAAAGGATTCTTGAAGATCATGGCTCACCTG
CTGGAGAGATTGATGATGAAGACAAAGACAAGGATGAAACTGAAACAGTT
AAGCAGACTCAAACATCTGAGGTGTATGATGGTCCCAAAAATGTAAGATC
TGATATTTCTGATCAAGAGGAAGATGAAGAAAGTGAAGGATGTCCAGTGT
CTATTAATTTGTCTAAAGCTGAAACTCAGGCTTTAACTAATTATGGAAGT
GGAGAAGATGAAAATGAGGATGAAGAAATGGAAGAATTTGAAGAAGGCCC
TGTGGATGTCCAGACTTCCCTCCAGGCTAACACTGAAGCTACTGAAGAAA
ATGAACATGATGAACAGGTCCTACAACGTGACTTTAAAAAGACAGCAGAA
AGCAAAAATGTCCCATTGGAACGAGAAGCCACTAGTAAAAATGACCAAAA
TAACTGTCCTGTGAAACCCTGTTACCTCAATATCTTGGAAGATGAGCAAC
CTTTAAATAGTGCTGCCCATAAGGAGTCACCTCCTACTGTTGATTCAACT
CAACAGCCTAACCCTTTGCCGTTACGTTTACCTGAAATGGAACCCTTAGT
GCCTAGAGTCAAAGAAGTTAAATCTGCTCAGGAAACTCCTGAAAGCTCTC
TGGCTGGAAGTCCTGATACTGAATCTCCAGTGTTAGTGAATGACTATGAA
GCAGAATCTGGTAATATAAGTCAAAAGTCTGATGAAGAAGATTTTGTAAA
AGTTGAAGATTTACCACTGAAACTGACAATATATTCAGAGGCAGATCTAA
GAAAGAAAATGGTAGAAGAAGAACAGAAAAACCATTTATCTGGTGAAATA
TGTGAAATGCAGACCGAAGAATTAGCTGGAAATTCTGAGACACTAAAAGA
ACCTGAAACGGTGGGAGCCCAGAGTATATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMATGGG
PFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVES
DKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQR
INFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDA
STSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIV
SRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKF
LKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLAL
QHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDS
QPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEK
KQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNG
ESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELREL
VHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVAST
WNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNVPPSLDCRYN
REGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFE
QKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDT
KQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKL
IDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTS
KSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGL
AETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDG
YLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNN
CPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQL
KKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMH
QLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPP
SPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNI
STPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVES
SRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEE
SLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQ
LKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLE
KIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIEL
FHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASN
SELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFA
TDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAG
TTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIP
FLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDS
LAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKR
KIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEV
YDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDE
EMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLER
EATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPL
RLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQ
KSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEEL
AGNSETLKEPETVGAQSI*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5-FRT/TO-GFP PCM1 ISOFORM2860.87 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU31428
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_006197.3 CDS: ; a1789g (M597V), c2352t (silent) - present in EST and many other published RNA seqs.
• Low Temp: No