cDNA Clone MARK1

• Plasmid: pcDNA5-FRT/TO-HA MARK1 D200A
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA MARK1 D200A
• Genbank: NM_018650.3
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCTGTGGATGGATATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCATTACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAATTTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAACTCAGCTAAATCCTACCAGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCCTAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCGAGTGGGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTAGGCAGATTGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAACCTTCTCCTTGATGGTGATATGAATATTAAAATTGCTGCCTTTGGTTTTAGTAATGAATTTACAGTTGGGAACAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGTATGATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGTCAGTGGCTCCTTGCCTTTCGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATGTCCACAGACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAACAAATAATGAAAGATCGATGGATGAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGATCCGGATTTCAATGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAATGATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAAACCACCTGAATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAACAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAATCAGAAGCAGCGGCGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACAGTGTGGAAAGTGAACAGAAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGGATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGTAACAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGATACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCATATCTTCTGCAGGCTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCACCAGAAGTCCATGTCCACTTCTGGTCATCCTATTAAAGTCACACTGCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAGTGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGGGAGCTCAAGCCGAAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCACCTGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGGTATAATAAGCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAGTGGCAGAACCGACACCTCAAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCTTTGCGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGACATGATGAGAGAAATCCGAAAAGTGTTAGATGCAAATAACTGTGATTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGCTAGACAGGATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTTCGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAATGAGCTTAAGCTGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQ
NIPRCRNSITSATDEQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAV
KIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEY
ASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENL
LLDGDMNIKIAAFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEV
DVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLL
KKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDI
MVTMGFARDEINDALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQ
RSRPSSDLNNSTLQSPAHLKVQRSISANQKQRRFSDHAGPSIPPAVSYTK
RPQANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTI
PSNNVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGS
SVASAVPSARPRHQKSMSTSGHPIKVTLPTIKDGSEAYRPGTTQRVPAAS
PSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGPPASPSHET
GAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERD
KEEGKDSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERF
LLFCVHGDARQDSLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASK
IANELKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31425
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee