cDNA Clone PCTK1

• Plasmid: pBabe.puro-FLAG PCTK1
• Parent Plasmid: pBABE puro FLAG
• Expressed: FLAG PCTK1
• Genbank: NM_006201
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACT
CCGAGGTGGCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGATAG
GCCTGGATGAGAGTGGTGGTGGTGGCGGCAGTGACCCTGGAGAGGCCCCC
ACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAGCTC
TGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGGAGA
GTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGCAGTCTCCAGTGAGA
GTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACATCAA
CAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACCTGG
AGAAGCTGACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGCCGC
CTCCGTCGTGTCAGCCTATCTGAGATTGGCTTTGGGAAACTGGAGACCTA
CATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACAAAG
GCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGATCAGACTG
GAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCTACT
CAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCCACA
CGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTGAAG
CAGTACCTGGATGACTGTGGGAACATCATCAACATGCACAACGTGAAACT
GTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAGAAGG
TGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGGGGA
GAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCCAAC
AAAGACATACTCCAATGAGGTGGTGACACTGTGGTACCGGCCCCCTGACA
TCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGTGGGGTGTG
GGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGGCTC
CACGGTGGAGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCCCAA
CTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGACATAC
AACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCGACT
TGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGGGTC
GAAATCGGATCTCCGCAGAGGATGCCATGAAACATCCATTCTTCCTCAGT
CTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGCACT
AAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGATGC
CTGACTCAGGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAACTC
GAG

Amino Acid Sequence

MDYKDDDDKGSGEFMDRMKKIKRQLSMTLRGGRGIDKTNGAPEQIGLDES
GGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMGSDGESDQAS
ATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTL
NSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKL
TDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSL
TLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVLHRD
LKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGS
TDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETW
PGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRIS
AEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRSSSMPDSGR
PAFRVVDTEF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31411
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Retrovirus vector providing the viral package signal, transcription and processing elements and target gene. For expression in mammalian cells.