cDNA Clone TUG

• Plasmid: pGEX6P-TUG
• Parent Plasmid: pGEX6P1
• Expressed: GST-TUG
• Genbank: NM_024083.2*
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCGGCCCCGGCAGGCGGCGGAGGCTCCGCGGTGTCGGTGCT
GGCCCCGAACGGCCGGCGCCACACGGTGAAGGTGACGCCGAGCACCGTGC
TGCTTCAGGTTCTGGAGGACACGTGCCGGCGGCAGGACTTCAACCCCTGT
GAATATGATCTGAAGTTTCAGAGGAGCGTGCTCGACCTTTCTCTCCAGTG
GAGATTTGCCAACCTGCCCAACAATGCCAAGCTGGAGATGGTGCCCGCTT
CCCGGAGCCGTGAGGGGCCTGAGAACATGGTTCGCATCGCTTTGCAGCTG
GACGATGGCTCGAGGTTGCAGGACTCTTTCTGTTCAGGCCAGACCCTCTG
GGAGCTTCTCAGCCATTTTCCACAGATCAGGGAGTGCCTGCAGCACCCCG
GCGGGGCCACCCCAGTCTGCGTGTACACGAGGGATGAGGTGACGGGTGAA
GCTGCCCTGCGGGGCACGACGCTGCAGTCGCTGGGCCTGACCGGGGGCAG
CGCCACCATCAGGTTTGTCATGAAGTGCTACGACCCCGTGGGCAAGACCC
CAGGAAGCCTGGGCTCGTCAGCGTCGGCTGGCCAGGCAGCCGCCAGCGCT
CCACTTCCCTTGGAATCTGGGGAGCTCAGCCGCGGCGACTTGAGCCGTCC
GGAGGACGCGGACACCTCAGGGCCCTGCTGCGAGCACACTCAGGAGAAGC
AGAGCACAAGGGCACCCGCAGCTGCCCCCTTTGTTCCTTTCTCGGGTGGG
GGACAGAGACAGGGGGGCCCTCCTGGGCCCACGAGGCCTCTGACATCATC
TTCAGCTAAGTTGCCGAAGTCCCTCTCCAGCCCTGGAGGCCCCTCCAAGC
CAAAGAAGTCCAAGTCGGGCCAGGATCCCCAGCAGGAGCAGGAGCAGGAG
CGGGAGCGGGATCCCCAGCAGGAGCAGGAGCGGGAGCGGCCCGTGGACCG
GGAGCCCGTGGACCGGGAGCCGGTGGTGTGCCACCCCGACCTGGAGGAGC
GGCTGCAGGCCTGGCCAGCGGAGCTGCCTGATGAGTTCTTTGAGCTGACG
GTGGACGACGTGAGAAGACGCTTGGCCCAGCTCAAGAGTGAGCGGAAGCG
CCTGGAAGAAGCCCCCTTGGTGACCAAGGCCTTCAGGGAGGCGCAGATAA
AGGAGAAGCTGGAGCGCTACCCAAAGGTGGCTCTGAGGGTCCTGTTCCCC
GACCGCTACGTCCTACAGGGCTTCTTCCGCCCCAGCGAGACAGTGGGGGA
CTTGCGAGACTTCGTGAGGAGCCACCTGGGGAACCCCGAGCTGTCATTTT
ACCTGTTCATCACCCCTCCAAAAACAGTCCTGGACGACCACACGCAGACC
CTCTTTCAGGCGAACCTCTTCCCGGCCGCTCTGGTGCACTTGGGAGCCGA
GGAGCCGGCAGGTGTCTACCTGGAGCCTGGCCTGCTGGAGCATGCCATCT
CCCCATCTGCGGCCGACGTGCTGGTGGCCAGGTACATGTCCAGGGCCGCC
GGGTCCCCTTCCCCATTGCCAGCCCCTGACCCTGCACCTAAGTCTGAGCC
AGCTGCTGAGGAGGGGGCGCTGGTCCCCCCTGAGCCCATCCCAGGGACGG
CCCAGCCCGTGAAGAGGAGCCTGGGCAAGGTGCCCAAGTGGCTGAAGCTG
CCGGCCAGCAAGAGGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAPAGGGGSAVSVLAPNG
RRHTVKVTPSTVLLQVLEDTCRRQDFNPCEYDLKFQRSVLDLSLQWRFAN
LPNNAKLEMVPASRSREGPENMVRIALQLDDGSRLQDSFCSGQTLWELLS
HFPQIRECLQHPGGATPVCVYTRDEVTGEAALRGTTLQSLGLTGGSATIR
FVMKCYDPVGKTPGSLGSSASAGQAAASAPLPLESGELSRGDLSRPEDAD
TSGPCCEHTQEKQSTRAPAAAPFVPFSGGGQRQGGPPGPTRPLTSSSAKL
PKSLSSPGGPSKPKKSKSGQDPQQEQEQERERDPQQEQERERPVDREPVD
REPVVCHPDLEERLQAWPAELPDEFFELTVDDVRRRLAQLKSERKRLEEA
PLVTKAFREAQIKEKLERYPKVALRVLFPDRYVLQGFFRPSETVGDLRDF
VRSHLGNPELSFYLFITPPKTVLDDHTQTLFQANLFPAALVHLGAEEPAG
VYLEPGLLEHAISPSAADVLVARYMSRAAGSPSPLPAPDPAPKSEPAAEE
GALVPPEPIPGTAQPVKRSLGKVPKWLKLPASKR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31377
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_024083.2 CDS: t755a (L252Q) = misc_difference (see BC018722); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 87018 Daltons