cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pcDNA5-FRT/TO-GFP AS160(B) long
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-AS160(B)
• Genbank: NM_014832.2*
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCC
CCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAA
GCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGACCAC
AGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCG
CAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGG
TGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGC
GCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGC
GGTATTCATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACA
ACAGCCACGACCTCACCTACTTTGCCTACCTGATCAAGGCGCAGCCCGAC
GACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACCCCAG
CCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCA
TGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAAC
TCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAA
GAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGC
ACGAACAGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGTCCGGACCCA
GGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGA
CTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTACCTG
CCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAG
CGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCG
GTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGA
AATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCC
TCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGA
GATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATT
TTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTT
GGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTG
TTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGA
CTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGTGCCAAGACG
CAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTG
TGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGA
GGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGA
GTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGAT
TTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACA
TCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCCAGAAAATGCA
ACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAG
ATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAA
TGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTT
GCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCC
AGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCG
ACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCA
AGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCG
TTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGCAATCTTTCGT
CAGTTCGACGCATGTACAAGGAGAGTAATTCTTCCTCCAGTCTTCCAAGT
CTTCACACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTCCTGAA
AAGCTTTTACCAGAATTCAGGTAGACTGTCCCCACAGTATGAAAATGAAA
TCAGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAG
TCCCTAAGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCG
GCAGCGCATTTTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAG
CAATGCAACAGCAAGATGGATTGGACAGGAACGAGCTGCTGCCACTGTCC
CCCCTCTCTCCAACCATGGAGGAGGAACCGCTGGTTATATTCCTGTCTGG
GGAGGATGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGA
GGAGCTTGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATG
GAAAAAGAAAACCAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAG
AAAAGTTAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCT
TAATAACTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGT
GATATGGAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCG
ACGAGGAGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACA
GATTGCCTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTG
AGGCAGCTCACTGCTCAGCAGCATGCGATTCTTGTGGATTTAGGAAGGAC
GTTTCCTACTCACCCTTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGT
CACTGTTTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGA
TACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAG
TGAAGAGCAAGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCT
TCCGCAAGCAGTACAGACCTGACATGATGTCGCTGCAGATTCAAATGTAC
CAGCTGTCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCT
TGAAGAAAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCA
CATTGTTTGCCTCTCAGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGAT
ATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCACTCAGCCT
ACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATA
TTGTTGAGTTTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATG
GAAAAAATTATTACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCA
TGCCTATGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTT
CATATTCCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAAT
AGCCAACTGAAAAGACAAAACATGGACCTCCTAGAAAAATTACAGGTAGC
TCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCTTTTGACGA
GAGAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATG
GCTTATCAAAAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGC
TCTAGCCAATTGTGACCTGTTGCTGAGAGACCTAAACTGCAACCCTAACA
ACAAAGCCAAGATAGGAAATAAGCCATAAggatcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMEPPSC
IQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLP
WLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTS
PSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMAC
HVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLY
CGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLE
VVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGF
DEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRT
MLFQVGRFEINLISPDTKSVVLEKNFKDISPCSQGIKHVDHFGFICRESP
EPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEAC
PMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVS
DQEENELVILHLRQLCEAKQKTHVHIGEGPATISNSTIPENATSSGRFKL
DILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSP
GDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNL
QDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAP
SFTAPSFLKSFYQNSGRLSPQYENEISDGEGRKRTSSTCSNESLSVGGTS
VTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEE
EPLVIFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLE
ASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTL
LKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLRQLTAQQH
AILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFV
AGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHD
YHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTE
VIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVF
EMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNM
DLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQ
LRKLLPADALANCDLLLRDLNCNPNNKAKIGNKP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31363
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in cloned AS160 (B) (long form) compared with NM_014832.2: ; S387P, S537A, K957R, V819I, V1275A, delta R733-S740. These differences are present in all AS160(B) (long form) DU clones.