cDNA Clone ZNRF2

• Plasmid: pcDNA5-FRT/TO-GFP ZNRF2 C199A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP ZNRF2 C199A
• Genbank: NM_147128.3 GI:40316919
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGGCGCCAAACAGAGCGGCCCGGCCGCCGCTAACGGCCGCAC
GCGCGCGTACTCGGGCTCGGATCTACCTTCCAGTAGCAGCGGAGGCGCCA
ATGGGACCGCGGGCGGCGGCGGGGGCGCTCGGGCCGCCGCCGCGGGGAGG
TTCCCGGCTCAGGTGCCCAGCGCGCACCAGCCCAGCGCCTCCGGCGGCGC
CGCGGCGGCCGCGGCGGCCCCGGCAGCCCCGGCGGCCCCGCGCAGCCGCT
CCCTCGGCGGGGCCGTGGGGAGCGTGGCGTCGGGGGCCCGCGCGGCGCAG
TCCCCCTTCAGCATCCCGAACAGCAGCAGCGGCCCGTACGGCTCGCAGGA
CTCGGTGCACAGCAGCCCTGAGGACGGCGGCGGCGGCCGGGACCGGCCGG
TGGGCGGGAGCCCCGGCGGGCCGCGCCTGGTGATCGGCTCCTTACCAGCT
CACCTCTCGCCGCACATGTTTGGAGGATTTAAGTGCCCTGTATGCTCAAA
ATTTGTATCCTCAGATGAAATGGATTTGCATCTTGTAATGTGTTTAACAA
AGCCACGAATAACCTATAATGAGGATGTACTGAGTAAAGATGCTGGGGAA
GCTGCAATATGCCTTGAAGAATTGCAGCAGGGAGATACTATAGCACGACT
GCCTTGTCTATGCATATATCATAAAGGCTGCATAGATGAATGGTTTGAAG
TAAATAGATCTTGCCCTGAGCACCCTTCAGATTAAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMGAKQS
GPAAANGRTRAYSGSDLPSSSSGGANGTAGGGGGARAAAAGRFPAQVPSA
HQPSASGGAAAAAAAPAAPAAPRSRSLGGAVGSVASGARAAQSPFSIPNS
SSGPYGSQDSVHSSPEDGGGGRDRPVGGSPGGPRLVIGSLPAHLSPHMFG
GFKCPVCSKFVSSDEMDLHLVMCLTKPRITYNEDVLSKDAGEAAICLEEL
QQGDTIARLPCLCIYHKGCIDEWFEVNRSCPEHPSD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31355
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All ZNRF2 plasmids MUST be grown at 30 degrees C or less to prevent recombination.