cDNA Clone Atxn1

• Plasmid: pcDNA5-FRT/TO-HA Atxn1 S775A
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA Atxn1 S775A
• Genbank: NM_001128164.1 GI:189491747
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAA
GAAGCGCGAGATCCCCGCCACCAGCCGGTCCTCCGAGGAGAAGGCCCCTA
CCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCCGGGC
AACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGAC
CTCGGTGGAGCTTGGTTTACAACAGGGAATAGGTTTACACAAAGCATTGT
CCACAGGGCTGGACTACTCCCCGCCCAGCGCTCCCAGGTCTGTCCCCGTG
GCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGT
GTCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGT
CCTCCCAATACAGTGGAACCTATGCCAGCTTCATCCCATCACAGCTGATC
CCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAGGGGC
CACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGG
CCAACATGGGCAGTCTGAGCCAGACGCCGGGACACAAGGCTGAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCAGCATCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCTCCGG
GGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTC
CACATTTCCAGTTCTCCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGC
CATCCCCGTCCACCTCCACCCCCACCAGACGATGATCCCACACACGCTCA
CCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGC
CACTTTGTCCCTCGGGAGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCA
GCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGAGATGGAGAAGAGCC
GGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGC
GGCAAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCC
GAGCCCCTCAGACTACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTG
TGATGGTCCTGCCCAACAGCAACACGCCCGCAGCTGACCTGGAGGTGCAA
CAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGTGG
CCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCACCCCACA
CGGTCATTCAGACCACACACAGTGCTTCAGAGCCACTCCCGGTGGGACTG
CCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCT
GAGCGGCCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACC
TGGTGATCCCCGGCACACAGCCCCTGCTCATCCCGGTCGGCAGCACTGAC
ATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTCACGTCATCCCCCCAGTT
TGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAGAGCGAGA
ACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTG
CAGGCCCAGATCCACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGC
GGCTCCCCCTACGCTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGT
TGGCCAACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTC
ATCCAGAGTGCAGAGATAAGCAACGACCTGAAGATCGACTCCAGCACCGT
AGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATACAGTTCG
CCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTAT
CCTTTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAAC
CAGCCAGCTCTTTGATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCT
GCATCTCGCTTACCCTCAAGAACCTGAAGAACGGCTCTGTTAAAAAGGGC
CAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCCGACGG
CCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGAATCAACC
AGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAG
AAAATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAA
GCCCGCGGCAACGAGGAAGAGGAGGTGGGCGGCGCCAGAGAGCCGCAAAC
TGGAGAAGTCAGAAGACGAACCACCTTTGACTCTTCCTAAGCCTTCTCTA
ATTCCTCAGGAGGTTAAGATTTGCATTGAAGGCCGGTCTAATGTAGGCAA
GTAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDN
HRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDY
SPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSG
TYASFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSL
SQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQQQQQQQHLSRAPGLITPG
SPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHTLTLGPPS
QVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAP
SSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPN
SNTPAADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTT
HSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGT
QPLLIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEA
LVTQAAYPAMVQAQIHLPVVQSVASPAAAPPTLPPYFMKGSIIQLANGEL
KKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHR
AQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTL
KNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQM
LSENGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWAAPESRKLEKSED
EPPLTLPKPSLIPQEVKICIEGRSNVGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31161
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee