cDNA Clone UFC1

• Plasmid: pGEX6P-2-UFC1
• Parent Plasmid: pGEX6P2
• Expressed: GST-UFC1
• Genbank: NM_016406.3 GI:156415999*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGATGAAGCCACGCGACGTGTTGTGTCTGAGATCCCGGT
GCTGAAGACTAACGCCGGACCCCGAGATCGTGAGTTGTGGGTGCAGCGAC
TGAAGGAGGAATATCAGTCCCTTATCCGGTATGTGGAGAACAACAAGAAT
GCTGACAACGATTGGTTCCGACTGGAGTCCAACAAGGAAGGAACTCGGTG
GTTTGGAAAATGCTGGTATATCCATGACCTCCTGAAATATGAGTTTGACA
TCGAGTTTGACATTCCTATCACATGTCCTACTACTGCCCCAGAAATTGCA
GTTCCTGAGCTGGATGGAAAGACAGCAAAGATGTACAGGGGTGGCAAAAT
ATGCCTGACGGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCCAAAT
TTGGACTAGCTCATCTCATGGCTCTGGGGCTGGGTCCATGGCTGGCAGTG
GAAATCCCTGATCTGATTCAGAAGGGCGTCATCCAACACAAAGAGAAATG
CAACCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLKTN
AGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKC
WYIHDLLKYEFDIEFDIPITCPTTAPEIAVPELDGKTAKMYRGGKICLTD
HFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31145
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_016406.3 CDS: a269g (Y90C) (matches BC005187.1 GI:13528770); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 46221 Daltons