cDNA Clone TH

• Plasmid: pcDNA5-FRT/TO-GFP TH isoform a S19A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP TH isoform a S19A
• Genbank: NM_199292.2 GI:88900500*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCCACCCCCGACGCCACCACGCCACAGGCCAAGGGCTTCCG
CAGGGCCGTGGCTGAGCTGGACGCCAAGCAGGCAGAGGCCATCATGGTAA
GAGGGCAGGGCGCCCCGGGGCCCAGCCTCACAGGCTCTCCGTGGCCTGGA
ACTGCAGCCCCAGCTGCATCCTACACCCCCACCCCAAGGTCCCCGCGGTT
CATTGGGCGCAGGCAGAGCCTCATCGAGGACGCCCGCAAGGAGCGGGAGG
CGGCGGTGGCAGCAGCGGCCGCTGCAGTCCCCTCGGAGCCCGGGGACCCC
CTGGAGGCTGTGGCCTTTGAGGAGAAGGAGGGGAAGGCCATGCTAAACCT
GCTCTTCTCCCCGAGGGCCACCAAGCCCTCGGCGCTGTCCCGAGCTGTGA
AGGTGTTTGAGACGTTTGAAGCCAAAATCCACCATCTAGAGACCCGGCCC
GCCCAGAGGCCGCGAGCTGGGGGCCCCCACCTGGAGTACTTCGTGCGCCT
CGAGGTGCGCCGAGGGGACCTGGCCGCCCTGCTCAGTGGTGTGCGCCAGG
TGTCAGAGGACGTGCGCAGCCCCGCGGGGCCCAAGGTCCCCTGGTTCCCA
AGAAAAGTGTCAGAGCTGGACAAGTGTCATCACCTGGTCACCAAGTTCGA
CCCTGACCTGGACTTGGACCACCCGGGCTTCTCGGACCAGGTGTACCGCC
AGCGCAGGAAGCTGATTGCTGAGATCGCCTTCCAGTACAGGCACGGCGAC
CCGATTCCCCGTGTGGAGTACACCGCCGAGGAGATTGCCACCTGGAAGGA
GGTCTACACCACGCTGAAGGGCCTCTACGCCACGCACGCCTGCGGGGAGC
ACCTGGAGGCCTTTGCTTTGCTGGAGCGCTTCAGCGGCTACCGGGAAGAC
AATATCCCCCAGCTGGAGGACGTCTCCCGCTTCCTGAAGGAGCGCACGGG
CTTCCAGCTGCGGCCTGTGGCCGGCCTGCTGTCCGCCCGGGACTTCCTGG
CCAGCCTGGCCTTCCGCGTGTTCCAGTGCACCCAGTATATCCGCCACGCG
TCCTCGCCCATGCACTCCCCTGAGCCGGACTGCTGCCACGAGCTGCTGGG
GCACGTGCCCATGCTGGCCGACCGCACCTTCGCGCAGTTCTCGCAGGACA
TTGGCCTGGCGTCCCTGGGGGCCTCGGATGAGGAAATTGAGAAGCTGTCC
ACGCTGTACTGGTTCACGGTGGAGTTCGGGCTGTGTAAGCAGAACGGGGA
GGTGAAGGCCTATGGTGCCGGGCTGCTGTCCTCCTACGGGGAGCTCCTGC
ACTGCCTGTCTGAGGAGCCTGAGATTCGGGCCTTCGACCCTGAGGCTGCG
GCCGTGCAGCCCTACCAAGACCAGACGTACCAGTCAGTCTACTTCGTGTC
TGAGAGCTTCAGTGACGCCAAGGACAAGCTCAGGAGCTATGCCTCACGCA
TCCAGCGCCCCTTCTCCGTGAAGTTCGACCCGTACACGCTGGCCATCGAC
GTGCTGGACAGCCCCCAGGCCGTGCGGCGCTCCCTGGAGGGTGTCCAGGA
TGAGCTGGACACCCTTGCCCATGCGCTGAGTGCCATTGGCTAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPTPDA
TTPQAKGFRRAVAELDAKQAEAIMVRGQGAPGPSLTGSPWPGTAAPAASY
TPTPRSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEE
KEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGG
PHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDK
CHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYT
AEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDV
SRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPE
PDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVE
FGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQ
TYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAV
RRSLEGVQDELDTLAHALSAIG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31135
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_199292.2 CDS:; g334a (V112M) - present in other published sequences.