cDNA Clone ITCH

• Plasmid: pMal6P-1 ITCH
• Parent Plasmid: pMAL6P
• Expressed: MBP-ITCH
• Genbank: NM_031483
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACTGTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTTACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGATGTTTTGTTGGGAACTGCTGCATTA
GATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG
ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACC
AATGGTGAAACTACATGTTCAGAAAGTGCTTCTCAGAATGATGATGGCTC
CAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTG
AAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCA
TCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACG
ACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCAT
CTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA
AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCC
TCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTC
AAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGA
GTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGA
ACCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTT
ATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG
GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA
AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATT
TATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCA
CCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAA
CCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAAT
TAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGAT
GGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTATATAGA
TCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG
TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAA
CTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTT
TGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAA
GACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGT
GTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAAT
GTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAA
ACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT
GGCAGATTCATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGG
TTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCA
AGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGGGTT
AAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGA
CAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTG
GCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTA
GCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT
TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATG
CAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAAT
GACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACCAGCAAACA
AATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAA
TGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA
TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAA
AGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCC
TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTG
TTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGLEVLFQGPLGSMSDSGSQLGSMGSLTMKSQLQITVISAKLKE
NKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHF
RVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTET
IGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSD
DPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNG
SPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNP
VTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMG
RIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGN
QDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQ
GQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQI
AYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQD
LRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCL
QINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPV
GLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKP
NGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQY
FDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNE
KRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCF
NRLDLPPYKSYEQLKEKLLFAIEETEGFGQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31128
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee