cDNA Clone Capicua (CIC) (Drosophila)

• Plasmid: pcDNA5-FRT/TO-GFP Drosophila Capicua S467A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Genbank: Closest match: NM_080253.2 GI:24648371*
• Tag: GFP
• Species: Drosophila Melanogaster

Sequence of Insert

GGATCCATGAATGCGTTCCAGGACTTTGAGCTGGGGGCCAAACTATATCT
GCAGTGCTTATTATCGCTGAGCAGCTCCCGATCTGCCACGCCATCCTACA
CCTCACCTGTTAACCACGCTGGAGCTTCTCCTCTGAATGCCATTGCCCAT
TCGCCGGTGAATGTGTCCGCCACTCATAGGCAGAACTTTTTCACACCCAT
TGCCAACCAGTCGCAGCAGCAACAACAGCAACAGCAGCAACAGCCTGTGG
CGGTACCGCTCGACTCCAAGTGGAAAACAACACCCAGTCCGGTGCTCTAC
AATGCCAACAACAACAGCAGCAATAACAACACCAGCAGCAACAACAACAA
CAACAACAGTAACTGGGAGGTGGGCAGCAATAGCAATACTCATGTTGCAG
CTACGGCAGCAGCAACATCAACAGTTGGCGCTCAACCACTGCCGCCGCAA
ACGACACCCGTTTCGCTGGTGATGCACGCCCCACCGCCGCAGCAGCACCC
ACTGCAGCAGCAGCACCACCATCACCAACCGCCTCCACCGCCACCTGCTA
GTTTGCCAGCGCCATCGGCACCACCCACCAGCGGCAGCAGCAGCAGCCAC
AACAGCGTGGGCCATGCCACCAGCGTTATACGCATTTCGAGCAGCCAACA
GCAACATCAGCAGCAGCAACAGCACCAACAGCACCAACAGCAGGCACATC
CACATGTGGTGGTGAGCGGCGGACAGACTTTTCATCCCGTAATCGTGGAC
GCTACTCAGCTATCGGTGCCGCTGCCACCCACAACGGTTTCATTTCATCA
GCCCAACACGCCCACCTCAACAGCGGCATCAGTGGCTTCAATGAGCCAGG
ACAAGATGCTGGCAAAAAACGGCTACAATGCGCCCTGGTTCAAGCTGTTG
CCGCATATGACGCCAATGAGCAAGGCGTCGCCAGCTCCGGTCACTCCGAC
CCTAACCACATCGGCCAGCAGTTACAACGTGGTGATGATGCAACAGCAGC
AGCAGCATCAGCAGCTTCAACAACAGCAGCAACTGCAGCAGCAGCAACAG
TCGCCACCCCAGATGCCGCTGAACCACAACAACAATCATCTGATTGTGTC
CGCCCCACTAAGCTCCCCGGGCAAGCCACTCAACTGCTCAATGAACGATG
CCAAGGTGGCGGCGGCAGCAGCAGCAGCCGCAGTTGCCAACCAGAGGCAA
AAGCAGCAGCAGGAGGAGCCGGACGATCAGCTGGACGATGATGTGTTTGA
GACCACAACGCCCGGTATCAGCGCCAACAGCAAAAAACAAACTGCGGCAA
TGCGGCTGCCCACCCACAACAGTAACATACGAAAGCTGGAGGAATGCCAT
GATGATGGAGCTGCAGGTGCACCAGCCACTTCGGCTGCCAAGCGGAGGAG
TCAAGCGTTGAGCGCCCTGCAGCAACAGCAGCAGCAGCAACAGCAGGCCG
GTGCGGCAGGAACAGCAGCTGGGCAGCCGGCGAACAAGAAAATCCGAAGA
CCCATGAACGCTTTTATGATCTTCTCAAAGAAACATCGGAAAATGGTGCA
CAAGAAGCATCCGAATCAGGACAACCGTACGGTCAGCAAGATTCTGGGCG
AGTGGTGGTACGCCCTGAAGCCAGAGCAAAAGGCTCAGTACCACGAGCTG
GCCAGCTCTGTTAAGGATGCACACTTCAAGCTGCATCCGGAGTGGAAGTG
GTGCTCCAAAGATCGCCGCAAGTCATCCACCTCGACAGCAACGCCTGGTG
GAAAGGCGAGCGGAGCGGCCGGAACAGGTGACGCTAAGCAGCGCCTGGTA
TCGGTGGATGGCTCCGATTCCTTGGAGCACGATATGTGCCCCTCAACGCC
AGGAGGCAGCGGCAGCTGCGGTGGCCAGGGCATATCATCCGATTTGCAGG
GCGATATTATACCGCTGACGATTGACAACTACAATAGCACCTGTGATGAG
GCGCCAACTACGATCTCGATGAAGGGCAATGGCAACGGAAAGCTGATGAA
GAACGAGCTGCCATCGGACGAGGACGAACAGATGCTGGTGGTGGAGGAGG
AGCAGCAACAGCAGACCGTAAAGAAGATTGACCTGCACTGCCGCGAGCGG
GTGAATGATTCGGACATGGACGACACACCCTTTGACTACCGCAAGCAGCA
GCCAGAGGCAAATCAGCGTTCGGCAGAGGAGCACAGTACATCCGGTGCGA
ATGGACAGGCCATAAACGCACCACCGCTCAGCGGAGGAGAGCGCGAGATC
ACACTCAAACCGAAGGCTATCAAAGCACATCCGGTGCTGGAGAGCAACAT
GCTGCCATATACCCAGATGTCCATCTACACGCAGTACACTAGTCCCAAGA
ACCCAATCGGTGTAACACCATTCCAACCCACAGGCGGCGCCTTCAAATCG
ATGCCCATCAGCCCCAAGGGCAGCGGTGGCAAGCCGGAGGACGCTGGCTC
CCTGCAGGCACACATCAAGCAGGAGGACATCAAACAGGAACCGCCATCGC
CATACAAGCTGAACAATGGTTCGGGCTCCGCCTCCGGAGGGGGCGTGGTC
AGTGCTCCGCCGCCGAATAGTGGTAGCGTTGGTGCCATATTCAACTTCAA
TGTGCCAACGGCGACGGCTTTGAGTCAGAAGCAGTTTCACTACCCGATGC
ATCATCCTCATCGCAGTCCCACAGATCTTAGAGCTGCCCACCAGGCGTGT
GTGCCATCGTCGCCAGCGGGCATGGGACTGGGGCATGCGGCCAACATTGC
CACGCCTCCAGCATCGGCGCCGGCCCAGATCATGGGAGGCGGTCCAGCCA
GTCAGAAGATGTTCTTCGCCATGACTCATCCGTATACGTTGCTGCAGCGC
TCCCACCAACCGGGCACCCCCAGCCTAGAGCACTTGCAGCTGGACGCTTT
TGCGCCAGGAGGTTACACCTTGAGGAACCACAATGGCCTGTCTTCCCTGC
CACCGCCCGTGAGTGCGCAACCCACGATGCTGCTGCACGGATACCCGCCC
AGCCATGGTGTCGAACCACCGGCTAGATCGCCATCGTACAAATCAATGCC
ATCTACTCCAAAGTCGGCCACATATCTCATGAGTGCGCCACCGGAGCGAG
GCATGGATGGAGGAATGAGTGGATGTGCATCAGCAGCGGCAAGCGGAGGC
GATGAAAGCGACATAGATGCGGATGGCCAGCAGTTTATTTTGGCACCCAC
GCCTGCGCAGTTGGGTCGAGCTCCACTGCAGAGGAGGAAGAATCTATCTC
AAAGCAAGTCAGAGAGCAATGTGTCCTTTGGCGCTAATCTGGGGGCCAGC
AATGGACAGCACATTAGTCGCAAGCTGCACTCACCCACAATGATGGAATC
CTCATCGCCCATCATAGGCCACGTGAACAGCAGCAACCTCAGCTCGGCCC
TGCCAACGCCCACGTCCTCGACGACGACACCAAACAGTGATGAGCAACTG
CCACTGACGCCGACAACCAGCAGCAGCAACAGTAACTTAAATCAGCAGCA
GCCCAAGTCACCGATGAAAGGTGCTCCAGGATCAACGGCAGCGGCCCTAA
AAAAGAAGAACGATGAGATGAATAACAGTGTGCTCAAGCAGGTAGATTTC
GAAAAGAAATACAAGGCCCTGCCGCAGTTCCAGCCTGAGGATTGCCAGTC
GCCCAGTGCCATTGCTGTGCCCTCATCGCCACGCGTTTACGGCACGAATT
ACCGCAAGAAGAACACGGCTCCACCGCCAGTTCAGAAGCTAATGTGCGAG
GACGACTCCATTGACGAACCAGCATCGGCACCGCCTACGACCACACAGCG
ATTCTTTGGTCCGGACTTTAACAATGAGCTAAAGGACGAACGCCTTGCAG
AACTGGAGAGCTCTGACCAAACGGGCCGCTCACCCAGGACGCCAAAGACA
CCGCTACAAAGCGCTCGGTCCGATGCCAGTGAGAAAGGACACCGCAAGGT
CCTGGAGACGCGTCGCAGCCTGGTCTTGCAGTTGTTTGCCGAGCACGGTA
ACTTCCCCACTGCCCAGGCCACTATGGCCTTTCAGTCTAAGCACAGCGAT
GTCTTTCCGCGCAAGCAAGATCTGCAGCTGAAGATACGAGAGGTGCGCCA
GAAGCTGCTGGGCCAAGCATCTTGCACTCCACACTCGGCGGGCCCCAACA
CGCCGTCCGACTCCAACTCGTCGTCGACCACATTGAGTGCCAGTAGCACC
AGCTTGAATATGCAGACCACCAGTGCGGCAGATGTTTTTCAATATTACTG
AGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMNAFQD
FELGAKLYLQCLLSLSSSRSATPSYTSPVNHAGASPLNAIAHSPVNVSAT
HRQNFFTPIANQSQQQQQQQQQQPVAVPLDSKWKTTPSPVLYNANNNSSN
NNTSSNNNNNNSNWEVGSNSNTHVAATAAATSTVGAQPLPPQTTPVSLVM
HAPPPQQHPLQQQHHHHQPPPPPPASLPAPSAPPTSGSSSSHNSVGHATS
VIRISSSQQQHQQQQQHQQHQQQAHPHVVVSGGQTFHPVIVDATQLSVPL
PPTTVSFHQPNTPTSTAASVASMSQDKMLAKNGYNAPWFKLLPHMTPMSK
ASPAPVTPTLTTSASSYNVVMMQQQQQHQQLQQQQQLQQQQQSPPQMPLN
HNNNHLIVSAPLSSPGKPLNCSMNDAKVAAAAAAAAVANQRQKQQQEEPD
DQLDDDVFETTTPGISANSKKQTAAMRLPTHNSNIRKLEECHDDGAAGAP
ATSAAKRRSQALSALQQQQQQQQQAGAAGTAAGQPANKKIRRPMNAFMIF
SKKHRKMVHKKHPNQDNRTVSKILGEWWYALKPEQKAQYHELASSVKDAH
FKLHPEWKWCSKDRRKSSTSTATPGGKASGAAGTGDAKQRLVSVDGSDSL
EHDMCPSTPGGSGSCGGQGISSDLQGDIIPLTIDNYNSTCDEAPTTISMK
GNGNGKLMKNELPSDEDEQMLVVEEEQQQQTVKKIDLHCRERVNDSDMDD
TPFDYRKQQPEANQRSAEEHSTSGANGQAINAPPLSGGEREITLKPKAIK
AHPVLESNMLPYTQMSIYTQYTSPKNPIGVTPFQPTGGAFKSMPISPKGS
GGKPEDAGSLQAHIKQEDIKQEPPSPYKLNNGSGSASGGGVVSAPPPNSG
SVGAIFNFNVPTATALSQKQFHYPMHHPHRSPTDLRAAHQACVPSSPAGM
GLGHAANIATPPASAPAQIMGGGPASQKMFFAMTHPYTLLQRSHQPGTPS
LEHLQLDAFAPGGYTLRNHNGLSSLPPPVSAQPTMLLHGYPPSHGVEPPA
RSPSYKSMPSTPKSATYLMSAPPERGMDGGMSGCASAAASGGDESDIDAD
GQQFILAPTPAQLGRAPLQRRKNLSQSKSESNVSFGANLGASNGQHISRK
LHSPTMMESSSPIIGHVNSSNLSSALPTPTSSTTTPNSDEQLPLTPTTSS
SNSNLNQQQPKSPMKGAPGSTAAALKKKNDEMNNSVLKQVDFEKKYKALP
QFQPEDCQSPSAIAVPSSPRVYGTNYRKKNTAPPPVQKLMCEDDSIDEPA
SAPPTTTQRFFGPDFNNELKDERLAELESSDQTGRSPRTPKTPLQSARSD
ASEKGHRKVLETRRSLVLQLFAEHGNFPTAQATMAFQSKHSDVFPRKQDL
QLKIREVRQKLLGQASCTPHSAGPNTPSDSNSSSTTLSASSTSLNMQTTS
AADVFQYY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *5 differences at the amino acid level:; Two point mutations: S109N and Q161H; Three insertions: 'QQQ', 'HQQ' and 'DERLA'; ; All of the above except the DERLA insertion are present in other published Drosophila CIC sequences. The DERLA insertion was present in several clones that were sequenced from the cloning of RT-PCR products using first strand cDNA made from both oligo-dT and also a sequence-specific primer.