cDNA Clone PIN1

• Plasmid: pGEX6P-1-PIN1
• Parent Plasmid: pGEX6P2
• Expressed: GST-PIN1
• Genbank: NM_006221.2 GI:56676308
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGGGAGAAGCGCAT
GAGCCGCAGCTCAGGCCGAGTGTACTACTTCAACCACATCACTAACGCCA
GCCAGTGGGAGCGGCCCAGCGGCAACAGCAGCAGTGGTGGCAAAAACGGG
CAGGGGGAGCCTGCCAGGGTCCGCTGCTCGCACCTGCTGGTGAAGCACAG
CCAGTCACGGCGGCCCTCGTCCTGGCGGCAGGAGAAGATCACCCGGACCA
AGGAGGAGGCCCTGGAGCTGATCAACGGCTACATCCAGAAGATCAAGTCG
GGAGAGGAGGACTTTGAGTCTCTGGCCTCACAGTTCAGCGACTGCAGCTC
AGCCAAGGCCAGGGGAGACCTGGGTGCCTTCAGCAGAGGTCAGATGCAGA
AGCCATTTGAAGACGCCTCGTTTGCGCTGCGGACGGGGGAGATGAGCGGG
CCCGTGTTCACGGATTCCGGCATCCACATCATCCTCCGCACTGAGTGAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEEKLPPGWEKRMSRSS
GRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRR
PSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKAR
GDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31006
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 45066 Daltons