cDNA Clone Atxn1

• Plasmid: pcDNA5-FRT/TO-GFP Atxn1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP Atxn1
• Genbank: NM_001128164.1 GI:189491747
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAA
GAAGCGCGAGATCCCCGCCACCAGCCGGTCCTCCGAGGAGAAGGCCCCTA
CCCTGCCCAGCGACAACCACCGGGTGGAGGGCACAGCATGGCTCCCGGGC
AACCCTGGTGGCCGGGGCCACGGGGGCGGGAGGCATGGGCCGGCAGGGAC
CTCGGTGGAGCTTGGTTTACAACAGGGAATAGGTTTACACAAAGCATTGT
CCACAGGGCTGGACTACTCCCCGCCCAGCGCTCCCAGGTCTGTCCCCGTG
GCCACCACGCTGCCTGCCGCGTACGCCACCCCGCAGCCAGGGACCCCGGT
GTCCCCCGTGCAGTACGCTCACCTGCCGCACACCTTCCAGTTCATTGGGT
CCTCCCAATACAGTGGAACCTATGCCAGCTTCATCCCATCACAGCTGATC
CCCCCAACCGCCAACCCCGTCACCAGTGCAGTGGCCTCGGCCGCAGGGGC
CACCACTCCATCCCAGCGCTCCCAGCTGGAGGCCTATTCCACTCTGCTGG
CCAACATGGGCAGTCTGAGCCAGACGCCGGGACACAAGGCTGAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCAGCATCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACCTCAGCAGGGCTCCGG
GGCTCATCACCCCGGGGTCCCCCCCACCAGCCCAGCAGAACCAGTACGTC
CACATTTCCAGTTCTCCGCAGAACACCGGCCGCACCGCCTCTCCTCCGGC
CATCCCCGTCCACCTCCACCCCCACCAGACGATGATCCCACACACGCTCA
CCCTGGGGCCCCCCTCCCAGGTCGTCATGCAATACGCCGACTCCGGCAGC
CACTTTGTCCCTCGGGAGGCCACCAAGAAAGCTGAGAGCAGCCGGCTGCA
GCAGGCCATCCAGGCCAAGGAGGTCCTGAACGGTGAGATGGAGAAGAGCC
GGCGGTACGGGGCCCCGTCCTCAGCCGACCTGGGCCTGGGCAAGGCAGGC
GGCAAGTCGGTTCCTCACCCGTACGAGTCCAGGCACGTGGTGGTCCACCC
GAGCCCCTCAGACTACAGCAGTCGTGATCCTTCGGGGGTCCGGGCCTCTG
TGATGGTCCTGCCCAACAGCAACACGCCCGCAGCTGACCTGGAGGTGCAA
CAGGCCACTCATCGTGAAGCCTCCCCTTCTACCCTCAACGACAAAAGTGG
CCTGCATTTAGGGAAGCCTGGCCACCGGTCCTACGCGCTCTCACCCCACA
CGGTCATTCAGACCACACACAGTGCTTCAGAGCCACTCCCGGTGGGACTG
CCAGCCACGGCCTTCTACGCAGGGACTCAACCCCCTGTCATCGGCTACCT
GAGCGGCCAGCAGCAAGCAATCACCTACGCCGGCAGCCTGCCCCAGCACC
TGGTGATCCCCGGCACACAGCCCCTGCTCATCCCGGTCGGCAGCACTGAC
ATGGAAGCGTCGGGGGCAGCCCCGGCCATAGTCACGTCATCCCCCCAGTT
TGCTGCAGTGCCTCACACGTTCGTCACCACCGCCCTTCCCAAGAGCGAGA
ACTTCAACCCTGAGGCCCTGGTCACCCAGGCCGCCTACCCAGCCATGGTG
CAGGCCCAGATCCACCTGCCTGTGGTGCAGTCCGTGGCCTCCCCGGCGGC
GGCTCCCCCTACGCTGCCTCCCTACTTCATGAAAGGCTCCATCATCCAGT
TGGCCAACGGGGAGCTAAAGAAGGTGGAAGACTTAAAAACAGAAGATTTC
ATCCAGAGTGCAGAGATAAGCAACGACCTGAAGATCGACTCCAGCACCGT
AGAGAGGATTGAAGACAGCCATAGCCCGGGCGTGGCCGTGATACAGTTCG
CCGTCGGGGAGCACCGAGCCCAGGTCAGCGTTGAAGTTTTGGTAGAGTAT
CCTTTTTTTGTGTTTGGACAGGGCTGGTCATCCTGCTGTCCGGAGAGAAC
CAGCCAGCTCTTTGATTTGCCGTGTTCCAAACTCTCAGTTGGGGATGTCT
GCATCTCGCTTACCCTCAAGAACCTGAAGAACGGCTCTGTTAAAAAGGGC
CAGCCCGTGGATCCCGCCAGCGTCCTGCTGAAGCACTCAAAGGCCGACGG
CCTGGCGGGCAGCAGACACAGGTATGCCGAGCAGGAAAACGGAATCAACC
AGGGGAGTGCCCAGATGCTCTCTGAGAATGGCGAACTGAAGTTTCCAGAG
AAAATGGGATTGCCTGCAGCGCCCTTCCTCACCAAAATAGAACCCAGCAA
GCCCGCGGCAACGAGGAAGAGGAGGTGGTCGGCGCCAGAGAGCCGCAAAC
TGGAGAAGTCAGAAGACGAACCACCTTTGACTCTTCCTAAGCCTTCTCTA
ATTCCTCAGGAGGTTAAGATTTGCATTGAAGGCCGGTCTAATGTAGGCAA
GTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKSNQE
RSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHG
GGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAY
ATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVT
SAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQ
QQHQHQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQN
TGRTASPPAIPVHLHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREAT
KKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPY
ESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREAS
PSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAG
TQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAP
AIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPV
VQSVASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISN
DLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQG
WSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASV
LLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAP
FLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKIC
IEGRSNVGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16999
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee