cDNA Clone slit-robo-GAP 2

• Plasmid: pcDNA5-FRT/TO-FLAG srGAP2a M1-T723
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG srGAP2a 1-723
• Genbank: NM_015326*
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATAGC
AGAGTACGATACTCAGGTCAAAGAGATCCGTGCTCAGCTCACAGAGCAGA
TGAAATGCCTGGACCAGCAGTGTGAGCTTCGGGTGCAACTGTTGCAGGAC
CTCCAGGACTTCTTCCGAAAGAAGGCAGAGATTGAGATGGACTACTCCCG
CAACCTGGAGAAGCTGGCAGAACGCTTCCTGGCCAAGACACGCAGCACCA
AGGACCAGCAATTCAAGAAGGATCAGAATGTTCTCTCTCCAGTCAACTGC
TGGAATCTCCTCTTAAACCAGGTGAAGCGGGAAAGCAGGGACCATACCAC
CCTGAGTGACATCTACCTGAATAATATCATTCCTCGATTTGTACAAGTCA
GCGAGGACTCAGGAAGACTCTTTAAAAAGAGTAAAGAAGTCGGCCAGCAG
CTCCAAGATGATTTGATGAAGGTCCTGAACGAGCTCTACTCGGTCATGAA
GACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTAA
AGGAGGCGGAGAAGCAGGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAG
GAGGACCGGCAGACCCCACGCTCCCCTGACTCCACGGCCAATGTTCGCAT
TGAGGAGAAACATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGA
AGGAGAAGCGCCAAGCCAAGTACACGGAGAATAAGCTGAAGGCCATCAAA
GCCCGGAATGAGTACTTGCTGGCTTTGGAGGCAACCAATGCATCTGTCTT
CAAGTACTACATCCATGACCTATCTGACCTTATTGATCAGTGTTGTGACT
TAGGCTACCATGCAAGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCT
GAGTTAAACCTGGAACAGTCGAAGCATGAGGGTCTGGATGCCATCGAGAA
TGCAGTAGAAAACCTGGATGCCACCAGTGACAAGCAGCGCCTCATGGAGA
TGTACAACAACGTCTTCTGCCCCCCTATGAAGTTTGAGTTTCAGCCCCAC
ATGGGGGATATGGCTTCCCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGA
GCTGGTACAGAGATGCCAACAACTGCAGTCTCGCTTATCCACTCTAAAGA
TTGAAAACGAAGAGGTAAAGAAGACAATGGAGGCCACCCTGCAAACCATC
CAGGACATTGTGACTGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTA
CAGCAACTCCATGGAGTCCGTCAAGTCCACGGTCTCTGAAACCTTCATGA
GCAAGCCCAGCATTGCTAAGAGGAGAGCCAACCAGCAAGAGACAGAGCAG
TTTTATTTCACAAAAATGAAAGAGTACCTGGAGGGCAGGAACCTCATCAC
CAAGTTACAAGCCAAGCATGACCTTCTGCAGAAAACCCTGGGAGAAAGTC
AGCGGACAGATTGCAGTCTAGCCAGGCGCAGCTCAACTGTGAGGAAACAG
GACTCCAGCCAGGCAATTCCTCTAGTGGTGGAAAGCTGTATCCGGTTTAT
CAGCAGACACGGACTACAGCATGAAGGAATTTTCCGGGTGTCAGGATCCC
AGGTGGAAGTGAATGACATCAAAAATGCCTTTGAGAGAGGAGAGGACCCC
CTGGCTGGGGACCAGAACGACCATGACATGGATTCCATAGCTGGTGTCCT
GAAGCTTTACTTCCGGGGGCTGGAACACCCTCTCTTCCCCAAGGACATCT
TTCATGACCTGATGGCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCT
CTGCACATCCGGAAAGTCCTCCTAGTCCTGCCCAAGACCACTCTGATTAT
CATGAGATACCTCTTTGCCTTCCTCAATCATTTATCACAGTTCAGTGAAG
AGAACATGATGGACCCCTACAACCTCGCCATCTGCTTCGGGCCCTCGCTA
ATGTCAGTGCCAGAGGGCCACGACCAGGTGTCCTGCCAAGCCCACGTGAA
TGAGCTGATCAAAACCATCATCATCCAGCATGAGAACATCTTCCCAAGCC
CCAGGGAGCTGGAGGGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGAT
TACTGTGATAGCCCTCATGGAGAGACTACCTCGGTTGAAGACTCAACCCA
GGATGTGACCGCAGAGCACCACACGTGACGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQC
ELRVQLLQDLQDFFRKKAEIEMDYSRNLEKLAERFLAKTRSTKDQQFKKD
QNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPRFVQVSEDSGRLF
KKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSKLKEAEKQEE
KQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKY
TENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCCDLGYHASLN
RALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCP
PMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRLSTLKIENEEVKK
TMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVSETFMSKPSIAKR
RANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGESQRTDCSLA
RRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIK
NAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLMACV
TMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLSQFSEENMMDPYN
LAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPSPRELEGPV
YSRGGSMEDYCDSPHGETTSVEDSTQDVTAEHHT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16993
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015326 CDS: g489c, c636t, t705c, g1518a, a1830g - all silent.