cDNA Clone slit-robo-GAP 1

• Plasmid: pcDNA5-FRT/TO-FLAG slit-robo-GAP 1 isoform 2 M1-T715
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG srGAP1-2 M1-T715
• Genbank: AB037725*
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGC
CGAGTATGAAAGTCAAGTCAAAGAAATTCGAGCTCAACTGGTAGAACAAC
AAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCAGGAT
CTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCG
GAATCTAGAGAAGTTAGCAGAAAGGTTCATGGCAAAAACAAGAAGCACTA
AGGATCATCAACAATACAAGAAAGACCAGAACCTGTTGTCTCCAGTGAAC
TGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGC
AACCTTGAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGA
TAAGTGAGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTC
CAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGAT
GAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGC
TGAAAGAGGCCGAAAAACAAGAGGAAAAGCAAATTGGGAGATCTGGTGAT
CCAGTCTTCCATATTCGACTAGAGGAGAGACATCAACGGCGAAGCTCTGT
AAAGAAAATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAA
ATAAGCTAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAA
GCCACCAATGCCTCAGTTTTCAAGTACTATATTCATGATCTTTCTGATTT
AATTGATTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCCCTAA
GAACATATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGC
TTAGACATTATTGAGAATGCAGTTGATAATTTAGAGCCCAGGAGCGATAA
GCAGAGATTCATGGAGATGTACCCTGCTGCGTTCTGTCCACCAATGAAGT
TTGAGTTTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAG
CAGCCAGTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCG
CCTTGCCACGCTCAAAATCGAGAATGAAGAGGTTAAGAAAACGACTGAAG
CCACCTTGCAGACGATACAAGATATGGTCACCATCGAGGACTATGATGTT
TCTGAATGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGT
CTCTGAAACCTACCTGAGTAAACCCAGCATCGCCAAGAGAAGAGCCAACC
AGCAGGAAACTGAACAGTTCTACTTCATGAAACTCAGAGAATATTTGGAA
GGCAGTAATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAG
GACCCTGGGAGAAGGTCATAGAGCTGAATATATGACTACAAGCCGAGGAC
GAAGAAACTCGCACACAAGACATCAGGACTCAGGACAGGTTATTCCCCTC
ATTGTGGAAAGCTGTATTCGGTTCATCAATCTCTATGGTCTTCAGCATCA
GGGGATTTTCAGAGTGTCTGGTTCCCAGGTGGAAGTCAATGATATTAAAA
ATTCATTTGAGAGAGGTGAAAATCCTTTGGCTGATGACCAGAGTAACCAT
GATATTAACTCAGTTGCTGGCGTTCTGAAGCTCTATTTCCGTGGGCTGGA
AAACCCCCTCTTTCCTAAGGAAAGATTTAACGATCTGATTTCTTGTATCA
GAATAGATAATCTCTATGAGAGGGCGCTTCACATCCGCAAACTCCTCCTG
ACTTTGCCCAGGTCGGTCCTTATAGTGATGAGGTACCTCTTTGCCTTCCT
CAATCATCTATCACAGTACAGCGATGAGAATATGATGGACCCTTATAACC
TGGCCATTTGCTTTGGCCCAACATTGATGCCTGTCCCAGAAATACAGGAT
CAAGTGTCTTGCCAGGCACATGTGAATGAAATTATCAAAACCATCATCAT
CCACCATGAGACTATTTTCCCAGATGCTAAAGAGCTGGATGGCCCTGTTT
ATGAGAAATGTATGGCTGGAGATGACTATTGCGACAGCCCATACAGTGAG
CACGGTACATTGGAGGAAGTGGACCAAGATGCTGGTACAGAGCCCCACAC
ATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQT
EMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYKK
DQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRM
FKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQE
EKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKA
RNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEY
NLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMG
DEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQD
MVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFY
FMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTSRGRRNSHTRH
QDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGEN
PLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYER
ALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPT
LMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKELDGPVYEKCMAGD
DYCDSPYSEHGTLEEVDQDAGTEPHT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16972
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. This clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…