cDNA Clone MAST2

• Plasmid: pFastbac GST MAST2
• Parent Plasmid: pFastbac GST
• Expressed: GST-MAST2
• Genbank: AB047005.1 GI:13537203 (BAB40778)
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGAAGCGGAGCCGCTGCCGCGACCGACCGCAGCCGCCGCCGCC
CGACCGCCGGGAGGATGGAGTTCAGCGGGCAGCGGAGCTGTCTCAGTCTT
TGCCGCCGCGCCGGCGAGCGCCGCCCGGGAGGCAGCGGCTGGAGGAGCGG
ACGGGCCCCGCGGGGCCCGAGGGCAAGGAGCAGGATGTAGTAACTGGAGT
TAGTCCCCTGCTCTTCAGGAAACTCAGTAATCCTGACATATTTTCATCCA
CTGGAAAAGTTAAACTTCAGCGACAACTGAGTCAGGATGATTGTAAGTTA
TGGAGAGGAAACCTGGCCAGCTCTCTATCGGGTAAGCAGCTGCTCCCTTT
GTCCAGCAGTGTACATAGCAGTGTGGGACAGGTGACTTGGCAGTCGTCAG
GAGAAGCATCAAACCTGGTTCGAATGAGAAACCAGTCCCTTGGACAGTCT
GCACCTTCTCTTACTGCTGGCCTGAAGGAGTTGAGCCTTCCAAGAAGAGG
CAGCTTTTGTCGGACAAGTAACCGCAAGAGCTTGATTGTGACCTCTAGCA
CATCACCTACACTACCACGGCCACACTCACCACTCCATGGCCACACAGGT
AACAGTCCTTTGGACAGCCCCCGGAATTTCTCTCCAAATGCACCTGCTCA
CTTTTCTTTTGTTCCTGCCCGTAGGACTGATGGGCGGCGCTGGTCTTTGG
CCTCTTTGCCCTCTTCAGGATATGGAACTAACACTCCTAGCTCCACTGTC
TCATCATCATGCTCCTCACAGGAAAAGCTGCATCAGTTGCCTTTCCAGCC
TACAGCTGATGAGCTGCACTTTTTGACGAAGCATTTCAGCACAGAGAGCG
TACCAGATGAGGAAGGACGGCAGTCCCCAGCCATGCGGCCTCGCTCCCGG
AGCCTCAGTCCCGGACGATCCCCAGTATCCTTTGACAGTGAAATAATAAT
GATGAATCACGTTTACAAAGAAAGATTCCCAAAGGCCACCGCACAAATGG
AAGAGCGACTAGCAGAGTTTATTTCCTCCAACACTCCAGACAGCGTGCTG
CCCTTGGCAGATGGAGCCCTGAGCTTTATTCATCATCAGGTGATTGAGAT
GGCCCGAGACTGCCTGGATAAATCTCGGAGTGGCCTCATTACATCACAAT
ACTTCTACGAACTTCAAGAGAATTTGGAGAAACTTTTACAAGATGCTCAT
GAGCGCTCAGAGAGCTCAGAAGTGGCTTTTGTGATGCAGCTGGTGAAAAA
GCTGATGATTATCATTGCCCGCCCAGCACGTCTCCTGGAATGCCTGGAGT
TTGACCCTGAAGAGTTCTACCACCTTTTAGAAGCAGCTGAGGGCCACGCC
AAAGAGGGACAAGGGATTAAATGTGACATTCCCCGCTACATCGTTAGCCA
GCTGGGCCTCACCCGGGATCCCCTAGAAGAAATGGCCCAGTTGAGCAGCT
GTGACAGTCCTGACACTCCAGAGACAGATGATTCTATTGAGGGCCATGGG
GCATCTCTGCCATCTAAAAAGACACCCTCTGAAGAGGACTTCGAGACCAT
TAAGCTCATCAGCAATGGCGCCTATGGGGCTGTATTTCTGGTGCGGCACA
AGTCCACCCGGCAGCGCTTTGCCATGAAGAAGATCAACAAGCAGAACCTG
ATCCTACGGAACCAGATCCAGCAGGCCTTCGTGGAGCGTGACATACTGAC
TTTCGCTGAGAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGATACCA
AGCGCCACTTGTGCATGGTGATGGAGTACGTTGAAGGGGGAGACTGTGCC
ACTCTGCTGAAGAATATTGGGGCCCTGCCTGTGGACATGGTGCGTCTATA
CTTTGCGGAAACTGTGCTGGCCCTGGAGTACTTACACAACTATGGCATCG
TGCACCGTGACCTCAAGCCTGACAACCTCCTAATTACATCCATGGGGCAC
ATCAAGCTCACGGACTTTGGACTGTCCAAAATGGGCCTCATGAGTCTGAC
AACGAACTTGTATGAGGGTCATATTGAAAAGGATGCCCGGGAATTCCTGG
ACAAGCAGGTATGCGGGACCCCAGAATACATTGCGCCTGAGGTGATCCTG
CGCCAGGGCTATGGGAAGCCAGTGGACTGGTGGGCCATGGGCATTATCCT
GTATGAGTTCCTGGTGGGCTGCGTCCCTTTTTTTGGAGATACTCCGGAGG
AGCTCTTTGGGCAGGTGATCAGTGATGAGATTGTGTGGCCTGAGGGTGAT
GAGGCACTGCCCCCAGACGCCCAGGACCTCACCTCCAAACTGCTCCACCA
GAACCCTCTGGAGAGACTTGGCACAGGCAGTGCCTATGAGGTGAAGCAGC
ACCCATTCTTTACTGGTCTGGACTGGACAGGACTTCTCCGCCAGAAGGCT
GAATTTATTCCTCAGTTGGAGTCAGAGGATGATACTAGCTATTTTGACAC
CCGCTCAGAGCGATACCACCACATGGACTCGGAGGATGAGGAAGAAGTGA
GTGAGGATGGCTGCCTTGAGATCCGCCAGTTCTCTTCCTGCTCTCCAAGG
TTCAACAAGGTGTACAGCAGCATGGAGCGGCTCTCACTGCTCGAGGAGCG
CCGGACACCACCCCCGACCAAGCGCAGCCTGAGTGAGGAGAAGGAGGACC
ATTCAGATGGCCTGGCAGGGCTCAAAGGCCGAGACCGGAGCTGGGTGATT
GGCTCCCCTGAGATATTACGGAAGCGGCTGTCGGTGTCTGAGTCGTCCCA
CACAGAGAGTGACTCAAGCCCTCCAATGACAGTGCGACGCCGCTGCTCAG
GCCTCCTGGATGCGCCTCGGTTCCCGGAGGGCCCTGAGGAGGCCAGCAGC
ACCCTCAGGAGGCAACCACAGGAGGGTATATGGGTCCTGACACCCCCATC
TGGAGAGGGGGTATCTGGGCCTGTCACTGAACACTCAGGGGAGCAGCGGC
CAAAGCTGGATGAGGAAGCTGTTGGCCGGAGCAGTGGTTCCAGTCCAGCT
ATGGAGACCCGAGGCCGTGGGACCTCACAGCTGGCTGAGGGAGCCACAGC
CAAGGCCATCAGTGACCTGGCTGTGCGTAGGGCCCGCCACCGGCTGCTCT
CTGGGGACTCAACAGAGAAGCGCACTGCTCGCCCTGTCAACAAAGTGATC
AAGTCCGCCTCAGCCACAGCCCTCTCACTCCTCATTCCTTCGGAACACCA
CACCTGCTCCCCGTTGGCCAGCCCCATGTCCCCACATTCTCAGTCGTCCA
ACCCATCATCCCGGGACTCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTT
GGCAGCATGAGGCCTCCCATCATCATCCACCGAGCTGGCAAGAAGTATGG
CTTCACCCTGCGGGCCATTCGCGTCTACATGGGTGACTCCGATGTCTACA
CCGTGCACCATATGGTGTGGCACGTGGAGGATGGAGGTCCGGCCAGTGAG
GCAGGGCTTCGTCAAGGTGACCTCATCACCCATGTCAATGGGGAACCTGT
GCATGGCCTGGTGCACACGGAGGTGGTGGAGCTGATCCTGAAGAGTGGAA
ACAAGGTGGCCATTTCAACAACTCCCCTGGAGAACACATCCATTAAAGTG
GGGCCAGCTCGGAAGGGCAGCTACAAGGCCAAGATGGCCCGAAGGAGCAA
GAGGAGCCGCGGCAAGGATGGGCAAGAAAGCAGAAAAAGGAGCTCCCTGT
TCCGCAAGATCACCAAGCAAGCATCCCTGCTCCACACCAGCCGCAGCCTT
TCTTCCCTTAACCGCTCCTTGTCATCAGGGGAGAGTGGGCCAGGCTCTCC
CACACACAGCCACAGCCTTTCCCCCCGATCTCCCACTCAAGGCTACCGGG
TGACCCCCGATGCTGTGCATTCAGTGGGAGGGAATTCATCACAGAGCAGC
TCCCCCAGCTCCAGCGTGCCCAGTTCCCCAGCCGGCTCTGGGCACACACG
GCCCAGCTCCCTCCACGGTCTGGCACCCAAGCTCCAACGCCAGTACCGCT
CTCCACGGCGCAAGTCAGCAGGCAGCATCCCACTGTCACCACTGGCCCAC
ACCCCTTCTCCCCCACCCCCAACAGCTTCACCTCAGCGGTCCCCATCGCC
CCTGTCTGGCCATGTAGCCCAGGCCTTTCCCACAAAGCTTCACTTGTCAC
CTCCCCTGGGCAGGCAACTCTCACGGCCCAAGAGTGCGGAGCCACCCCGT
TCACCACTACTCAAGAGGGTGCAGTCGGCTGAGAAACTGGCAGCAGCACT
TGCCGCCTCTGAGAAGAAGCTAGCCACTTCTCGCAAGCACAGCCTTGACC
TGCCCCACTCTGAACTAAAGAAGGAACTGCCGCCCAGGGAAGTGAGCCCT
CTGGAGGTAGTTGGAGCCAGGAGTGTGCTGTCTGGCAAGGGGGCCCTGCC
AGGGAAGGGGGTGCTGCAGCCTGCTCCCTCACGGGCCCTAGGCACCCTCC
GGCAGGACCGAGCCGAACGACGGGAGTCGCTGCAGAAGCAAGAAGCCATT
CGTGAGGTGGACTCCTCAGAGGACGACACCGAGGAAGGGCCTGAGAACAG
CCAGGGTGCACAGGAGCTGAGCTTGGCACCTCACCCAGAAGTGAGCCAGA
GTGTGGCCCCTAAAGGAGCAGGAGAGAGTGGGGAAGAGGATCCTTTCCCG
TCCAGAGGCCCTAGGAGCCTGGGCCCAATGGTCCCAAGCCTATTGACAGG
GATCACACTGGGGCCTCCCAGAATGGAAAGTCCCAGTGGTCCCCACAGGA
GGCTCGGGAGCCCACAAGCCATTGAGGAGGCTGCCAGCTCCTCCTCAGCA
GGCCCCAACCTAGGTCAGTCTGGAGCCACAGACCCCATCCCTCCTGAAGG
TTGCTGGAAGGCCCAGCACCTCCACACCCAGGCACTAACAGCACTTTCTC
CCAGCACTTCGGGACTCACCCCCACCAGCAGTTGCTCTCCTCCCAGCTCC
ACCTCTGGGAAGCTGAGCATGTGGTCCTGGAAATCCCTTATTGAGGGCCC
AGACAGGGCATCCCCAAGCAGAAAGGCAACCATGGCAGGTGGGCTAGCCA
ACCTCCAGGATTTGGAAACACAACTCCAGCCCAGCCTAAGAACCTGTCTC
CCAGGGAGCAGGGGAAGACACAGCCACCTAGTGCCCCCAGACTGGCCCAT
CCATCTTATGAGGATCCCAGCCAGGGCTGGCTATGGGAGTCTGAGTGTGC
ACAAGCAGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKRSRCRDRPQPPPPDRRE
DGVQRAAELSQSLPPRRRAPPGRQRLEERTGPAGPEGKEQDVVTGVSPLL
FRKLSNPDIFSSTGKVKLQRQLSQDDCKLWRGNLASSLSGKQLLPLSSSV
HSSVGQVTWQSSGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCR
TSNRKSLIVTSSTSPTLPRPHSPLHGHTGNSPLDSPRNFSPNAPAHFSFV
PARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPFQPTADE
LHFLTKHFSTESVPDEEGRQSPAMRPRSRSLSPGRSPVSFDSEIIMMNHV
YKERFPKATAQMEERLAEFISSNTPDSVLPLADGALSFIHHQVIEMARDC
LDKSRSGLITSQYFYELQENLEKLLQDAHERSESSEVAFVMQLVKKLMII
IARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKCDIPRYIVSQLGLT
RDPLEEMAQLSSCDSPDTPETDDSIEGHGASLPSKKTPSEEDFETIKLIS
NGAYGAVFLVRHKSTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAEN
PFVVSMFCSFDTKRHLCMVMEYVEGGDCATLLKNIGALPVDMVRLYFAET
VLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLY
EGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFL
VGCVPFFGDTPEELFGQVISDEIVWPEGDEALPPDAQDLTSKLLHQNPLE
RLGTGSAYEVKQHPFFTGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSER
YHHMDSEDEEEVSEDGCLEIRQFSSCSPRFNKVYSSMERLSLLEERRTPP
PTKRSLSEEKEDHSDGLAGLKGRDRSWVIGSPEILRKRLSVSESSHTESD
SSPPMTVRRRCSGLLDAPRFPEGPEEASSTLRRQPQEGIWVLTPPSGEGV
SGPVTEHSGEQRPKLDEEAVGRSSGSSPAMETRGRGTSQLAEGATAKAIS
DLAVRRARHRLLSGDSTEKRTARPVNKVIKSASATALSLLIPSEHHTCSP
LASPMSPHSQSSNPSSRDSSPSRDFLPALGSMRPPIIIHRAGKKYGFTLR
AIRVYMGDSDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLV
HTEVVELILKSGNKVAISTTPLENTSIKVGPARKGSYKAKMARRSKRSRG
KDGQESRKRSSLFRKITKQASLLHTSRSLSSLNRSLSSGESGPGSPTHSH
SLSPRSPTQGYRVTPDAVHSVGGNSSQSSSPSSSVPSSPAGSGHTRPSSL
HGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPPPTASPQRSPSPLSGH
VAQAFPTKLHLSPPLGRQLSRPKSAEPPRSPLLKRVQSAEKLAAALAASE
KKLATSRKHSLDLPHSELKKELPPREVSPLEVVGARSVLSGKGALPGKGV
LQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQ
ELSLAPHPEVSQSVAPKGAGESGEEDPFPSRGPRSLGPMVPSLLTGITLG
PPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKA
QHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRAS
PSRKATMAGGLANLQDLETQLQPSLRTCLPGSRGRHSHLVPPDWPIHLMR
IPARAGYGSLSVHKQ*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU16915
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 216254 Daltons