cDNA Clone RFX2

• Plasmid: pcDNA5-FRT/TO-GFP RFX2
• Parent Plasmid: pcDNA5 FRT/TO GFPTAP
• Expressed: GFP RFX2
• Genbank: NM_022841.5 GI:148613885
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccatggcagaggaacaacaacagccgccaccacagcagcctgatgc
ccatcagcagcttccccccagcgcccccaactcgggggtggccctgccag
cccttgtgcccgggctgccagggacagaggccagcgcgctgcaacacaag
atcaagaactccatctgcaaaactgtacaatctaaagtggactgcatttt
gcaagaagttgagaagtttacagacctagagaaactctacctctaccttc
agctgccttctggtctcagcaatggagagaaaagtgatcagaatgccatg
tcatctagtcgggcacaacaaatgcatgccttttcctggattcggaatac
cctagaggaacatccggagacttcactgcccaaacaggaagtctatgatg
agtacaagagctattgtgacaatcttggttaccatccattaagtgctgct
gattttggaaagatcatgaaaaacgtctttccaaacatgaaggcacgtcg
tttgggcacaagaggcaaatctaaatattgctacagtggactaagaaaaa
aagcttttgttcatatgccaacactgcccaaccttgactttcacaaaact
ggagatgggttggaaggagctgaaccttctgggcagcttcaaaatattga
tgaagaagttatctcttctgcttgccgtcttgtgtgtgagtgggcccaga
aagtgttaagccaaccatttgacaccgtcttggaattagcccgcttcctt
gtaaaaagtcactatataggcaccaagtcaatggcagctctaactgtaat
ggcagcagcaccagcaggaatgaaaggaattacccagccttctgctttta
tacctacagctgaaagtaattcctttcagcctcaggtgaagactttgcca
tctccaattgatgctaaacagcagttgcaacggaaaatccagaagaagca
gcaagaacagaaactacaatcccctttgccaggagaatctgcagcaaaaa
agtcagaaagtgctacaagcaatggagtgactaatcttcctaatggaaat
ccttcaatcctttctcctcaacctattggtatcgttgtggcagctgtccc
tagtcccattccggtccagcggactaggcaattggtaacttcaccgagtc
caatgagttcttctgacggcaaagttcttcccctcaatgtacaggtggtc
actcagcacatgcagtctgtgaaacaggcaccaaagactccccagaacgt
tccagccagtcctggtggggatcgttctgcccggcaccgttaccctcaga
tcttacccaaaccagcgaacaccagtgcactcaccattcgctctccaact
actgtcctctttactagtagtcccatcaaaactgctgttgtacccgcttc
acacatgagttctctaaatgtggtgaaaatgacaacaatatccctcacac
ccagcaacagtaacacccctcttaaacattctgcctcagtcagcagtgct
acaggaacaacagaagaatcaaggagtgttccacagatcaagaatggttc
tgtcgtgtcgcttcagtctcctgggtccaggagcagcagtgcggggggaa
catctgctgtggaagtcaaagtggaacccgaaacatcatcagatgagcat
cctgtacagtgccaagagaactctgatgaggctaaagctccccagacacc
tagtgcccttttggggcagaaaagtaatacagacggagcactgcagaaac
cttcaaatgaaggtgtcattgaaataaaagcaactaaggtctgtgaccag
aggaccaaatgtaaaagtcgctgtaatgaaatgctgccaggcacgtcaac
aggcaataatcaaagcactatcactctatcagttgcttctcagaacttaa
ctttcaccagcagcagctcaccacctaatggtgactcaatcaataaagac
cctaaattatgcactaaaagcccaagaaaacgactgtcttctacattgca
agagacccaggtgcctcctgtaaagaaaccaattgtggaacagctttcag
cagctaccatagaagggcagaaacaaggcagtgttaagaaggaccaaaag
gttccacattcagggaaaacagaaggttcaacagcaggtgctcagattcc
tagcaaggtatcagtaaatgtcagttcacacataggagcaaatcaaccct
tgaattcctctgcccttgttatcagtgattcagctttggaacagcaaaca
accccatcatcatctccagatataaaagtaaaacttgaaggaagtgtctt
tctcttggacagtgattcaaagtcagttggcagctttaatccaaatggat
ggcaacaaatcactaaagattctgagtttatatctgccagttgtgaacaa
cagcaagatatcagtgttatgacaattcctgagcactctgatatcaatga
cttagagaaatctgtttgggaattagaaggaatgccacaggacacatata
gccagcagctacatagccagatacaggaatcttctttaaatcaaatacaa
gcacattcttcagatcagttacctctgcaatctgaactgaaggagtttga
gccttctgtttcccagacaaatgaaagctactttccttttgatgatgaac
ttacacaagatagtattgtggaagagctggtgcttatggagcagcaaatg
tcaatgaacaattctcattcttacggcaactgtttgggaatgacccttca
gagtcagtcagtaactccaggagctccaatgtcatctcacacttccagca
cccacttctatcatccaatccacagcaatggcactccaatccacacaccc
acacccacacccacacccactcctactccaaccccaaccccaaccccgac
atctgaaatgattgctggatctcagagtctgtcacgggagagcccttgct
ccaggctagcccagactacacctgtggatagtgctttaggaagtagccga
catacacccattggtactccacattctaactgcagcagtagtgtcccccc
cagccctgttgaatgcaggaatccgtttgcattcactccaataagctcca
gtatggcatatcatgacgccagcattgtctcaagtagtcctgtgaaaccg
atgcaaagacccatggccacacaccctgacaaaaccaagcttgaatggat
gaataatgggtatagtggggttggtaattcatcagtttctggccatggta
ttctcccaagctatcaggaactagtggaagaccgtttcaggaaacctcat
gcttttgctgtgcctggacagtcttatcagtctcaatccagacatcatga
cactaattttggtcgtttgactcctgtctctcctgtgcagcatcaaggtg
ccactgtaaataacaccaacaaacaggagggttttgcagtccctgcccct
cttgataataaaggaactaattcatctgccagcagcaacttcagatgccg
gagtgtgagccctgctgttcatcgccaacgtaatcttagtggaagcaccc
tctatccagtatctaatatcccacgatctaatgtgaccccctttggaagt
ccagttaccccagaagttcatgttttcacaaatgttcacacagacgcatg
tgccaacaacatagctcaaagaagccaatcagttccattgacagtcatga
tgcagacagccttcccaaacgctcttcagaagcaagcaaacagtaaaaaa
ataaccaatgttttgttgagtaaacttgattccgacaatgatgatgcagt
gagaggtttgggaatgaacaacctgccctctaattatacagcccggatga
atctcactcagattttggaaccttccactgtttttcctagtgccaaccca
caaaatatgatcgattccagcacttctgtttatgagttccaaacaccatc
ttacctcaccaaaagtaatagcaccggtcagatcaatttttctcctggag
ataatcaagcacaatcagaaattggagagcaacaattagatttcaatagc
actgttaaagacctgttgagtggagacagcttgcaaaccaaccagcagct
ggtaggtcagggagcatctgatctcactaatactgcatctgatttctcta
gcgatatcaggttgtcttctgagctctcaggcagcatcaatgatttgaac
actttagacccaaatctactgtttgatccaggtcgtcagcagggacaaga
tgatgaagctacactggaagaattaaagaatgacccattatttcaacaaa
tttgcagtgaatccatgaattctatgacttcatcaggttttgaatggata
gaaagcaaggaccatcctactgttgaaatgttgggttaagcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSMAEE
QQQPPPQQPDAHQQLPPSAPNSGVALPALVPGLPGTEASALQHKIKNSIC
KTVQSKVDCILQEVEKFTDLEKLYLYLQLPSGLSNGEKSDQNAMSSSRAQ
QMHAFSWIRNTLEEHPETSLPKQEVYDEYKSYCDNLGYHPLSAADFGKIM
KNVFPNMKARRLGTRGKSKYCYSGLRKKAFVHMPTLPNLDFHKTGDGLEG
AEPSGQLQNIDEEVISSACRLVCEWAQKVLSQPFDTVLELARFLVKSHYI
GTKSMAALTVMAAAPAGMKGITQPSAFIPTAESNSFQPQVKTLPSPIDAK
QQLQRKIQKKQQEQKLQSPLPGESAAKKSESATSNGVTNLPNGNPSILSP
QPIGIVVAAVPSPIPVQRTRQLVTSPSPMSSSDGKVLPLNVQVVTQHMQS
VKQAPKTPQNVPASPGGDRSARHRYPQILPKPANTSALTIRSPTTVLFTS
SPIKTAVVPASHMSSLNVVKMTTISLTPSNSNTPLKHSASVSSATGTTEE
SRSVPQIKNGSVVSLQSPGSRSSSAGGTSAVEVKVEPETSSDEHPVQCQE
NSDEAKAPQTPSALLGQKSNTDGALQKPSNEGVIEIKATKVCDQRTKCKS
RCNEMLPGTSTGNNQSTITLSVASQNLTFTSSSSPPNGDSINKDPKLCTK
SPRKRLSSTLQETQVPPVKKPIVEQLSAATIEGQKQGSVKKDQKVPHSGK
TEGSTAGAQIPSKVSVNVSSHIGANQPLNSSALVISDSALEQQTTPSSSP
DIKVKLEGSVFLLDSDSKSVGSFNPNGWQQITKDSEFISASCEQQQDISV
MTIPEHSDINDLEKSVWELEGMPQDTYSQQLHSQIQESSLNQIQAHSSDQ
LPLQSELKEFEPSVSQTNESYFPFDDELTQDSIVEELVLMEQQMSMNNSH
SYGNCLGMTLQSQSVTPGAPMSSHTSSTHFYHPIHSNGTPIHTPTPTPTP
TPTPTPTPTPTSEMIAGSQSLSRESPCSRLAQTTPVDSALGSSRHTPIGT
PHSNCSSSVPPSPVECRNPFAFTPISSSMAYHDASIVSSSPVKPMQRPMA
THPDKTKLEWMNNGYSGVGNSSVSGHGILPSYQELVEDRFRKPHAFAVPG
QSYQSQSRHHDTNFGRLTPVSPVQHQGATVNNTNKQEGFAVPAPLDNKGT
NSSASSNFRCRSVSPAVHRQRNLSGSTLYPVSNIPRSNVTPFGSPVTPEV
HVFTNVHTDACANNIAQRSQSVPLTVMMQTAFPNALQKQANSKKITNVLL
SKLDSDNDDAVRGLGMNNLPSNYTARMNLTQILEPSTVFPSANPQNMIDS
STSVYEFQTPSYLTKSNSTGQINFSPGDNQAQSEIGEQQLDFNSTVKDLL
SGDSLQTNQQLVGQGASDLTNTASDFSSDIRLSSELSGSINDLNTLDPNL
LFDPGRQQGQDDEATLEELKNDPLFQQICSESMNSMTSSGFEWIESKDHP
TVEMLG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16662
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee