cDNA Clone BTBD12

• Synonyms: SLX4
• Plasmid: pEG(KT) BTBD12
• Parent Plasmid: pEG(KT)
• Expressed: GST-BTBD12
• Genbank: NM_032444.2 GI:63252862*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAACTGAGTGTGAATGAGGCTCAGCTAGGCTTCTACTTGGG
TTCACTTTCTCATCTGTCTGCCTGTCCTGGGATTGACCCTCGCTCCTCTG
AAGACCAGCCTGAAAGCCTTAAAACTGGTCAGATGATGGATGAGTCTGAT
GAGGACTTTAAAGAACTCTGCGCTAGCTTTTTCCAAAGGGTGAAAAAACA
TGGAATCAAGGAAGTGTCAGGAGAAAGGAAGACACAAAAGGCTGCCTCAA
ACGGCACTCAGATAAGAAGCAAATTGAAAAGGACCAAACAAACTGCTACC
AAGACCAAAACCCTTCAAGGCCCTGCAGAGAAGAAACCTCCGTCTGGCAG
CCAGGCCCCTAGGACTAAAAAGCAAAGGGTAACCAAATGGCAAGCAAGTG
AACCGGCCCACTCTGTGAATGGGGAGGGGGGTGTGCTTGCCTCTGCTCCA
GATCCACCTGTGCTCCGGGAAACAGCACAAAACACCCAGACGGGTAACCA
GCAAGAACCATCGCCAAACCTTTCCAGAGAGAAAACCAGAGAGAATGTGC
CCAACAGCGACTCCCAGCCTCCTCCTTCCTGTTTGACAACAGCAGTGCCA
AGTCCCTCCAAACCCCGCACAGCACAATTGGTCCTACAGCGAATGCAGCA
GTTCAAGAGAGCAGACCCCGAGCGTTTGAGACACGCTTCAGAAGAGTGCT
CCCTCGAGGCTGCGCGGGAAGAAAATGTCCCAAAGGACCCTCAAGAGGAG
ATGATGGCGGGGAATGTGTATGGGCTTGGGCCCCCTGCCCCAGAGAGCGA
CGCTGCGGTGGCCTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCAT
CGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATT
TGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGT
GAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTC
AGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGC
AGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCA
GCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGCA
GCAGCCCACCCATGTTCAGCTTCAGTGATCACAGTAGAGGTCTGAAACGG
AGAGGACCCACCAGCAAGAAGGAGCCACGGAAGAGGCGGAAGGTGGACGA
GGCACCGTCCGAGGACCTGCTGGTGGCCATGGCTCTGTCCCGGTCGGAGA
TGGAGCCGGGTGCGGCTGTACCAGCGCTCAGGCTGGAAAGTGCCTTTTCT
GAGAGGATAAGACCAGAAGCAGAGAATAAAAGTCGCAAGAAGAAACCCCC
GGTATCCCCCCCATTGTTGTTAGTCCAGGACTCTGAAACCACAGGCCGAC
AGATAGAGGACCGTGTGGCCCTGCTCCTCTCTGAGGAAGTGGAATTGTCT
AGCACGCCACCACTTCCTGCCAGCAGGATTTTAAAGGAAGGGTGGGAAAG
AGCGGGCCAGTGTCCTCCTCCACCTGAACGCAAGCAGAGCTTTCTGTGGG
AGGGCAGCGCACTGACTGGGGCCTGGGCCATGGAGGACTTCTACACGGCC
AGGCTGGTCCCTCCTCTCGTGCCCCAGCGGCCTGCCCAGGGCCTTATGCA
GGAGCCCGTGCCGCCTCTGGTGCCACCTGAGCACTCAGAGCTGAGCGAGC
GAAGGTCACCCGCTCTCCACGGCACCCCCACTGCAGGCTGTGGCTCCAGG
GGCCCGTCGCCTTCGGCCAGCCAGAGGGAGCACCAGGCCCTGCAGGACCT
CGTGGACCTGGCGAGGGAGGGACTGAGCGCCAGCCCGTGGCCCGGCAGTG
GGGGCCTGGCTGGCTCGGAAGGGACTGCAGGGTTGGACGTGGTGCCCGGC
GGCCTTCCTCTGACTGGGTTTGTGGTGCCATCGCAGGACAAGCACCCGGA
CAGGGGCGGCCGAACCTTGCTCTCCCTCGGGCTGCTGGTTGCTGACTTTG
GCGCCATGGTCAATAACCCACACCTGAGTGATGTCCAGTTTCAGACGGAC
AGCGGGGAGGTGCTTTACGCCCACAAGTTCGTGCTTTATGCCCGATGCCC
GCTCCTCATCCAGTATGTGAACAATGAAGGCTTCTCCGCTGTAGAGGACG
GGGTTCTGACCCAGCGTGTCCTGCTGGGTGACGTGAGCACCGAGGCCGCC
CGCACGTTCCTGCACTATCTCTACACTGCGGACACTGGCCTTCCTCCTGG
CCTTAGCTCTGAGCTGAGCTCCCTGGCCCACAGGTTTGGCGTGAGTGAGC
TCGTTCACCTGTGCGAACAGGTGCCTATTGCCACTGACTCAGAGGGCAAA
CCATGGGAGGAGAAGGAAGCAGAGAATTGCGAAAGCAGGGCCGAGAATTT
CCAGGAACTCTTGAGGTCAATGTGGGCAGATGAAGAGGAGGAAGCGGAGA
CTTTGTTGAAATCCAAGGACCACGAAGAAGATCAAGAAAACGTGAATGAA
GCAGAAATGGAAGAAATTTATGAATTTGCAGCTACTCAGCGAAAGCTTCT
CCAGGAAGAAAGGGCAGCGGGTGCCGGCGAGGACGCTGACTGGCTGGAGG
GTGGCAGTCCGGTTTCTGGGCAACTCCTAGCAGGTGTCCAGGTGCAGAAA
CAGTGGGACAAGGTGGAGGAGATGGAGCCGTTGGAGCCAGGAAGAGATGA
GGCCGCCACCACCTGGGAGAAGATGGGACAGTGCGCTCTCCCGCCACCCC
AGGGCCAGCACTCAGGGGCACGGGGAGCAGAGGCCCCTGAGCAGGAGGCG
CCAGAGGAGGCGCTTGGCCATTCCAGCTGCTCCAGCCCTTCCAGGGACTG
CCAGGCAGAGAGAAAAGAAGGCTCTCTTCCGCACTCAGATGATGCCGGGG
ATTACGAACAGCTCTTCTCATCAACTCAGGGAGAGATCTCAGAGCCGTCC
CAAATAACAAGTGAGCCCGAGGAACAAAGTGGCGCTGTCAGGGAAAGGGG
GCTGGAGGTTTCTCATCGCCTGGCTCCCTGGCAGGCATCTCCACCGCACC
CGTGCCGCTTCCTATTGGGGCCTCCCCAGGGCGGGAGTCCCCGCGGGTCT
CATCACACAAGTGGGTCGTCCCTGTCAACACCCCGGTCCCGTGGCGGAAC
TTCCCAGGTGGGCTCCCCAACCTTGCTGTCTCCAGCTGTGCCATCAAAGC
AGAAAAGGGACAGGAGCATCCTCACGCTGTCTAAAGAGCCAGGGCACCAG
AAAGGCAAAGAGCGTCGGTCCGTGCTGGAGTGCAGAAATAAGGGGGTCCT
GATGTTCCCAGAAAAATCTCTGTCTATTGACCTAACCCAGTCAAATCCTG
ACCATTCGAGCTCCAGATCTCAGAAATCTTCATCCAAACTGAACGAAGAA
GATGAGGTCATCCTCTTACTGGACTCGGATGAGGAGCTGGAGCTAGAACA
AACCAAAATGAAGTCCATTTCTAGTGATCCTCTGGAAGAAAAGAAAGCTC
TAGAAATTAGCCCTAGGTCCTGTGAGCTGTTTTCCATCATTGATGTTGAT
GCAGATCAGGAACCTTCCCAGAGCCCACCAAGAAGCGAAGCTGTGCTGCA
GCAGGAGGATGAGGGGGCGCTGCCGGAGAATCGGGGCTCTTTGGGCAGGA
GAGGGGCTCCCTGGCTGTTCTGTGACCGTGAGAGCAGCCCCAGCGAGGCC
AGCACCACAGACACCTCGTGGCTGGTGCCCGCCACCCCGCTGGCCAGCAG
AAGCCGTGACTGTTCTTCCCAGACCCAAATCAGCAGCCTCAGGAGCGGGC
TGGCCGTGCAGGCGGTGACTCAGCACACGCCCAGGGCCTCAGTAGGAAAC
AGGGAAGGGAACGAAGTCGCACAGAAGTTTTCTGTCATCAGGCCCCAGAC
ACCACCGCCCCAGACACCGTCCTCATGCCTCACTCCCGTCTCTCCAGGAA
CTTCTGACGGCAGAAGGCAAGGCCACAGAAGCCCTTCCCGTCCCCACCCC
GGGGGCCACCCGCACTCCTCTCCGCTGGCTCCACATCCCATCTCAGGGGA
CCGCGCCCACTTCAGCAGGCGGTTCCTGAAACACTCGCCGCCTGGGCCAA
GCTTCCTGAACCAGACCCCAGCGGGTGAAGTGGTGGAAGTCGGAGACAGT
GACGATGAGCAGGAGGTGGCCTCCCATCAGGCCAACAGAAGCCCCCCACT
GGACAGTGACCCCCCAATTCCAATTGACGACTGCTGCTGGCACATGGAGC
CCCTCTCGCCAATTCCCATTGACCACTGGAACCTGGAGCGGACCGGCCCC
CTGAGCACCAGCAGCCCCAGCCGCAGGATGAACGAGGCCGCCGACAGCCG
TGACTGTCGCTCCCCGGGACTCCTGGACACCACCCCCATCCGAGGAAGCT
GCACTACCCAGAGGAAATTGCAAGAGAAGTCCTCGGGCGCGGGCTCCCTG
GGGAACAGCAGGCCGAGCTTTCTGAATTCGGCTCTGTGGGACGTTTGGGA
CGGGGAAGAGCAGAGGCCTCCAGAGACCCCTCCTCCGGCCCAGATGCCAA
GCGCTGGTGGAGCTCAGAAGCCCGAAGGGTTAGAGACACCCAAAGGTGCT
AATCGGAAGAAGAACTTGCCCCCCAAAGTGCCCATAACGCCGATGCCACA
GTATTCCATTATGGAGACGCCGGTGCTGAAGAAGGAACTGGATAGGTTTG
GAGTCCGCCCTCTGCCTAAACGCCAGATGGTTCTGAAGCTGAAGGAGATA
TTCCAGTACACTCACCAGACCCTGGACTCAGACTCCGAGGACGAGAGCCA
GTCCTCACAGCCGCTGTTGCAGGCGCCTCACTGCCAGACCCTCGCCTCCC
AGACCTACAAGCCTTCAAGGGCAGGGGTCCATGCCCAGCAGGAGGCCACC
ACAGGACCTGGGGCCCATAGGCCCAAGGGACCTGCTAAGACCAAGGGCCC
CCGACATCAAAGGAAGCATCATGAAAGCATCACACCCCCAAGCAGGTCGC
CCACCAAGGAGGCACCTCCAGGCCTCAATGATGACGCCCAGATCCCAGCC
TCTCAAGAATCCGTGGCCACCTCTGTGGATGGCAGTGACAGCTCCTTGAG
CTCACAGAGTTCTTCCTCCTGTGAGTTTGGAGCGGCATTTGAGTCTGCAG
GTGAAGAGGAGGGCGAGGGGGAGGTCAGTGCCTCGCAGGCAGCCGTGCAG
GCGGCGGACACAGACGAGGCGCTGAGGTGCTACATCCGCTCCAAGCCGGC
CCTGTACCAGAAGGTGCTGCTGTACCAGCCCTTTGAGCTGCGGGAGCTGC
AGGCAGAGCTGAGGCAGAACGGCCTCCGTGTGTCCTCGCGCAGGCTGTTG
GACTTCCTGGACACCCACTGTATCACCTTCACCACTGCCGCCACCCGCAG
GGAGAAGCTCCAGGGCAGGAGGCGGCAGCCTCGGGGCAAGAAGAAGGTGG
AGCGGAACTGAGTCGAC

Amino Acid Sequence

MKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDED
FKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKT
KTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDP
PVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSP
SKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMM
AGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQ
KNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRT
SHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRG
PTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSER
IRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSST
PPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARL
VPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGP
SPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGL
PLTGFVVPSQDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSDVQFQTDSG
EVLYAHKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVSTEAART
FLHYLYTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGKPW
EEKEAENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAE
MEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQW
DKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPE
EALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQI
TSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHH
TSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKG
KERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDE
VILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDAD
QEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEAST
TDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNRE
GNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGG
HPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDD
EQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLS
TSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGN
SRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANR
KKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQ
YTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTG
PGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQ
ESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAA
DTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDF
LDTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU16476
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector with N-terminal GST tag.; ; Sequence difference compared with NM_032444.2 protein sequence:; P1122L; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.