cDNA Clone KLC1

• Plasmid: pCMV5-HA KLC1J
• Parent Plasmid: pCMV5 HA1
• Expressed: HA KLC1J
• Genbank: DAA01298.1 GI:33186850*
• Tag: HA
• Species: Human

Sequence of Insert

ggatccatgtccacaatggtgtacataaaggaagacaagttggagaagct
tacacaggatgaaattatttctaagacaaagcaagtaattcaggggctgg
aagctttgaagaatgagcacaattccattttacaaagtttgctggagaca
ctgaagtgtttgaagaaagatgatgaaagtaatttggtggaggagaaatc
aaacatgatccggaagtcactggagatgttggagctcggcctgagtgagg
cacaggttatgatggctttgtcaaatcacctgaatgctgtggagtccgag
aagcagaaactgcgtgcgcaggttcgtcgtctgtgccaggagaatcagtg
gctacgggatgaactggccaacacgcagcagaaactgcagaagagtgagc
agtctgtggctcaactggaggaggagaagaagcatctggagtttatgaat
cagctaaaaaaatatgatgacgacatttccccatccgaggacaaagacac
tgattctaccaaagagcctctggatgaccttttccccaatgatgaagacg
acccagggcaaggaatccagcagcagcacagcagtgcagccgcggctgcc
cagcagggcggctacgagatccccgcgcggctgcggacgctccacaacct
ggtgatccagtacgcctcgcaggggcgctacgaggtagctgtgcccctct
gcaagcaggccctggaggacctggagaagacttcaggacacgaccacccg
gacgtggccaccatgctcaacatcctggccttggtgtacagggatcagaa
taaatacaaagatgcagctaacctactgaatgatgccttggctattcgtg
agaaaactttgggcaaagatcatcctgcggtggcggcgactttgaataac
cttgcagtcctttatggtaaaagagggaagtacaaagaagcagagccgtt
gtgtaaaagagctctggaaatccgagaaaaggttttggggaaggatcacc
ccgatgttgccaagcagttaaataacttggccttactgtgccagaaccag
ggcaagtatgaagaagtagaatattattatcaaagagccctcgagatcta
ccagacaaaactgggacctgatgaccccaacgtggctaagacgaaaaata
acctggcatcctgctatttgaaacaaggaaagttcaagcaagcagaaaca
ctgtacaaagagattctcactcgtgcacatgaaagggagtttggttctgt
agatgatgaaaataaacccatctggatgcatgctgaagaaagagaagaat
gcaaaggaaagcaaaaggatgggacatcttttggagagtatggcggctgg
tacaaagcctgcaaagttgatagtccaactgttacaaccactctaaaaaa
ccttggggcactttacagacgtcaaggcaaatttgaagctgcagaaacgt
tagaagaagctgctatgaggtctcgtaaacagggtcttgacaatgttcac
aaacagagggtggcagaagtgctcaatgaccctgagaacatggagaagcg
caggagccgtgagagcctcaacgtggacgtggtcaagtacgagagtggcc
ctgacggaggggaggaagtgagtatgagcgtagagtggaacggggatggc
actggatctttaaaacgcagtggttcctttagcaaactccgggcttccat
tagacgcagcagtgagaagctggttaggaagctgaagggaggaagttcac
gagagagtgagccaaagaaccccggcatgaagcgtgccagctctctgaat
gtccttaacgtgggtggcaaggctgctgaagatcgctttcaagaacgaaa
taattgtctggccgactcgcgagctctgagtgccagccacactgacctgg
cccactgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEH
NSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMMAL
SNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLE
EEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQ
QQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALED
LEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKD
HPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQL
NNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYL
KQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKD
GTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMR
SRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEV
SMSVEWNGDGTGSLKRSGSFSKLRASIRRSSEKLVRKLKGGSSRESEPKN
PGMKRASSLNVLNVGGKAAEDRFQERNNCLADSRALSASHTDLAH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16340
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 72828 Daltons