cDNA Clone TIE2

• Plasmid: pFastBac.HTb TIE2 771-end
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TIE2 771-end
• Genbank: NM_000459.3 GI:183396798
• Tag: 6His

Sequence of Insert

ggatccCAATTGAAGAGAGCAAATGTGCAAAGGAGAATGGCCCAAGCCTT
CCAAAACGTGAGGGAAGAACCAGCTGTGCAGTTCAACTCAGGGACTCTGG
CCCTAAACAGGAAGGTCAAAAACAACCCAGATCCTACAATTTATCCAGTG
CTTGACTGGAATGACATCAAATTTCAAGATGTGATTGGGGAGGGCAATTT
TGGCCAAGTTCTTAAGGCGCGCATCAAGAAGGATGGGTTACGGATGGATG
CTGCCATCAAAAGAATGAAAGAATATGCCTCCAAAGATGATCACAGGGAC
TTTGCAGGAGAACTGGAAGTTCTTTGTAAACTTGGACACCATCCAAACAT
CATCAATCTCTTAGGAGCATGTGAACATCGAGGCTACTTGTACCTGGCCA
TTGAGTACGCGCCCCATGGAAACCTTCTGGACTTCCTTCGCAAGAGCCGT
GTGCTGGAGACGGACCCAGCATTTGCCATTGCCAATAGCACCGCGTCCAC
ACTGTCCTCCCATCATCTCCTTCACTTCGCTGCCGACGTGGCCCGGGGCA
TGGACTACTTGAGCCAAAAACAGTTTATCCACAGGGATCTGGCTGCCAGA
AACATTTTAGTTGGTGAAAACTATGTGGCAAAAATAGCAGATTTTGGATT
GTCCCGAGGTCAAGAGGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAG
TGCGCTGGATGGCCATCGAGTCACTGAATTACAGTGTGTACACAACCAAC
AGTGATGTATGGTCCTATGGTGTGTTACTATGGGAGATTGTTAGCTTAGG
AGGCACACCCTACTGCGGGATGACTTGTGCAGAACTCTACGAGAAGCTGC
CCCAGGGCTACAGACTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTAT
GATCTAATGAGACAATGCTGGCGGGAGAAGCCTTATGAGAGGCCATCATT
TGCCCAGATATTGGTGTCCTTAAACAGAATGTTAGAGGAGCGAAAGACCT
ACGTGAATACCACGCTTTATGAGAAGTTTACTTATGCAGGAATTGACTGT
TCTGCTGAAGAAGCGGCCTAGcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSQLKRANVQRRMAQAFQNVREEP
AVQFNSGTLALNRKVKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKAR
IKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGAC
EHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSHHLL
HFAADVARGMDYLSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQEVY
VKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGM
TCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSL
NRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU16327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 43848 Daltons