cDNA Clone Cullin7

• Synonyms: CUL7
• Plasmid: pFBDual GST-Cullin7 6His-Rbx1
• Parent Plasmid: pFastbacDual
• Expressed: GST-Cullin7
• Genbank: NM_014780
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGTGGGAGAACTCCGCTACAGGGAATTCAGGGTGCCCCTGGGGCCCGGCTTACATGCCTATCCTGATGAGCTGATCCGCCAGCGCGTGGGCCATGATGGGCATCCTGAGTACCAGATCCGTTGGCTCATCCTGCGGCGTGGCGATGAGGGGGACGGGGGCTCTGGCCAAGTGGACTGCAAGGCTGAGCACATCCTGCTGTGGATGTCCAAGGATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGGATGGCCAGGTCATCGGGCCCTCCCAGGAGTCTGCAGGGGAGGTTGGGGCCCTGGACAAATCTGTGCTGGAGGAGATGGAAACCGACGTGAAGTCCCTCATTCAGAGAGCCCTTCGGCAGCTGGAGGAGTGTGTGGGCACTATCCCTCCTGCTCCTCTACTTCACACTGTCCACGTGCTCAGCGCCTATGCCAGCATTGAGCCCCTCACTGGAGTATTCAAGGACCCAAGGGTCCTGGACTTGCTCATGCACATGTTGAGTAGTCCTGATTATCAGATTCGCTGGAGTGCAGGCCGGATGATACAAGCCCTGTCCTCCCATGACGCTGGGACCCGGACTCAGATCCTTCTGTCACTGAGCCAACAAGAAGCCATTGAGAAACACCTGGATTTTGACAGCCGCTGTGCTCTGCTAGCACTGTTTGCACAGGCCACGCTCTCTGAACACCCCATGTCTTTCGAGGGCATTCAGCTACCACAGGTCCCAGGAAGGGTGCTCTTCTCCCTGGTGAAGCGGTATTTGCATGTCACCTCGCTCCTGGATCAGCTGAACGACAGTGCTGCGGAGCCAGGAGCCCAGAACACCTCTGCTCCTGAGGAGTTGAGTGGGGAGAGGGGTCAACTGGAGCTGGAGTTCAGTATGGCCATGGGCACCCTGATCTCGGAGCTGGTGCAAGCCATGCGCTGGGACCAGGCCTCAGACAGACCAAGGAGCTCAGCACGGTCCCCCGGTTCCATCTTCCAGCCTCAGCTGGCAGATGTGAGCCCAGGGCTCCCCGCTGCCCAGGCTCAGCCCTCCTTCAGGAGGTCAAGACGTTTTCGCCCTCGTTCTGAGTTCGCAAGTGGCAATACCTATGCTTTGTATGTGCGGGACACACTGCAGCCGGGGATGCGAGTGCGGATGCTGGATGATTATGAGGAGATCAGTGCCGGGGATGAGGGCGAGTTTCGGCAGAGCAACAACGGTGTGCCTCCTGTGCAGGTATTTTGGGAGTCAACAGGCCGCACCTATTGGGTGCACTGGCACATGCTGGAGATCTTGGGCTTTGAGGAAGACATTGAGGACATGGTTGAGGCTGATGAGTACCAAGGGGCAGTGGCCAGTAGAGTCCTGGGTAGAGCCCTGCCTGCCTGGCGCTGGAGGCCCATGACAGAACTCTATGCTGTGCCTTATGTGCTGCCTGAGGATGAGGACACTGAGGAGTGTGAACACCTGACCCTGGCTGAGTGGTGGGAACTCCTCTTCTTCATCAAGAAGCTGGATGGACCTGACCATCAGGAGGTTCTCCAGATCCTCCAGGAGAACCTAGATGGGGAGATTCTGGATGATGAGATCCTAGCTGAACTGGCCGTGCCCATAGAATTGGCCCAGGACTTGCTGCTGACTCTGCCACAGCGACTCAATGACAGTGCCCTCAGGGACCTGATCAACTGCCATGTCTACAAGAAGTATGGGCCTGAAGCCCTAGCAGGGAACCAAGCCTACCCATCCCTTCTAGAAGCCCAAGAAGATGTCCTCCTGCTAGACGCGCAGGCCCAGGCTAAGGACTCAGAAGATGCAGCCAAAGTGGAAGCAAAAGAACCCCCATCTCAGAGTCCCAACACTCCCCTGCAGCGTCTGGTGGAGGGTTATGGTCCAGCTGGGAAAATCCTCCTGGATCTAGAGCAAGCCCTCAGCTCAGAGGGGACCCAGGAGAACAAGGTCAAGCCACTCCTGCTGCAGCTGCAGCGGCAGCCGCAGCCCTTCCTGGCACTGATGCAGAGCCTGGACACTCCGGAGACTAACAGGACCCTGCACCTGACTGTGCTGAGAATCCTGAAGCAGCTGGTGGACTTCCCCGAGGCACTGCTGCTCCCCTGGCACGAGGCCGTGGATGCCTGCATGGCCTGCCTGCGGTCCCCAAACACTGATCGAGAGGTGCTCCAGGAACTGATTTTCTTCCTGCACCGCCTGACCTCAGTGAGCAGGGACTATGCCGTGGTGCTGAATCAGCTGGGAGCAAGAGACGCTATCTCCAAGGCCCTGGAAAAGCACCTGGGAAAGCTGGAGCTGGCTCAGGAGCTGCGGGACATGGTGTTCAAGTGTGAGAAGCATGCCCACCTCTACCGCAAACTCATCACCAACATCCTGGGAGGCTGCATCCAGATGGTGCTGGGCCAGATCGAAGACCACAGACGAACCCACCGGCCCATCAACATCCCTTTCTTTGATGTGTTCCTCAGATACCTGTGCCAGGGCTCCAGTGTGGAAGTGAAGGAGGACAAGTGCTGGGAGAAGGTGGAGGTGTCCTCCAACCCGCACCGGGCCAGCAAGCTGACGGACCACAACCCCAAGACCTATTGGGAGTCCAACGGCAGCGCCGGCTCCCACTACATCACCCTGCACATGCGCCGGGGCATCCTCATCAGGCAACTGACTCTGCTTGTGGCTAGTGAGGACTCGAGTTACATGCCGGCCCGAGTGGTGGTGTGCGGGGGTGATAGCACTAGCTCTCTTCACACGGAACTCAACTCGGTGAATGTGATGCCCTCTGCCAGCCGGGTGATCCTCCTGGAGAACCTGACCCGCTTCTGGCCCATCATCCAGATCCGCATAAAGCGCTGCCAGCAGGGTGGCATTGATACGCGCATTCGGGGGTTAGAGATCCTAGGCCCCAAGCCCACGTTCTGGCCAGTGTTCCGGGAGCAGCTCTGTCGTCACACACGCCTCTTCTACATGGTTCGGGCACAGGCCTGGAGCCAGGACATGGCAGAGGACCGCAGGAGCCTCCTGCACCTGAGTTCTAGACTCAACGGTGCTCTGCGCCAGGAGCAGAATTTTGCTGACCGCTTCCTCCCTGATGACGAGGCTGCCCAAGCTCTGGGCAAGACCTGCTGGGAGGCCCTGGTCAGCCCCGTGGTGCAGAACATCACCTCCCCTGATGAGGATGGCATTAGCCCCCTGGGTTGGCTGCTGGACCAGTACCTGGAGTGTCAGGAAGCTGTCTTCAACCCCCAGAGCCGCGGCCCAGCTTTCTTCTCGCGGGTGCGCCGTCTCACTCACCTGCTGGTGCATGTCGAGCCCTGTGAGGCACCCCCTCCTGTGGTGGCCACTCCTCGGCCCAAAGGCAGAAACAGAAGCCACGACTGGAGCTCCTTGGCTACCCGGGGCCTTCCAAGCAGCATCATGAGAAACCTGACGCGCTGTTGGCGGGCCGTGGTGGAGAAGCAGGTGAACAATTTTCTGACCTCATCCTGGCGGGATGATGACTTTGTGCCACGCTACTGTGAGCACTTTAATATTCTGCAGAACTCAAGCTCTGAACTGTTTGGGCCTCGGGCAGCCTTCTTGCTGGCGCTGCAAAATGGCTGTGCGGGAGCCTTGCTGAAGCTCCCTTTTCTCAAAGCTGCCCACGTGAGTGAGCAGTTCGCCCGGCACATTGACCAGCAGATCCAGGGCAGCCGGATCGGTGGAGCCCAGGAAATGGAGAGGCTGGCACAGCTGCAGCAATGCCTGCAAGCTGTCCTGATTTTCTCCGGCTTGGAGATAGCCACCACTTTTGAGCATTATTACCAGCACTACATGGCGGACCGTCTCCTGGGCGTGGTCTCGAGCTGGCTGGAGGGGGCCGTGCTGGAGCAGATCGGTCCCTGCTTCCCCAACCGCCTCCCCCAGCAGATGTTGCAGAGCCTGAGCACCTCTAAGGAGCTGCAGCGCCAGTTCCACGTCTACCAGCTCCAGCAGCTGGATCAGGAACTCCTGAAGCTGGAGGATACAGAGAAGAAAATACAGGTGGGCCTTGGGGCCAGTGGCAAGGAGCACAAGAGCGAGAAGGAAGAGGAAGCTGGGGCAGCAGCAGTGGTGGATGTGGCGGAGGGAGAGGAGGAAGAGGAGGAGAATGAGGACCTCTACTATGAAGGGGCAATGCCAGAAGTGTCTGTGCTTGTCCTGTCCCGACACTCCTGGCCTGTTGCCTCAATCTGCCACACACTGAACCCCAGAACCTGCCTGCCCTCCTACCTGAGGGGCACTTTGAACAGATACTCCAACTTCTACAACAAGAGTCAGAGCCACCCTGCCCTTGAGCGAGGCTCACAGAGGCGACTGCAGTGGACGTGGCTGGGCTGGGCTGAGCTGCAGTTTGGGAACCAGACCCTGCATGTGTCCACCGTGCAGATGTGGCTACTGCTGTATCTCAACGACCTGAAGGCGGTCTCTGTGGAGAGTCTGCTGGCGTTCTCAGGGCTCTCCGCAGACATGCTCAATCAGGCGATTGGGCCCCTCACCTCTTCAAGAGGCCCCCTGGACCTTCACGAGCAAAAGGATATACCAGGAGGGGTCCTCAAGATTCGAGATGGCAGCAAGGAACCCAGGTCGAGATGGGACATTGTGCGGCTCATCCCACCTCAGACGTACCTGCAAGCTGAGGGTGAAGACGGCCAGAACTTGGAGAAGAGACGGAATCTTCTGAACTGCCTCATCGTCCGAATCCTCAAGGCCCATGGAGATGAGGGGCTGCACATTGACCAGCTTGTCTGTCTGGTGCTGGAGGCTTGGCAGAAGGGCCCGTGTCCTCCCAGGGGTTTGGTCAGCAGCCTTGGTAAGGGGTCTGCATGCAGCAGCACTGACGTCCTCTCCTGCATCCTACACCTCCTGGGCAAGGGCACGCTGAGACGCCATGACGACCGGCCCCAGGTGCTGTCCTATGCAGTCCCTGTGACTGTCATGGAGCCTCACACTGAGTCCCTGAACCCAGGCTCCTCAGGCCCCAACCCACCCCTCACCTTCCATACCCTACAGATTCGCTCCCGGGGTGTGCCCTATGCCTCCTGCACTGCCACCCAGAGCTTCTCTACCTTCCGGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVGELRYREFRVPLGPGLHAYPDELIRQRVGHDGHPEYQIRWLILRRGDEGDGGSGQVDCKAEHILLWMSKDEIYANCHKMLGEDGQVIGPSQESAGEVGALDKSVLEEMETDVKSLIQRALRQLEECVGTIPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLDLLMHMLSSPDYQIRWSAGRMIQALSSHDAGTRTQILLSLSQQEAIEKHLDFDSRCALLALFAQATLSEHPMSFEGIQLPQVPGRVLFSLVKRYLHVTSLLDQLNDSAAEPGAQNTSAPEELSGERGQLELEFSMAMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLADVSPGLPAAQAQPSFRRSRRFRPRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGDEGEFRQSNNGVPPVQVFWESTGRTYWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLGRALPAWRWRPMTELYAVPYVLPEDEDTEECEHLTLAEWWELLFFIKKLDGPDHQEVLQILQENLDGEILDDEILAELAVPIELAQDLLLTLPQRLNDSALRDLINCHVYKKYGPEALAGNQAYPSLLEAQEDVLLLDAQAQAKDSEDAAKVEAKEPPSQSPNTPLQRLVEGYGPAGKILLDLEQALSSEGTQENKVKPLLLQLQRQPQPFLALMQSLDTPETNRTLHLTVLRILKQLVDFPEALLLPWHEAVDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDAISKALEKHLGKLELAQELRDMVFKCEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHRPINIPFFDVFLRYLCQGSSVEVKEDKCWEKVEVSSNPHRASKLTDHNPKTYWESNGSAGSHYITLHMRRGILIRQLTLLVASEDSSYMPARVVVCGGDSTSSLHTELNSVNVMPSASRVILLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEILGPKPTFWPVFREQLCRHTRLFYMVRAQAWSQDMAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSPVVQNITSPDEDGISPLGWLLDQYLECQEAVFNPQSRGPAFFSRVRRLTHLLVHVEPCEAPPPVVATPRPKGRNRSHDWSSLATRGLPSSIMRNLTRCWRAVVEKQVNNFLTSSWRDDDFVPRYCEHFNILQNSSSELFGPRAAFLLALQNGCAGALLKLPFLKAAHVSEQFARHIDQQIQGSRIGGAQEMERLAQLQQCLQAVLIFSGLEIATTFEHYYQHYMADRLLGVVSSWLEGAVLEQIGPCFPNRLPQQMLQSLSTSKELQRQFHVYQLQQLDQELLKLEDTEKKIQVGLGASGKEHKSEKEEEAGAAAVVDVAEGEEEEEENEDLYYEGAMPEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSHPALERGSQRRLQWTWLGWAELQFGNQTLHVSTVQMWLLLYLNDLKAVSVESLLAFSGLSADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRSRWDIVRLIPPQTYLQAEGEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGKGSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQIRSRGVPYASCTATQSFSTFR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU23051
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: nt 2438-2439 (gg) divergent from reference sequence (aa). Confers Q813R substitution. Not a PCR error, found in other clones from various libraries