cDNA Clone Lag2

• Plasmid: pRS413 Lag2 promoter (0.5kb) GFP-Lag2
• Parent Plasmid: pRS413
• Expressed: GFP-Lag2
• Tag: GFP
• Species: Saccharomyces cerevisiae

Sequence of Insert

gggcccGAATATACCACTTCGTTATAAGTGATTACATCTCGTAACGCATTCCTCTTGTTCAGTGGTAGGGGAGGCCGATGGTAGTGGAATAATACGCTTTTATGGGCCATTGGTATTACTATTTATCTTCGTTATAATAAATAGTACACGTTGCAGACATATTTGGAGGAGAGTTTTTAAAGTACTGTTGGGGTGCTCTTGCTGTGCATTGTAAGTAATCGTGTCCCAGTGGCCGAGTGGTTAAGGCGATGCCCTGCTATTTCCTCAGAAAAGCAATTAGGCATTGGGTTTTACCTGCGCAGGTTCGAATCCTGTCTGTGACGCATTTCTTTTTTGTGAATTCTTGCTTCTTATCCTTCTACAATCCTTTCGTGACATTTATTGAATCATCTCTACAAAATTTTTACACATAGCTAAGGTTAGCTTGTTCTACATTACACAAGTGGTAGCAAAATCGTTTACATTGCACTCAGCTATATCAACATCACGTTCTACCGCAACTAGTAGtcgac

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGGGGGGGGTMSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNELLLPVLVEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMIHANRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMCDVDAAQLSHSLSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILNDVFTIAQDYSATNTNESIDRITEKLLLTSTQLTHPVDLENLCPKMKYNTLAAVSRIWYKFGPIVDKLFTNRLLPVLFPPQMGEECNVEDVLEIVHNFHPYFSIRRLKDNRPLLSDSTISQLREGLFGMLSILNDSLTRTQNENDHGSDNLIDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDADDINVEKNDEATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILSKETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSELSVLQLSIEVIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKAGNFTQKIDEGVTLRTMILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLGVRDLSFQLLDQILRTYYNYLIGIDWEWYKDDFYQVLQETCIKKDINTNLLLQFPPYLPHD*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU23044
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 23029-derived promoter sequence replaces 21586 promoter sequence (Apa1-Sal1) to enable direct comparison of 0.5kb Lag2 Pr with the 1kb upstream from ORF in SC21586.