cDNA Clone Lag2

• Plasmid: pRS413 Lag2 promoter (1.5kb) GFP-Lag2
• Parent Plasmid: pRS413
• Expressed: GFP-Lag2
• Tag: GFP
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGGCCCGTGCTTCACTTTGACCATAAGCGTCTCCTTGGGCTTGGCAGGAGCATCGGTGGATGTTGGAGCTACGGGCTTCGTAACACTTTTCGACTTATCAGTAGAATAAAATCTCAAAGATAAAGGATAGTTGGGCTGTACTCCTTGCGCTCTCAAAGGCCAATGACACGAGGGAATAGTGGAAGCTATTAATCTAGGGCCTTGATTTTTAACAAAATACAAGCTTGCTTGACGTCTTGTCACACATGACGCTCTTGATGCGAAATTCAACATCTTAGTATATTATCTGTAGTGATAGAGATAGCGTATATTATGCGCTGCCTGAAATGCACTGCGCTGTTCATATTCATCACCGTGGCAATGACTGATCCATTTATTTGGAGATGATTCAACGAATAATTTTTCACCGCGGGATTATCGCCCAAGACGGGGTCGTTTGAGGGTAATACCGTGGCTAGCTTTATGGCTTGTGAAACTATTGCCAGTTATGTGGTTACGTATAATCTACCGTATGTGTGTGTGTATTTATTTATGTAGGTTGCTAATGCTTTGGTGATCGTTTAAAGGAACTTGGACGCAACCCTCCCACGGGTGCCAGGGCTTGAAATCTCTTTCAATGCTGCTATTTTGCGCGACTCTGGGTACACCTTATACCCCTTGTTTCTATGGTTAAACCAATTGAATCTCCAGCAGAGCTCGTGAGCAAATAGCTCGCCGAAGCCGAGCATCATGCCGTTGAGGAAGGGCAAAAGTAGGTTGATGGAGCACGAGCCTACAAAGGATACAAGGCTCTGCACAAGTGGGCTCTCGTCGGCACTCACTGTGTTCTGCACCTCCATACAGTCTTTGTCTTCTGATTCGTCATCTGACACGCTCTCCCAGAATCCCACAACCTCTGTCATATTTCTGTCTTGTGGCAGTTTCGTAAGAAGGGGGGTGATGTTTCTAATATTACAGTGATGTTCTATTGTTCAAGTAAAGTGTATGGCTGTTTCTTATGGCGCGGAATATACCACTTCGTTATAAGTGATTACATCTCGTAACGCATTCCTCTTGTTCAGTGGTAGGGGAGGCCGATGGTAGTGGAATAATACGCTTTTATGGGCCATTGGTATTACTATTTATCTTCGTTATAATAAATAGTACACGTTGCAGACATATTTGGAGGAGAGTTTTTAAAGTACTGTTGGGGTGCTCTTGCTGTGCATTGTAAGTAATCGTGTCCCAGTGGCCGAGTGGTTAAGGCGATGCCCTGCTATTTCCTCAGAAAAGCAATTAGGCATTGGGTTTTACCTGCGCAGGTTCGAATCCTGTCTGTGACGCATTTCTTTTTTGTGAATTCTTGCTTCTTATCCTTCTACAATCCTTTCGTGACATTTATTGAATCATCTCTACAAAATTTTTACACATAGCTAAGGTTAGCTTGTTCTACATTACACAAGTGGTAGCAAAATCGTTTACATTGCACTCAGCTATATCAACATCACGTTCTACCGCAACTAGTAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGGGGGGGGTMSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNELLLPVLVEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMIHANRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMCDVDAAQLSHSLSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILNDVFTIAQDYSATNTNESIDRITEKLLLTSTQLTHPVDLENLCPKMKYNTLAAVSRIWYKFGPIVDKLFTNRLLPVLFPPQMGEECNVEDVLEIVHNFHPYFSIRRLKDNRPLLSDSTISQLREGLFGMLSILNDSLTRTQNENDHGSDNLIDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDADDINVEKNDEATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILSKETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSELSVLQLSIEVIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKAGNFTQKIDEGVTLRTMILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLGVRDLSFQLLDQILRTYYNYLIGIDWEWYKDDFYQVLQETCIKKDINTNLLLQFPPYLPHD*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU21921
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 21902 derived promoter (1.5kb upstream from ORF) replaces that in 21586 (1kb upstream from ORF) to enable direct comparison upstream sequences.