cDNA Clone USP11

• Synonyms: UHX1
• Plasmid: pFB DUAL 6P-1 USP11
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-USP11
• Genbank: NM_004651.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGTTTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCTCGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTCGCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGTGACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCCAGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGGGCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAGGCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCATCAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAGGGACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCATTACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAACGCAAGGTCATAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTAGAACTGCTGCTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCAGTTCAGCCATACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGCGGTTTCTGGTGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCAGAAGGCTCTTTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGCGGCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATGGCACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAGAGGATGAGGACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCTGGGCAACACGTGCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGCCACAGCTCACCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAACTTCCGCAACCCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGACCTGGTGAAGCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATGTGTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAGCAGCATGACTCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAGGACCTTAATCGGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTGCTGGGCGACCGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAACGGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTCCACGCTGGTGTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCTTCTGCTACCTCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAGGTCTTCTTTATCCCCATGGACCCGCGCCGCAAGCCAGAGCAGCACCGGCTCGTGGTCCCCAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCAAACACACGGGCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGTCACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTTGGACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCATTGAGGGCTCAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCACCCCTGCCCGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGGACACCCCCTCCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCCTGTATAACGTCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAACTCAGATGATGAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAAAGATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGCCAGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCCTGGACAATTGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAGCAGCTGTTCACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCCCTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGGGAGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTACGTGAAGCATGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGCTGCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAAAACCCCTGGTACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAGCTGGACCTGTGGATGCTGCC-GGAGATTCTCATCATCCACCTGAAACGCTTTTCCTACACCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTTCCTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGAGTCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATTATGGGGGCATGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGACAGCGGCCAGTGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGAGAATCAGATCGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGGACGTGGCGCGACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCCTCCCCTGCCTGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAVAPRLFGGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAAAAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWEAYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAAAWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKSHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMDAN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU37747
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains T>C silent mutation to KO internal BamHI site