cDNA Clone USP4

• Synonyms: Ubiquitin specific peptidase 4 (proto-oncogene), (USP4), transcript variant 1
• Plasmid: pET156P-1-USP4
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP4
• Genbank: NM_003363
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGACTCAGAAGTCCGAGCTTGGACCCTTAATGAGGACCACACTCCAACGCGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAAGTATGTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATTTCCTGGCCCAATAGACAACTCTGGGCTATTTTCAGATCCTGAGAGTCAGACCTTGAAAGAACACTTAATTGATGAATTGGACTATGTATTGGTCCCTACCGAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCAACCCATCGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTCGAGGTGTATTTGCTGGAACTGAAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAACCATCGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACACGGCTCTGGAACAAATACATGAGCAACACCTACGAGCAGTTGAGCAAGCTAGACAACACTGTCCAGGATGCTGGGCTATACCAGGGTCAGGTGCTAGTAATTGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAAAATCAAGCACTGCGCCTAGCAGAAATTTTACTACCTCTCCAAAATCATCAGCAAGTCCCTATTCCTCAGTGTCTGCCTCTCTCATTGCAAATGGTGATAGCACTAGCACCTGTGGGATGCACAGTTCCGGTGTCAGCAGGGGTGGATCTGGCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAACCTGGGCTCTGTGGACTTGGAAACCTGGGAAACACCTGCTTCATGAACTCCGCTTTGCAGTGTTTGAGCAACACTGCACCACTGACTGACTACTTTCTCAAAGATGAGTATGAAGCCGAAATCAACAGAGACAACCCTCTGGGGATGAAAGGGGAAATTGCAGAAGCCTATGCTGAACTCATTAAGCAGATGTGGTCTGGAAGGGACGCCCATGTGGCACCTCGCATGTTCAAAACTCAAGTAGGACGTTTTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCTGCTGGCCTTTCTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAAGCCCTACTTGGAGCTGAAGGATGCCAATGGGCGGCCAGATGCGGTGGTGGCAAAGGAAGCCTGGGAGAATCACAGGTTGAGGAATGATTCTGTGATTGTGGATACTTTCCATGGCCTCTTCAAATCTACTTTGGTTTGCCCAGAATGTGCTAAGGTTTCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCACTGCCCTTGAAGAAAGATCGAGTTATGGAGGTTTTCCTGGTTCCTGCTGACCCTCACTGCAGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGGCTGTGTCCGACCTGTGCGAGGCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCGCAGATGTGTATAATCACCGATTCCACAAAATTTTCCAAATGGATGAAGGTTTAAACCACATCATGCCTCGGGATGACATTTTCGTGTACGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTTCCAGTCTACTTCAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCAGCGCTATATGGGCAGCCACTATTGCTTTCTGTCCCCAAGCACAAGTTAACCCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTATGTGAAACAGCCTTTACCTGATGAGTTTGGCAGCTCACCCTTGGAGCCAGGGGCCTGCAATGGCTCCAGGAACAGCTGTGAAGGAGAAGATGAGGAAGAAATGGAGCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAACAGAAGGCAGTGGGGAAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAAGGCCAGCCCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTATGGAACAGCTGACATAAATTCACTTGCAGCTGATGGAAAACTACTTAAACTCAACTCTCGATCTACACTGGCCATGGATTGGGACAGTGAAACTCGGAGACTTTACTATGATGAGCAAGAATCTGAGGCCTACGAGAAGCATGTGAGCATGTTGCAGCCTCAGAAGAAGAAGAAGACCACAGTGGCCCTGAGAGACTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGACCCCTGGTACTGTCCCAACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATGGTCCTTGCCCAAGATCCTGGTGGTCCACCTCAAACGTTTCTCCTACAACAGATACTGGAGGGATAAGCTCGACACAGTCGTAGAATTCCCAATCAGAGGGCTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCAAGGCCTTATGTGTACGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGCCACTACACTGCATATGCGAAGAACAAACTGAATGGTAAATGGTATTACTTTGATGATAGCAACGTGTCCCTGGCCTCTGAGGATCAGATAGTGACTAAAGCAGCTTATGTGCTATTTTACCAACGTCGAGATGATGAATTTTATAAGACACCTTCACTTAGCAGTTCTGGTTCCTCTGATGGAGGGACACGACCAAGCAGCTCTCAGCAGGGCTTTGGGGATGATGAGGCTTGCAGCATGGACACCAACTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN*

• Vector Type: Bacterial
• DU Number: DU23032
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee