cDNA Clone RNF220

• Plasmid: pEFIRES-P-eYFP C1-RNF220 g420a, t426c, c432t
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-RNF220 g420a, t426c, c432t
• Genbank: NM_018150.2
• Tag: YFP
• Species: Human

Sequence of Insert

GGATCCATGGACTTACACCGGGCAGCCTTCAAGATGGAGAACTCATCCTA
CCTTCCCAACCCTCTGGCATCCCCAGCACTGATGGTCCTGGCATCCACGG
CTGAGGCCAGCCGTGATGCTTCCATCCCTTGTCAGCAGCCACGACCCTTT
GGTGTACCTGTCTCAGTTGACAAGGACGTGCATATTCCTTTCACCAACGG
TTCCTATACCTTTGCCTCTATGTACCATCGGCAAGGTGGGGTGCCAGGCA
CTTTTGCCAATCGTGATTTCCCCCCTTCTCTACTACACCTCCACCCTCAA
TTTGCTCCCCCAAATCTAGATTGCACCCCAATCAGTATGCTGAATCATAG
TGGTGTGGGGGCTTTCCGGCCCTTTGCCTCCACCGAGGACCGGGAGAGCT
ATCAGTCAGCCTTTACGCCGGCCAAACGACTCAAGAATTGCCATGACACA
GAGTCTCCCCACTTGCGCTTCTCAGATGCAGATGGCAAGGAATATGACTT
TGGGACACAGCTGCCATCTAGCTCCCCCGGTTCACTAAAGGTTGATGACA
CTGGGAAGAAGATTTTTGCTGTCTCTGGCCTCATTTCTGATCGGGAAGCC
TCATCTAGCCCAGAGGATCGGAATGACAGATGTAAGAAGAAAGCAGCGGC
ATTGTTCGACAGCCAGGCCCCAATTTGCCCCATCTGCCAGGTCCTGCTGA
GGCCCAGTGAGCTGCAGGAGCATATGGAGCAGGAACTGGAGCAGCTAGCC
CAACTGCCCTCGAGCAAGAATTCCCTTCTGAAGGATGCCATGGCTCCAGG
CACCCCAAAGTCCCTCCTGTTGTCTGCTTCCATCAAGAGGGAAGGAGAGT
CTCCAACGGCATCACCCCACTCATCTGCCACCGATGACCTCCACCATTCA
GACAGATACCAGACCTTTCTGCGAGTACGAGCCAACCGGCAGACCCGACT
GAATGCTCGGATTGGGAAAATGAAACGGAGGAAGCAAGATGAAGGGCAGA
GGGAAGGCTCCTGCATGGCTGAGGATGATGCTGTGGACATCGAGCATGAG
AACAACAACCGCTTTGAGGAGTATGAGTGGTGTGGACAGAAGCGGATACG
GGCCACCACTCTCCTGGAAGGTGGCTTCCGAGGCTCTGGCTTCATCATGT
GCAGCGGCAAAGAGAACCCGGACAGTGATGCTGACTTGGATGTGGATGGG
GATGACACTCTGGAGTATGGGAAGCCACAATACACAGAGGCTGATGTCAT
CCCCTGCACAGGCGAGGAGCCTGGTGAAGCCAAGGAGAGAGAGGCACTTC
GGGGCGCAGTCCTAAATGGCGGCCCTCCCAGCACGCGCATCACACCTGAG
TTCTCTAAATGGGCCAGTGATGAGATGCCATCCACCAGCAATGGTGAAAG
CAGCAAGCAGGAGGCCATGCAGAAGACCTGCAAGAACAGCGACATCGAGA
AAATCACCGAAGATTCAGCTGTGACCACGTTTGAGGCTCTGAAGGCTCGG
GTCAGAGAACTTGAACGGCAGCTATCTCGTGGGGACCGTTACAAATGCCT
CATCTGCATGGACTCGTACTCGATGCCCCTAACGTCCATCCAGTGTTGGC
ACGTGCACTGCGAGGAGTGCTGGCTGCGGACCCTGGGTGCCAAGAAGCTC
TGCCCTCAGTGCAACACGATCACAGCGCCCGGAGACCTGCGGAGGATCTA
CTTGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMDLHRA
AFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDK
DVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDC
TPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFS
DADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRN
DRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPSSKNS
LLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLR
VRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENNNRFEEY
EWCGQKRIRATTLLEGGFRGSGFIMCSGKENPDSDADLDVDGDDTLEYGK
PQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDE
MPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQL
SRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTIT
APGDLRRIYL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20625
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP-RNF220 g420a, t426c, c432t. EF1a promoter, IRES and puromycin selection cassette; Parental PLasmid 20522; Estimated MW of expressed protein : 90240 Daltons