cDNA Clone ACTN4

• Plasmid: pCMV5-HA ACTN4 (actinin, alpha 4)
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-ACTN4
• Genbank: NM_004924
• Tag: HA
• Species: Human

Sequence of Insert

gaattcatggtggactaccacgcggcgaaccagtcgtaccagtacggccccagcagcgcgggcaatggcgctggcggcgggggcagcatgggcgactacatggcccaggaggacgactgggaccgggacctgctgctggacccggcctgggagaagcagcagcgcaagaccttcacggcatggtgcaactcccacctgcggaaggcaggcacacagatcgagaacattgatgaggacttccgagacgggctcaagctcatgctgctcctggaggtcatatcaggggagcggttacctaagccggag
cgggggaagatgagagtgcacaaaatcaacaatgtgaacaaagcgctgga
ctttattgccagcaaaggcgtcaagctggtctccatcggggcagaagaga
ttgtggacggcaacgcaaagatgaccctgggaatgatctggaccatcatc
cttaggttcgccatccaggacatctccgtggaagagacctcggccaagga
agggctccttctctggtgccagagaaagacagccccgtataagaacgtca
atgtgcagaacttccacatcagctggaaggatggtcttgccttcaatgcc
ctgatccaccggcacagaccagagctgattgagtatgacaagctgaggaa
ggacgaccctgtcaccaacctgaacaatgccttcgaagtggctgagaaat
acctcgacatccccaagatgctggatgcagaggacatcgtgaacacggcc
cggcccgacgagaaggccataatgacctatgtgtccagcttctaccatgc
cttttcaggagcgcagaaggctgaaactgccgccaaccggatctgtaagg
tgctggctgtcaaccaagagaacgagcacctgatggaggactacgagaag
ctggccagcgacctcctggagtggatccggcgcaccatcccctggctgga
ggaccgtgtgccccaaaagactatccaggagatgcagcagaagctggagg
acttccgcgactaccggcgtgtgcacaagccgcccaaggtgcaggagaag
tgccagctggagatcaacttcaacacgctgcagaccaagctgcgcctcag
caaccggcccgccttcatgccctccgagggcaagatggtctcggacatca
acaatggctggcagcacttggagcaggctgagaagggctacgaggagtgg
ctgctgaatgagatccgcaggctggagcggctcgaccacctggcagagaa
gttccggcagaaggcctccatccacgaggcctggactgacgggaaggaag
ccatgctgaagcaccgggactacgagacggccacactatcggacatcaaa
gccctcattcgcaagcacgaggccttcgagagcgacctggctgcgcacca
ggaccgcgtggagcagatcgccgccattgcccaggagctcaacgagctgg
attactacgactcccacaatgtcaacacccggtgccagaagatctgtgac
cagtgggacgccctcggctctctgacacatagtcgcagggaagccctgga
gaaaacagagaagcagctggaggccatcgaccagctgcacctggaatacg
ccaagcgcgcggcccccttcaacaactggatggagagcgccatggaggac
ctccaggacatgttcatcgtccataccatcgaggagattgagggcctgat
ctcagcccatgaccagttcaagtccaccctgccggacgccgatagggagc
gcgaggccatcctggccatccacaaggaggcccagaggatcgctgagagc
aaccacatcaagctgtcgggcagcaacccctacaccaccgtcaccccgca
aatcatcaactccaagtgggagaaggtgcagcagctggtgccaaaacggg
accatgccctcctggaggagcagagcaagcagcagtccaacgagcacctg
cgccgccagttcgccagccaggccaatgttgtggggccctggatccagac
caagatggaggagatcgggcgcatctccattgagatgaacgggaccctgg
aggaccagctgagccacctgaagcagtatgaacgcagcatcgtggactac
aagcccaacctggacctgctggagcagcagcaccagctcatccaggaggc
cctcatcttcgacaacaagcacaccaactataccatggagcacatccgcg
tgggctgggagcagctgctcaccaccattgcccgcaccatcaacgaggtg
gagaaccagatcctcacccgcgacgccaagggcatcagccaggagcagat
gcaggagttccgggcgtccttcaaccacttcgacaaggatcatggcgggg
cgctggggcccgaggagttcaaggcctgcctcatcagcctgggctacgac
gtggagaacgaccggcagggtgaggccgagttcaaccgcatcatgagcct
ggtcgaccccaaccatagcggccttgtgaccttccaagccttcatcgact
tcatgtcgcgggagaccaccgacacggacacggctgaccaggtcatcgct
tccttcaaggtcttagcaggggacaagaacttcatcacagctgaggagct
gcggagagagctgccccccgaccaggccgagtactgcatcgcccgcatgg
cgccataccagggccctgacgccgtgcccggtgccctcgactacaagtcc
ttctccacggccttgtatggcgagagcgacctgtgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFMVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQE
DDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLM
LLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIV
DGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNV
QNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYL
DIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVL
AVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDF
RDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINN
GWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAM
LKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDY
YDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAK
RAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADRERE
AILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDH
ALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLED
QLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVG
WEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGAL
GPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFM
SRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAP
YQGPDAVPGALDYKSFSTALYGESDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5854
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 106582 Daltons