cDNA Clone HIF1A

• Plasmid: pGEX6P-1-HIF1A
• Parent Plasmid: pGEX6P1
• Expressed: GST-HIF1A
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTC
TGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTA
AAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACAT
AATGTGAGTTCGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAG
CTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATATTGAAG
ATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTGGATGGT
TTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAA
TGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACTGGACACAGTG
TGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTT
ACACACAGAAATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACACAGCG
AAGCTTTTTTCTCAGAATGAAGTGTACCCTAACTAGCCGAGGAAGAACTA
TGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCACAGGCCACATT
CACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACC
ACCTATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCTCACCCATCAA
ATATTGAAATTCCTTTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTG
GATATGAAATTTTCTTATTGTGATGAAAGAATTACCGAATTGATGGGATA
TGAGCCAGAAGAACTTTTAGGCCGCTCAATTTATGAATATTATCATGCTT
TGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACTAAAGGA
CAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGT
CTGGGTTGAAACTCAAGCAACTGTCATATATAACACCAAGAATTCTCAAC
CACAGTGCATTGTATGTGTGAATTACGTTGTGAGTGGTATTATTCAGCAC
GACTTGATTTTCTCCCTTCAACAAACAGAATGTGTCCTTAAACCGGTTGA
ATCTTCAGATATGAAAATGACTCAGCTATTCACCAAAGTTGAATCAGAAG
ATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCTTTAACT
TTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAG
CAACGACACAGAAACTGATGACCAGCAACTTGAGGAAGTACCATTATATA
ATGATGTAATGCTCCCCTCACCCAACGAAAAATTACAGAATATAAATTTG
GCAATGTCTCCATTACCCACCGCTGAAACGCCAAAGCCACTTCGAAGTAG
TGCTGACCCTGCACTCAATCAAGAAGTTGCATTAAAATTAGAACCAAATC
CAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCAGGATCAGACA
CCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAG
TCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCA
AGTTGGAATTGGTAGAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAAC
CCATTTTCTACTCAGGACACAGATTTAGACTTGGAGATGTTAGCTCCCTA
TATCCCAATGGATGATGACTTCCAGTTACGTTCCTTCGATCAGTTGTCAC
CATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAAAGCACA
GTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCAC
CACTACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGACCGTA
TGGAAGACATTAAAATATTGATTGCATCTCCATCTCCTACCCACATACAT
AAAGAAACTACTAGTGCCACATCATCACCATATAGAGATACTCAAAGTCG
GACAGCCTCACCAAACAGAGCAGGAAAAGGAGTCATAGAACAGACAGAAA
AATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGTCAAAGA
ACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAA
TGCTCAGAGAAAGCGAAAAATGGAACATGATGGTTCACTTTTTCAAGCAG
TAGGAATTGGAACATTATTACAGCAGCCAGACGATCATGCAGCTACTACA
TCACTTTCTTGGAAACGTGTAAAAGGATGCAAATCTAGTGAACAGAATGG
AATGGAGCAAAAGACAATTATTTTAATACCCTCTGATTTAGCATGTAGAC
TGCTGGGGCAATCAATGGATGAAAGTGGATTACCACAGCTGACCAGTTAT
GATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACCTACTGCAGGG
TGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEGAGGANDKKKISSERRK
EKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRV
RKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKY
MGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFL
RMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTC
LVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEE
LLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVET
QATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDM
KMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTE
TDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPA
LNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEY
CFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMD
DDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTA
TTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASP
NRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRK
RKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQK
TIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELL
RALDQVN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU21755
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No