cDNA Clone USP6

• Plasmid: pET156P-1-USP6 cat domain 529-1406
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-USP6 cat domain
• Genbank: 9098
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCAAGGGAGCCACAGGTCTAAGCAACCTGGGAAACACATGCTTCAT
GAACTCAAGCATCCAGTGCGTTAGTAACACACAGCCACTGACACAGTATT
TTATCTCAGGGAGACATCTTTATGAACTCAACAGGACAAATCCCATTGGT
ATGAAGGGGCATATGGCTAAATGCTATGGTGATTTAGTGCAGGAACTCTG
GAGTGGAACTCAGAAGAGTGTTGCCCCATTAAAGCTTCGGCGGACCATAG
CAAAATATGCTCCCAAGTTTGATGGGTTTCAGCAACAAGACTCCCAAGAA
CTTCTGGCTTTTCTCTTGGATGGTCTTCATGAAGATCTCAACCGAGTCCA
TGAAAAGCCATATGTGGAACTGAAGGACAGTGATGGCCGACCAGACTGGG
AAGTAGCTGCAGAGGCCTGGGACAACCATCTAAGAAGAAATAGATCAATA
ATTGTGGATTTGTTCCATGGGCAGCTAAGATCTCAAGTCAAATGCAAGAC
ATGTGGGCATATAAGTGTCCGATTTGATCCTTTCAATTTTTTGTCTTTGC
CACTACCAATGGACAGTTACATGGACTTAGAAATAACAGTGATTAAGTTA
GATGGTACTACCCCTGTACGGTATGGACTAAGACTGAATATGGATGAAAA
GTACACAGGTTTAAAAAAACAGCTGAGGGATCTCTGTGGACTTAATTCAG
AACAAATCCTACTAGCAGAAGTACATGATTCCAACATAAAGAACTTTCCT
CAGGATAACCAAAAAGTACAACTCTCAGTGAGCGGATTTTTGTGTGCATT
TGAAATTCCTGTCCCTTCATCTCCAATTTCAGCTTCTAGTCCAACACAAA
TAGATTTCTCCTCTTCACCATCTACAAATGGAATGTTCACCCTAACTACC
AATGGGGACCTACCCAAACCAATATTCATCCCCAATGGAATGCCAAACAC
TGTTGTGCCATGTGGAACTGAGAAGAACTTCACAAATGGAATGGTTAATG
GTCACATGCCATCTCTTCCTGACAGCCCCTTTACAGGTTACATCATTGCA
GTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCACCTCAGGA
GAATCGCCCCAGCCTCTTTGGAATGCCATTGATTGTTCCATGCACTGTGC
ATACCCGGAAGAAAGACCTATATGATGCGGTTTGGATTCAAGTATCCTGG
TTAGCAAGACCACTCCCACCTCAGGAAGCTAGTATTCATGCCCAGGATCG
TGATAACTGTATGGGCTATCAATATCCATTCACTCTACGAGTTGTGCAGA
AAGATGGGAACTCCTGTGCTTGGTGCCCACAGTATAGATTTTGCAGAGGC
TGTAAAATTGATTGTGGGGAAGACAGAGCTTTCATTGGAAATGCCTATAT
TGCTGTGGATTGGCACCCCACAGCCCTTCACCTTCGCTATCAAACATCCC
AGGAAAGGGTTGTAGATAAGCATGAGAGTGTGGAGCAGAGTCGGCGAGCG
CAAGCCGAGCCCATCAACCTGGACAGCTGTCTCCGTGCTTTCACCAGTGA
GGAAGAGCTAGGGGAAAGTGAGATGTACTACTGTTCCAAGTGTAAGACCC
ACTGCTTAGCAACAAAGAAGCTGGATCTCTGGAGGCTTCCACCCTTCCTG
ATTATTCACCTTAAGCGATTTCAATTTGTAAATGATCAGTGGATAAAATC
ACAGAAAATTGTCAGATTTCTTCGGGAAAGTTTTGATCCGAGTGCTTTTT
TGGTACCACGAGACCCGGCCCTCTGCCAGCATAAACCACTCACACCCCAG
GGGGATGAGCTCTCCAAGCCCAGGATTCTGGCAAGAGAGGTGAAGAAAGT
GGATGCGCAGAGTTCGGCTGGAAAAGAGGACATGCTCCTAAGCAAAAGCC
CATCCTCACTCAGCGCTAACATCAGCAGCAGCCCAAAAGGTTCTCCTTCT
TCATCAAGAAAAAGTGGAACCAGCTGTCCCTCCAGCAAAAACAGCAGCCC
TAATAGCAGCCCACGGACTTTGGGGAGGAGCAAAGGGAGGCTCCGGCTGC
CCCAGATTGGCAGCAAAAATAAGCCGTCAAGTAGTAAGAAGAACTTGGAT
GCCAGCAAAGAGAATGGGGCTGGGCAGATCTGTGAGCTGGCTGACGCCTT
GAGCCGAGGGCATATGCGGGGGGGCAGCCAACCAGAGCTGGTCACTCCTC
AGGACCATGAGGTAGCTTTGGCCAATGGATTCCTTTATGAGCATGAAGCA
TGTGGCAATGGCTGTGGCGATGGCTACAGCAATGGTCAGCTTGGAAACCA
CAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGACACTCATATTAAGC
CTATTTATAATCTATATGCAATTTCATGCCATTCAGGAATTCTGAGTGGG
GGCCATTACATCACTTATGCCAAAAACCCAAACTGCAAGTGGTACTGTTA
TAATGACAGCAGCTGTGAGGAACTTCACCCTGATGAAATTGACACCGACT
CTGCCTACATTCTTTTCTATGAGCAGCAGGGGATAGACTACGCACAATTT
CTGCCAAAGATTGATGGCAAAAAGATGGCAGACACAAGCAGTACGGATGA
AGACTCTGAGTCTGATTACGAAAAGTACTCTATGTTACAGTAAGCGGCCG
C

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSKGATGLSNLGNTCFMNSSIQCVSNTQP
LTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKSVAPLKL
RRTIAKYAPKFDGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDG
RPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHISVRFDPFN
FLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLRDLC
GLNSEQILLAEVHDSNIKNFPQDNQKVQLSVSGFLCAFEIPVPSSPISAS
SPTQIDFSSSPSTNGMFTLTTNGDLPKPIFIPNGMPNTVVPCGTEKNFTN
GMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSPQENRPSLFGMPLIV
PCTVHTRKKDLYDAVWIQVSWLARPLPPQEASIHAQDRDNCMGYQYPFTL
RVVQKDGNSCAWCPQYRFCRGCKIDCGEDRAFIGNAYIAVDWHPTALHLR
YQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELGESEMYYCS
KCKTHCLATKKLDLWRLPPFLIIHLKRFQFVNDQWIKSQKIVRFLRESFD
PSAFLVPRDPALCQHKPLTPQGDELSKPRILAREVKKVDAQSSAGKEDML
LSKSPSSLSANISSSPKGSPSSSRKSGTSCPSSKNSSPNSSPRTLGRSKG
RLRLPQIGSKNKPSSSKKNLDASKENGAGQICELADALSRGHMRGGSQPE
LVTPQDHEVALANGFLYEHEACGNGCGDGYSNGQLGNHSEEDSTDDQRED
THIKPIYNLYAISCHSGILSGGHYITYAKNPNCKWYCYNDSSCEELHPDE
IDTDSAYILFYEQQGIDYAQFLPKIDGKKMADTSSTDEDSESDYEKYSML
Q*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20979
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express USP6 cat domain with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 100917 Daltons