cDNA Clone JOSD2

• Plasmid: pET156P-1-JOSD2
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-JOSD2
• Genbank: NM_138334.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCCCAGGCCCCGGGAGCACAGCCGAGCCCACCCACCGTGTA
CCACGAACGGCAGCGCCTGGAGCTGTGTGCTGTCCACGCCCTCAACAACG
TTCTGCAGCAGCAGCTCTTTAGCCAGGAGGCTGCCGATGAGATCTGCAAG
AGGTTGGCCCCAGACTCCCGGCTGAACCCTCATCGCAGCCTCCTGGGCAC
CGGCAACTATGATGTCAATGTGATCATGGCCGCTCTGCAGGGGCTGGGCC
TGGCCGCCGTGTGGTGGGACAGGAGGAGGCCCCTGTCCCAGCTGGCCCTG
CCCCAGGTACTGGGGCTGATCCTGAACCTGCCCTCGCCCGTGTCGCTGGG
GCTGCTGTCACTGCCGCTGCGCCGGCGGCACTGGGTGGCCCTGCGCCAGG
TGGACGGTGTCTACTACAACCTGGACTCCAAGCTGCGGGCGCCCGAGGCC
CTGGGGGATGAGGACGGAGTCAGGGCCTTCCTGGCGGCTGCGCTGGCCCA
GGGCCTGTGCGAGGTGCTGCTGGTAGTGACCAAGGAGGTGGAGGAGAAGG
GCAGCTGGCTGCGGACAGACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMSQAPGAQPSPPTVYHERQRLELCAVH
ALNNVLQQQLFSQEAADEICKRLAPDSRLNPHRSLLGTGNYDVNVIMAAL
QGLGLAAVWWDRRRPLSQLALPQVLGLILNLPSPVSLGLLSLPLRRRHWV
ALRQVDGVYYNLDSKLRAPEALGDEDGVRAFLAAALAQGLCEVLLVVTKE
VEEKGSWLRTD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20941
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express JOSD2 with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 23201 Daltons