cDNA Clone APP BP1

• Synonyms: NAE1, NEDD8-activating enzyme E1 regulatory subunit isoform a (APPBP1)
• Plasmid: pET28a-DAC-APP BP1
• Parent Plasmid: pET28a DAC Thr
• Expressed: DAC-APP BP1
• Genbank: NP_003896 Gene ID 8883
• Tag: DAC
• Species: Human

Sequence of Insert

AGATCTATGGCGCAGCTGGGAAAGCTGCTCAAGGAGCAGAAGTACGACCG
GCAGCTGAGGTTGTGGGGTGATCATGGGCAAGAGGCTTTAGAATCTGCTC
ATGTTTGCCTAATAAATGCAACAGCCACAGGAACTGAAATTCTTAAAAAC
TTGGTACTACCAGGTATTGGTTCGTTTACAATTATTGATGGAAATCAGGT
CAGCGGAGAAGATGCTGGAAACAATTTCTTCCTTCAAAGAAGCAGTATCG
GCAAGAACCGAGCTGAAGCTGCCATGGAATTCTTACAAGAATTAAATAGC
GATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTTCTAGACAA
TGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGTTGCAACTCAGCTTC
CTGAAAGCACTTCACTACGCTTAGCAGATGTCCTCTGGAATTCCCAGATT
CCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTTATATGAGGATCAT
TATAAAAGAACATCCAGTAATAGAATCTCATCCAGATAATGCATTAGAGG
ATCTACGACTAGATAAGCCATTTCCTGAACTGAGAGAACATTTTCAGTCC
TATGATTTGGATCATATGGAAAAAAAGGACCACAGTCATACTCCATGGAT
TGTGATCATAGCTAAATATTTAGCACAGTGGTATAGTGAAACAAATGGAC
GAATACCTAAAACGTATAAAGAAAAAGAGGACTTCAGAGATTTGATTAGA
CAAGGAATTCTAAAAAATGAAAATGGGGCTCCAGAAGATGAAGAGAATTT
TGAAGAAGCTATTAAAAATGTGAACACAGCACTAAATACAACTCAGATCC
CAAGCAGTATTGAAGATATATTTAATGATGATCGCTGCATAAATATCACC
AAACAGACTCCATCATTTTGGATTTTAGCTCGTGCCTTAAAGGAATTTGT
GGCCAAAGAGGGTCAAGGAAATTTACCTGTTCGAGGCACAATTCCTGATA
TGATTGCAGATTCAGGCAAATATATAAAACTGCAAAACGTTTACCGTGAA
AAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCATGTTGCCAAATTGCT
GCAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAATTAAAAT
TACTCTGCAGCAATTCTGCATTTCTTCGAGTGGTAAGATGTCGATCCTTA
GCTGAAGAATATGGTTTGGATACAATTAACAAGGATGAAATTATTTCTAG
CATGGACAATCCAGATAATGAAATAGTGTTGTACTTAATGTTACGGGCTG
TTGATAGATTTCATAAACAACAGGGTAGATATCCAGGAGTATCTAACTAT
CAAGTTGAAGAAGATATAGGAAAGTTGAAGTCTTGTCTCACTGGCTTCCT
TCAGGAATATGGTTTATCTGTAATGGTGAAAGATGATTATGTCCACGAAT
TTTGCCGATATGGAGCTGCTGAGCCACATACCATTGCTGCATTCTTGGGG
GGAGCTGCTGCTCAAGAGGTCATCAAAATAATCACCAAACAATTTGTAAT
TTTTAATAATACTTACATTTACAGTGGCATGTCACAAACTTCAGCAACTT
TCCAGTTGTAGGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGSMAQLGKLLKEQKYDRQLRLWGDHGQEALE
SAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRS
SIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVAT
QLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNA
LEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSET
NGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTT
QIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTI
PDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKE
LKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLML
RAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYV
HEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTS
ATFQL*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20914
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 20568, subsequently found to have nt g94a mutation in DAC tag. vector superseded by 21714 for DAC-Thr fusions