cDNA Clone HOIP

• Plasmid: pGEX6P2-HOIP delta 577-612
• Parent Plasmid: pGEX6P2
• Expressed: GST-C3-HOIP delta 577-612
• Genbank: NM_017999
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCG
CGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCC
TGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGC
TACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGA
GCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGA
AATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGT
GTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGG
GGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATG
GGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCT
ACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATT
GCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACA
AGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGA
GAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCC
TGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCAT
CCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACAC
CCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGG
TACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGT
CGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCT
GCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGA
GCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGG
GGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCCTAGAACCT
GATCTTGCACGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGA
GGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGC
CTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCC
CTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTA
CTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTA
TGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCC
TATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACG
CCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCC
AAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAA
GGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTA
CTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACG
TCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGT
GCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAA
CCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCAGCGCCAACGCC
TAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCCCT
TCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTTTTGGCAGT
CTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTCACTGCTGC
AGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAGCTGTGAGG
CACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAGGAGTGTGC
CGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCTGACTTCCT
GTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCACCATCGCC
TTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCC
CGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTACCCTTGACA
TCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGTTCCATAAG
AAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTGTGGTGTGC
CCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGAGGCAACTT
GTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAG
GAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGGAAACGCAT
GAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCTTCAGGAAA
ACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGGCCCGAGGA
GGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTCTGCAGCGG
CTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCCTAACTGCA
GGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAGACTGCCTCTTCTAC
CTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAGGACAATAA
CGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGTCCCTGGAG
GCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGCTCAGGGAC
GAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTGTGCCAGGC
ACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTCGCTGGACC
CAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTGAGCGCTAC
CTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCTGCTTACCA
GGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGGACAGAGTA
TCCCCCGCAGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMPGEEEER
AFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVR
CNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRST
VDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRT
ELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPS
VPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTL
PGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAA
CAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQS
CTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASA
QSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPK
PGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEI
FSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAW
LDRHGNLDEAVEECVRTRQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLV
RRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRL
LAQECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVC
PACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDP
KFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDF
QNWKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCR
HQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQK
LLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGY
AGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGE
DPPAYQARLLQKLTEEVPLGQSIPRRRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20906
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid for GST-HOIP delta aa577-612; ; Estimated MW of expressed protein : 143520 Daltons