cDNA Clone Lag2

• Plasmid: pGR197 Lag2 Pr.+StrepII-HA Lag2
• Parent Plasmid: pSC-B
• Expressed: StrepII-HA-Lag2
• Tag: HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

ACCGGTTACCGTATGTGTGTGTGTATTTATTTATGTAGGTTGCTAATGCT
TTGGTGATCGTTTAAAGGAACTTGGACGCAACCCTCCCACGGGTGCCAGG
GCTTGAAATCTCTTTCAATGCTGCTATTTTGCGCGACTCTGGGTACACCT
TATACCCCTTGTTTCTATGGTTAAACCAATTGAATCTCCAGCAGAGCTCG
TGAGCAAATAGCTCGCCGAAGCCGAGCATCATGCCGTTGAGGAAGGGCAA
AAGTAGGTTGATGGAGCACGAGCCTACAAAGGATACAAGGCTCTGCACAA
GTGGGCTCTCGTCGGCACTCACTGTGTTCTGCACCTCCATACAGTCTTTG
TCTTCTGATTCGTCATCTGACACGCTCTCCCAGAATCCCACAACCTCTGT
CATATTTCTGTCTTGTGGCAGTTTCGTAAGAAGGGGGGTGATGTTTCTAA
TATTACAGTGATGTTCTATTGTTCAAGTAAAGTGTATGGCTGTTTCTTAT
GGCGCGGAATATACCACTTCGTTATAAGTGATTACATCTCGTAACGCATT
CCTCTTGTTCAGTGGTAGGGGAGGCCGATGGTAGTGGAATAATACGCTTT
TATGGGCCATTGGTATTACTATTTATCTTCGTTATAATAAATAGTACACG
TTGCAGACATATTTGGAGGAGAGTTTTTAAAGTACTGTTGGGGTGCTCTT
GCTGTGCATTGTAAGTAATCGTGTCCCAGTGGCCGAGTGGTTAAGGCGAT
GCCCTGCTATTTCCTCAGAAAAGCAATTAGGCATTGGGTTTTACCTGCGC
AGGTTCGAATCCTGTCTGTGACGCATTTCTTTTTTGTGAATTCTTGCTTC
TTATCCTTCTACAATCCTTTCGTGACATTTATTGAATCATCTCTACAAAA
TTTTTACACATAGCTAAGGTTAGCTTGTTCTACATTACACAAGTGGTAGC
AAAATCGTTTACATTGCACTCAGCTATATCAACATCACGTTCTACCGCAA
CTAGTACTAACCATGTGGAGCCACCCGCAGTTCGAAAAAGGCGGAGGCATGTACCCATACGATGTGCCAGATTACGCCGCTAGC

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20894
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Intermediate for N-terminal StrepII-HA tagging of ORFS driven by Lag2 promoter (designed for use in pGR197 yeast integration vector – comprises a promoter-tag cassette)