cDNA Clone USP2b

• Plasmid: pET28-USP2b
• Parent Plasmid: pET28a original
• Expressed: 6His-USP2b
• Genbank: NM_171997.1*
• Tag: 6His

Sequence of Insert

GGATCCCCGAATTCCCGGGTCGACATGCGCACCTCGTACACCGTGACCCTGCCCGAGGACCCCCCCGCCGCCCCCTTTCCCGCCCTCGCCAAGGAGCTGCGGCCGCGCTCCCCTCTCTCCCCGTCCCTGCTGCTCTCCACCTTCGTGGGGCTCCTGCTCAACAAAGCCAAGAATTCTAAGAGTGCCCAGGGTCTGGCTGGTCTTCGAAACCTTGGGAACACGTGCTTCATGAACTCAATTCTGCAGTGCCTGAGCAACACTCGGGAGTTGAGAGATTACTGCCTCCAGAGGCTCTACATGCGGGACCTGCACCACGGCAGCAATGCACACACAGCCCTCGTGGAAGAGTTTGCAAAACTAATTCAGACCATATGGACTTCATCCCCCAATGATGTGGTGAGCCCATCTGAGTTCAAGACCCAGATCCAGAGATATGCACCGCGCTTTGTTGGCTATAATCAGCAGGATGCTCAGGAGTTCCTTCGCTTTCTTCTGGATGGGCTCCATAACGAGGTGAACCGAGTGACACTGAGACCTAAGTCCAACCCTGAGAACCTCGATCATCTTCCTGATGACGAGAAAGGCCGACAGATGTGGAGAAAATATCTAGAACGGGAAGACAGTAGGATCGGGGATCTCTTTGTTGGGCAGCTAAAGAGCTCGCTGACGTGTACAGATTGTGGTTACTGTTCTACGGTCTTCGACCCCTTCTGGGACCTCTCACTGCCCATTGCTAAGCGAGGTTATCCTGAGGTGACATTAATGGACTGCATGAGGCTCTTCACCAAAGAGGATGTGCTTGATGGAGATGAAAAGCCAACATGCTGTCGCTGCCGAGGCAGAAAACGGTGTATAAAGAAGTTCTCCATCCAGAGGTTCCCAAAGATCTTGGTGCTCCATCTGAAGCGGTTCTCAGAATCCAGGATCCGAACCAGCAAGCTCACAACATTTGTGAACTTCCCCCTAAGAGACCTGGACTTAAGAGAATTTGCCTCAGAAAACACCAACCATGCTGTTTACAACCTGTACGCTGTGTCCAATCACTCCGGAACCACCATGGGTGGCCACTATACAGCCTACTGTCGCAGTCCAGGGACAGGAGAATGGCACACTTTCAACGACTCCAGCGTCACTCCCATGTCCTCCAGCCAAGTGCGCACCAGCGACGCCTACCTGCTCTTCTACGAACTGGCCAGCCCGCCCTCCCGAATGTAGCGGCCGC

Amino Acid Sequence

HHHHHHSSGLVPRGSHMASMTGGQQMGRGSPNSRVDMRTSYTVTLPEDPP
AAPFPALAKELRPRSPLSPSLLLSTFVGLLLNKAKNSKSAQGLAGLRNLG
NTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQ
TIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEV
NRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSL
TCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEK
PTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPL
RDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTF
NDSSVTPMSSSQVRTSDAYLLFYELASPPSRM*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20875
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *1 silent nt change (c411t) compared to NCBI Acc. at Y137; Bacterial expression construct to express USP2b with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 49091 Daltons