cDNA Clone Triad1

• Synonyms: ARIH2
• Plasmid: pMal6P-1 Triad1 1-340 6His
• Parent Plasmid: pMAL6P
• Expressed: MBP-Triad1 1-3406His
• Genbank: NM_006321
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA
GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACC
CTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAG
CAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGAC
CTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTT
CTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTC
CACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA
ACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCA
CATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAG
GAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTG
GGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCT
CTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTT
CCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTT
CAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAG
ACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAG
TGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCA
CGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAG
ACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCC
AAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATG
CTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGA
CTCATGGCAGTGAATACTATGAGTGCCATCATCACCATCACCATTAGGCG
GCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMSVDMNSQGSDSNEEDYDPNCE
EEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGA
LNEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARV
QPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQFCRSCWEQHCSVL
VKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESH
YQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCAT
IRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDF
CWMCLGDWKTHGSEYYECHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20863
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express Triad aa1-340-6HIS with N-terminal MBP tag (contains preScission protease site)