cDNA Clone Den1

• Plasmid: pGEX6P-3-Den1 N91A W103A C163A
• Parent Plasmid: pGEX6P3
• Expressed: GST-Den1 N91A W103A C163A
• Genbank: NM_145204.3
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGACCCCGTAGTCTTGAGTTACATGGACAGTCTACTGCGGCA
ATCAGATGTCTCACTATTGGATCCGCCAAGCTGGCTCAATGACCATATTA
TTGGGTTTGCGTTTGAGTACTTTGCCAACAGTCAGTTTCATGACTGCTCT
GATCACGTCAGTTTCATCAGCCCTGAAGTCACCCAGTTCATCAAGTGCAC
TAGCAACCCAGCAGAGATTGCCATGTTCCTTGAACCACTGGACCTCCCCA
ACAAGAGAGTTGTATTTTTAGCCATCGCTGATAACTCCAACCAGGCAGCT
GGAGGAACCCACGCGAGTTTATTGGTCTACCTCCAAGATAAAAATAGCTT
TTTTCATTATGATTCCCATAGCAGGAGCAACTCAGTTCACGCAAAGCAGG
TAGCAGAGAAACTGGAGGCTTTCTTAGGCAGAAAAGGAGACAAACTGGCC
TTTGTGGAAGAGAAAGCCCCTGCCCAACAAAACAGCTATGACGCTGGGAT
GTACGTGATATGTAACACTGAGGCCTTGTGTCAGAACTTCTTTAGGCAAC
AGACAGAATCACTGCTGCAGCTACTCACCCCTGCATACATCACAAAGAAG
AGGGGAGAATGGAAAGATCTCATTACCACACTTGCTAAAAAGTAGGCGGC
CGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMDPVVLSYMDSLL
RQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIK
CTSNPAEIAMFLEPLDLPNKRVVFLAIADNSNQAAGGTHASLLVYLQDKN
SFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDA
GMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20825
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- Den1 N91A W103A C163A; ; ; Estimated MW of expressed protein : 51410 Daltons