cDNA Clone Den1

• Plasmid: pGEX6P-3-Den1 (delta 93-99) C163A
• Parent Plasmid: pGEX6P3
• Expressed: GST-Den1 (delta 93-99) C163A
• Genbank: NM_145204.3
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGACCCCGTAGTCTTGAGTTACATGGACAGTCTACTGCGGCA
ATCAGATGTCTCACTATTGGATCCGCCAAGCTGGCTCAATGACCATATTA
TTGGGTTTGCGTTTGAGTACTTTGCCAACAGTCAGTTTCATGACTGCTCT
GATCACGTCAGTTTCATCAGCCCTGAAGTCACCCAGTTCATCAAGTGCAC
TAGCAACCCAGCAGAGATTGCCATGTTCCTTGAACCACTGGACCTCCCCA
ACAAGAGAGTTGTATTTTTAGCCATCAATGATGGAACCCACTGGAGTTTA
TTGGTCTACCTCCAAGATAAAAATAGCTTTTTTCATTATGATTCCCATAG
CAGGAGCAACTCAGTTCACGCAAAGCAGGTAGCAGAGAAACTGGAGGCTT
TCTTAGGCAGAAAAGGAGACAAACTGGCCTTTGTGGAAGAGAAAGCCCCT
GCCCAACAAAACAGCTATGACGCTGGGATGTACGTGATATGTAACACTGA
GGCCTTGTGTCAGAACTTCTTTAGGCAACAGACAGAATCACTGCTGCAGC
TACTCACCCCTGCATACATCACAAAGAAGAGGGGAGAATGGAAAGATCTC
ATTACCACACTTGCTAAAAAGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMDPVVLSYMDSLL
RQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIK
CTSNPAEIAMFLEPLDLPNKRVVFLAINDGTHWSLLVYLQDKNSFFHYDS
HSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDAGMYVICN
TEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20824
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- Den1 delta aa93-99 C163A; ; Estimated Mw of expressed protein : 50926 Daltons