cDNA Clone RNF4

• Plasmid: pLou3-RNF4-GSGSG-Ring
• Parent Plasmid: pLOU3
• Expressed: 6His-MBP-RNF4-GSGSG-Ring
• Genbank: NP_062055.1
• Tag: MBP
• Species: Rat

Sequence of Insert

CCATGGATATGAGTACAAGAAATCCTCAAAGAAAGCGGCGTGGTGGAGCTGTGAATTCTAGACAAACCCAGAAGCGAACTCGGGAAACAACTTCAACCCCTGAGATTTCCTTGGAAGCAGAACCCATAGAACTTGTGGAAACCGTTGGAGATGAAATCGTGGACCTCACCTGTGAATCTTTAGAGCCTGTGGTTGTGGACCTGACTCACAATGACTCTGTTGTGATTGTTGAAGAAAGGAGAAGGCCAAGGAGAAATGGGAGGAGGTTGCGCCAAGACCATGCTGACAGCTGTGTGGTGAGCAGTGATGATGAAGAACTGTCTAAGGACAAAGATGTATACGTGACTACCCATACTCCTCGAAGCACCAAGGATGAAGGAACTACTGGACTTAGGCCCTCGGGCACCGTCAGTTGTCCGATCTGCATGGATGGATACTCTGAGATTGTGCAGAATGGGCGTCTCATTGTTTCTACAGAATGTGGCCACGTCTTCTGTAGCCAGTGCCTCCGTGATTCCCTTAAGAATGCTAACACTTGCCCAACTTGTAGGAAAAAGATCAACCATAAACGGTACCATCCCATTTATATAGGATCCGGTTCTGGTTCGGGCACCGTCAGTTGTCCGATCTGCATGGATGGATACTCTGAGATTGTGCAGAATGGGCGTCTCATTGTTTCTACAGAATGTGGCCACGTCTTCTGTAGCCAGTGCCTCCGTGATTCCCTTAAGAATGCTAACACTTGCCCAACTTGTAGGAAAAAGATCAACCATAAACGGTACCATCCCATTTATATATGATAAAAGCTT

Amino Acid Sequence

MHHHHHHHMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLYFQGAMDMSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYIGSGSGSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pLou3-RNF4-GSGSG-Ring114.15 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20803
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: construct for bacterial expression of N-terminally 6HIS.MBP tagged full-length RNF4 (Rattus norvegicus) fused to the RNF4 RING domain (residues 131-194), separated by a Gly-Ser-Gly-Ser-Gly linker in pLou3 vector (49251). Full-length RNF4 flanked NcoI-BamHI, RING domain flanked BamHI-HindIII. 6His-MBP cleavable with TEV
• Low Temp: No