cDNA Clone ISG15

• Plasmid: pTYB-ISG15-Intein-6His
• Parent Plasmid: pTYB
• Expressed: ISG15-Intein-6His
• Genbank: NP_005092
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGGGCTGGGACCTGACGGTGAAGATGCTGGCGGGCAACGAATTCCA
GGTGTCCCTGAGCAGCTCCATGTCGGTGTCAGAGCTGAAGGCGCAGATCA
CCCAGAAGATCGGCGTGCACGCCTTCCAGCAGCGTCTGGCTGTCCACCCG
AGCGGTGTGGCGCTGCAGGACAGGGTCCCCCTTGCCAGCCAGGGCCTGGG
CCCCGGCAGCACGGTCCTGCTGGTGGTGGACAAATGCGACGAACCTCTGA
ACATCCTGGTGAGGAATAACAAGGGCCGCAGCAGCACCTACGAGGTGCGG
CTGACGCAGACCGTGGCCCACCTGAAGCAGCAAGTGAGCGGGCTGGAGGG
TGTGCAGGACGACCTGTTCTGGCTGACCTTCGAGGGGAAGCCCCTGGAGG
ACCAGCTCCCGCTGGGGGAGTACGGCCTCAAGCCCCTGAGCACCGTGTTC
ATGAATCTGCGCCTGCGGGGAGGCTGCTTTGCCAAGGGTACC

Amino Acid Sequence

MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHP
SGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLNILVRNNKGRSSTYEVR
LTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVF
MNLRLRGGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPR
GRETMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRL
SRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANEL
VESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDY
MQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEY
AEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVG
LGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTI
HTSVRDGLVSLARSLGLVVSVNAEPAKVDMNVTKHKISYAIYMSGGDVLL
NVLSKCAGSKKFRPAPAAAFARECRGFYFELQELKEDDYYGITLSDDSDH
QFLLGSQVVVHACGGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKT
YKCLQPHTSLAGWEPSNVPALWQLQHHHHHH*

• Antibiotic: Amp
• Full Snapgene Map: pTYB-ISG15-Intein-6His834.96 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20800
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression vector ISG15 (1-157)-Intein-6HIS; Parental Plasmid pYTB2 (DU32127); Estimated MW of expressed protein : 75515 Daltons
• Low Temp: No