cDNA Clone Ubiquitin

• Plasmid: pGEX-TEV-Ubiquitin (c220a mutant)
• Parent Plasmid: pGEX TEV
• Expressed: GST-Ubiquitin (c220a mutant)
• Genbank: P62988*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGGTATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSGMQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20776
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Differs from reference sequence in having silent mutation at Arg73; Bacterial expression vector with N-terminal GST tag and TEV protease cleavage site. ; ; Estimated MW of expressed protein : 35301 Daltons; Parental: 20757