cDNA Clone Fan1

• Plasmid: pGEX6P1-Fan1 UBZ domain tetramer (+ start and stop)
• Parent Plasmid: pGEX6P1
• Expressed: GST-Fan1 UBZ domain tetramer
• Genbank: NM_014967.3 GI:62198230
• Tag: GST
• Species: Human

Sequence of Insert

GCCGGCAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCGGTGGAGGTGGAGGTGGAGGCGGCAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCCTAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACTTCGGTGGAGGTGGAGGTGGAGGCGCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGKLACPVCSKMVPRYDL
NRHLDEMCANNDFGGGGGGGGKLACPVCSKMVPRYDLNRHLDEMCANNDF
GGGGGGGGKLACPVCSKMVPRYDLNRHLDEMCANNDFGGGGGGGGKLACP
VCSKMVPRYDLNRHLDEMCANNDF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20765
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-Fan1 UBZ domain tetramer; Estimated MW of expressed protein : 41935 Daltons; All MTMR15 (FAN1) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Parental SC20727 (pGEX6P1-Fan1 UBZ domain dimer)