cDNA Clone Lag2

• Plasmid: pYES2-GFP-Lag2
• Parent Plasmid: pSC-B
• Expressed: GFP-Lag2
• Genbank: NM_001183279.1
• Tag: GFP
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCGGAGGTGGAGGTGGAGGTGGAGGTACCATGAGCTTGCACATTAG
TAAACTGATTGAGCAATATCGTTCCACTAAGGACAATGACCTTAAATATA
TGCTTTTAAGGCAAAACTTTAAAATTAACGACATAGAGGATGAGCTGGCT
CCTCTTGTTAACGAATTGCTGCTCCCGGTATTGGTCGAAGAACAAGACAT
GGAAATTCTCAACCTAGTATCGTTTCAAGTGCTCCCCGATTTAGTTCTTT
CTATGATCAGCGACCCTGCAGCTGCTCAGTTGGGATGGGTAATAAGCTTG
ATATGCGACCCTTTATTAAACCAAAGCATGATACATGCAAATCGTTCTTT
TGTTCTGATTGAGACACTAAGAAATGTGTTACAAAAAATTGAAAACTCCC
CTCATTTAGATTACCACCAACCAGTAAACAGTTCATTAGAGTTTATCTCC
AAATTTATTGTTGAAATGAAAAGGCATATGTGTGATGTGGATGCTGCGCA
GCTTTCACATTCACTTTCTGAAAGCAATATGCTAATTTACATAGAATCCC
TTAATCTATTGCTAAAATTTAGCTTCTTTTCAGACGCGGCAAGTCCTTCA
GTAATGGTCACACTGCCCTTTGATATTTTGAATGACGTTTTTACAATTGC
GCAGGACTATTCTGCTACGAATACAAATGAAAGCATTGATAGGATCACAG
AAAAATTACTTTTGACTTCTACGCAATTAACTCATCCGGTGGACCTGGAA
AATTTATGCCCCAAAATGAAGTACAATACTTTAGCAGCAGTTTCTCGAAT
ATGGTACAAATTTGGCCCCATAGTCGATAAATTATTTACTAACCGGCTAT
TACCGGTTCTATTCCCCCCGCAAATGGGAGAAGAATGTAACGTCGAAGAT
GTTCTCGAAATAGTTCATAACTTCCATCCATATTTTTCTATTCGTAGGCT
AAAGGATAATAGGCCTCTATTGTCAGATTCGACTATTAGCCAGCTAAGAG
AGGGTTTGTTTGGTATGCTCAGCATATTAAACGACTCGCTGACAAGGACG
CAAAATGAAAACGACCATGGTAGCGACAATCTGATCGACAGTGACGACGG
ATTTGGCTCAGACAACGATCCTGAACAACAAGCGTACCTCGATGAACTCG
TATCAGAAGGTTATGACGAAAACATGTATGACGGTGATACGGATGACGAA
GACGCAGATGATATAAACGTTGAAAAAAATGATGAAGCCACCAAAGACAT
TACAGAAACGAACAAAATCCTTTTAATCTTTTCTGAGTTACATTATCCTC
AGGAGGAGCGCTTCTCTGAACTGTTGGTTGAGTTGCAGACTAAGATCGCT
ATAAACACATCCTTAATTGATAAAATTCTTTCAAAAGAGACAACAGAATT
ACCCACTCACAATGGGGAAATCGCAGATTTGAACGAAATTCTAAATGAAG
TGAAAGGCAATAAACCAATTAGAAAAAACGTTATTTTTTGTACGTTAGCG
CATACTTTGTCGTTGCAATCCGGTTCCGAATTGTCTGTTCTACAGCTATC
AATAGAGGTTATTGATCATCTGCTGGTTAAAAATCATTCAAATAACATCA
CTCGTGGCGAGCAATTTCAATTGATTAAGTTGATATTACCCCATCTAAAA
ACAAATAAATCTTTTATTGATACATTAAAAGCAGGAAATTTTACCCAAAA
AATTGATGAAGGAGTGACCTTAAGAACAATGATTCTTTCTCTACTACTAC
AACTATTCCCACTGGATTACTCTATGCTAGGTGAGATTTTACCCACTATT
GCGCGATATTCTGTAAGGGATAAGGATCTTGGGGTGAGAGATCTGTCCTT
CCAATTGCTGGACCAAATTCTACGCACATACTATAATTACCTAATTGGTA
TCGATTGGGAATGGTATAAAGATGACTTCTATCAAGTTTTGCAAGAAACT
TGTATTAAAAAAGACATAAATACCAACCTCTTGCTACAATTTCCTCCTTA
TCTCCCTCATGACTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGGGGGGGGT
MSLHISKLIEQYRSTKDNDLKYMLLRQNFKINDIEDELAPLVNELLLPVL
VEEQDMEILNLVSFQVLPDLVLSMISDPAAAQLGWVISLICDPLLNQSMI
HANRSFVLIETLRNVLQKIENSPHLDYHQPVNSSLEFISKFIVEMKRHMC
DVDAAQLSHSLSESNMLIYIESLNLLLKFSFFSDAASPSVMVTLPFDILN
DVFTIAQDYSATNTNESIDRITEKLLLTSTQLTHPVDLENLCPKMKYNTL
AAVSRIWYKFGPIVDKLFTNRLLPVLFPPQMGEECNVEDVLEIVHNFHPY
FSIRRLKDNRPLLSDSTISQLREGLFGMLSILNDSLTRTQNENDHGSDNL
IDSDDGFGSDNDPEQQAYLDELVSEGYDENMYDGDTDDEDADDINVEKND
EATKDITETNKILLIFSELHYPQEERFSELLVELQTKIAINTSLIDKILS
KETTELPTHNGEIADLNEILNEVKGNKPIRKNVIFCTLAHTLSLQSGSEL
SVLQLSIEVIDHLLVKNHSNNITRGEQFQLIKLILPHLKTNKSFIDTLKA
GNFTQKIDEGVTLRTMILSLLLQLFPLDYSMLGEILPTIARYSVRDKDLG
VRDLSFQLLDQILRTYYNYLIGIDWEWYKDDFYQVLQETCIKKDINTNLL
LQFPPYLPHD*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20722
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression vector for Lag2 N-terminally tagged with GFP and an 8Gly linker; ; Estimated MW of expressed protein : 103265 Daltons