cDNA Clone Fan1

• Plasmid: pGEX6P-1-Fan1 UBZ domain monomer (+ start and stop)
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Fan1 UBZ domain
• Genbank: NM_014967.3 GI:62198230
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCCGGCAAACTTGCCTGCCCCGTTTGCAGTAAAATGGTGCC
TAGATATGACTTAAACCGGCACCTTGATGAAATGTGTGCTAACAATGACT
TCTGAGGCGCCGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGKLACPVCSKMVPRYDL
NRHLDEMCANNDF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20707
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-Fan1 UBZ domain monomer; Also acts as Intermediate for construction of UBZ domain concatamers; ; Estimated MW of expressed protein : 30468 Daltons; parental SC20103 (pGEX6P-1 minus Sfo1 site)