cDNA Clone Ras

• Plasmid: pcDNA3.1 Ras G12V
• Parent Plasmid: pcDNA3.1
• Expressed: Ras G12V
• Genbank: NM_001130442.1 P01112
• Species: Human

Sequence of Insert

GGATCCGCCACCATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGTCGG
TGTGGGCAAGAGTGCGCTGACCATCCAGCTGATCCAGAACCATTTTGTGG
ACGAATACGACCCCACTATAGAGGATTCCTACCGGAAGCAGGTGGTCATT
GATGGGGAGACGTGCCTGTTGGACATCCTGGATACCGCCGGCCAGGAGGA
GTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGT
GTGTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTAC
AGGGAGCAGATCAAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCT
GGTGGGGAACAAGTGTGACCTGGCTGCACGCACTGTGGAATCTCGGCAGG
CTCAGGACCTCGCCCGAAGCTACGGCATCCCCTACATCGAGACCTCGGCC
AAGACCCGGCAGGGAGTGGAGGATGCCTTCTACACGTTGGTGCGTGAGAT
CCGGCAGCACAAGCTGCGGAAGCTGAACCCTCCTGATGAGAGTGGCCCCG
GCTGCATGAGCTGCAAGTGTGTGCTCTCCTGAGCGGCCGC

Amino Acid Sequence

MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGET
CLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQI
KRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQ
GVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20700
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for HRas G12V; ; Estimated MW of expressed protein : 21340 Daltons