cDNA Clone Hdm2

• Plasmid: pCMV-FLAG Hdm2
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-Hdm2
• Genbank: NM_002392.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTGCAATACCAACATGTCTGTACCTACTGATGGTGCTGTAAC
CACCTCACAGATTCCAGCTTCGGAACAAGAGACCCTGGTTAGACCAAAGC
CATTGCTTTTGAAGTTATTAAAGTCTGTTGGTGCACAAAAAGACACTTAT
ACTATGAAAGAGGTTCTTTTTTATCTTGGCCAGTATATTATGACTAAACG
ATTATATGATGAGAAGCAACAACATATTGTATATTGTTCAAATGATCTTC
TAGGAGATTTGTTTGGCGTGCCAAGCTTCTCTGTGAAAGAGCACAGGAAA
ATATATACCATGATCTACAGGAACTTGGTAGTAGTCAATCAGCAGGAATC
ATCGGACTCAGGTACATCTGTGAGTGAGAACAGGTGTCACCTTGAAGGTG
GGAGTGATCAAAAGGACCTTGTACAAGAGCTTCAGGAAGAGAAACCTTCA
TCTTCACATTTGGTTTCTAGACCATCTACCTCATCTAGAAGGAGAGCAAT
TAGTGAGACAGAAGAAAATTCAGATGAATTATCTGGTGAACGACAAAGAA
AACGCCACAAATCTGATAGTATTTCCCTTTCCTTTGATGAAAGCCTGGCT
CTGTGTGTAATAAGGGAGATATGTTGTGAAAGAAGCAGTAGCAGTGAATC
TACAGGGACGCCATCGAATCCGGATCTTGATGCTGGTGTAAGTGAACATT
CAGGTGATTGGTTGGATCAGGATTCAGTTTCAGATCAGTTTAGTGTAGAA
TTTGAAGTTGAATCTCTCGACTCAGAAGATTATAGCCTTAGTGAAGAAGG
ACAAGAACTCTCAGATGAAGATGATGAGGTATATCAAGTTACTGTGTATC
AGGCAGGGGAGAGTGATACAGATTCATTTGAAGAAGATCCTGAAATTTCC
TTAGCTGACTATTGGAAATGCACTTCATGCAATGAAATGAATCCCCCCCT
TCCATCACATTGCAACAGATGTTGGGCCCTTCGTGAGAATTGGCTTCCTG
AAGATAAAGGGAAAGATAAAGGGGAAATCTCTGAGAAAGCCAAACTGGAA
AACTCAACACAAGCTGAAGAGGGCTTTGATGTTCCTGATTGTAAAAAAAC
TATAGTGAATGATTCCAGAGAGTCATGTGTTGAGGAAAATGATGATAAAA
TTACACAAGCTTCACAATCACAAGAAAGTGAAGACTATTCTCAGCCATCA
ACTTCTAGTAGCATTATTTATAGCAGCCAAGAAGATGTGAAAGAGTTTGA
AAGGGAAGAAACCCAAGACAAAGAAGAGAGTGTGGAATCTAGTTTGCCCC
TTAATGCCATTGAACCTTGTGTGATTTGTCAAGGTCGACCTAAAAATGGT
TGCATTGTCCATGGCAAAACAGGACATCTTATGGCCTGCTTTACATGTGC
AAAGAAGCTAAAGAAAAGGAATAAGCCCTGCCCAGTATGTAGACAACCAA
TTCAAATGATTGTGCTAACTTATTTCCCCTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLK
SVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVP
SFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLV
QELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSI
SLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQD
SVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTD
SFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKG
EISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQ
ESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCV
ICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTY
FP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20698
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminally FLAG tagged Hdm2 for mammalian expression; ; Estimated MW of expressed protein : 56504 Daltons