cDNA Clone UBE2J2

• Synonyms: -
• Plasmid: pET28a-UBE2J2 1-226
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2J2 1-226
• Genbank: NM_058167
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAGCAGCACCAGCAGTAAGAGGGCTCCGACCACGGCAACCCA
GAGGCTGAAGCAGGACTACCTTCGCATTAAGAAAGACCCGGTGCCTTACA
TCTGTGCCGAGCCCCTCCCTTCGAATATTCTCGAGTGGCACTATGTCGTC
CGAGGCCCAGAGATGACCCCTTATGAAGGTGGCTATTATCATGGAAAACT
AATTTTTCCCAGAGAATTTCCTTTCAAACCTCCCAGTATCTATATGATCA
CTCCCAACGGGAGGTTTAAGTGCAACACCAGGCTGTGTCTTTCTATCACG
GATTTCCACCCGGACACGTGGAACCCGGCCTGGTCTGTCTCCACCATCCT
GACTGGGCTCCTGAGCTTCATGGTGGAGAAGGGCCCCACCCTGGGCAGTA
TAGAGACGTCGGACTTCACGAAAAGACAACTGGCAGTGCAGAGTTTAGCA
TTTAATTTGAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTCGTGGA
GGAGATTAAACAAAAACAGAAAGCACAAGACGAACTCAGTAGCAGACCCC
AGACTCTCCCCTTGCCAGACGTGGTTCCAGACGGGGAGACGCACCTCGTC
CAGAACGGGATTCAGCTGCTCAACGGGCATGCGCCGGGGGCCGTCCCAAA
CCTCGCAGGGCTCCAGCAGGCCAACCGGCACCACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMSSTSSKRAPTTATQR
LKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLI
FPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILT
GLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELFPEVVEE
IKQKQKAQDELSSRPQTLPLPDVVPDGETHLVQNGIQLLNGHAPGAVPNL
AGLQQANRHH*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28a-UBE2J2 1-22645.54 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20695
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2J2 aa 1-1 226 with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 28987 Daltons
• Low Temp: No