cDNA Clone KLHL7

• Plasmid: pEFIRES-P-eYFP C1-KLHL7
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eFYP-KLHL7
• Genbank: NM_001031710
• Tag: YFP
• Species: Human

Sequence of Insert

agatctATGGCAGCCTCTGGGGTGGAGAAGAGCAGCAAGAAGAAGACCGA
GAAGAAACTTGCTGCTCGGGAAGAAGCTAAATTGTTGGCGGGTTTCATGG
GCGTCATGAATAACATGCGGAAACAGAAAACGTTGTGTGACGTGATCCTC
ATGGTCCAGGAAAGAAAGATACCTGCTCATCGTGTTGTTCTTGCTGCAGC
CAGTCATTTTTTTAACTTAATGTTCACAACTAACATGCTTGAATCAAAGT
CCTTTGAAGTAGAACTCAAAGATGCTGAACCTGATATTATTGAACAACTG
GTGGAATTTGCTTATACTGCTAGAATTTCCGTGAATAGCAACAATGTTCA
GTCTTTGTTGGATGCAGCAAACCAATATCAGATTGAACCTGTGAAGAAAA
TGTGTGTTGATTTTTTGAAAGAACAAGTTGATGCTTCAAATTGTCTTGGT
ATAAGTGTGCTAGCGGAGTGTCTAGATTGTCCTGAATTGAAAGCAACTGC
AGATGACTTTATTCATCAGCACTTTACTGAAGTTTACAAAACTGATGAAT
TTCTTCAACTTGATGTCAAGCGAGTAACACATCTTCTCAACCAGGACACT
CTGACTGTGAGAGCAGAGGATCAGGTTTATGATGCTGCAGTCAGGTGGTT
GAAATACGATGAACCTAATCGCCAGCCATTTATGGTTGATATCCTTGCTA
AAGTCAGGTTTCCTCTTATATCAAAGAATTTCTTAAGTAAAACGGTACAA
GCTGAACCACTTATTCAAGACAATCCTGAATGCCTTAAGATGGTGATAAG
TGGAATGAGGTACCATCTACTGTCTCCAGAGGACCGAGAAGAACTTGTAG
ATGGCACAAGACCTAGAAGAAAGAAACATGACTACCGCATAGCCCTATTT
GGAGGCTCTCAACCACAGTCTTGTAGATATTTTAACCCAAAGGATTATAG
CTGGACAGACATCCGCTGCCCCTTTGAAAAACGAAGAGATGCAGCATGCG
TGTTTTGGGACAATGTAGTATACATTTTGGGAGGCTCTCAGCTTTTCCCA
ATAAAGCGAATGGACTGCTATAATGTAGTGAAGGATAGCTGGTATTCGAA
ACTGGGTCCTCCGACACCTCGAGACAGCCTTGCTGCATGTGCTGCAGAAG
GCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCAGCTCTGTAT
TTATTTGAGTGCTATGATACGAGAACTGAAAGCTGGCACACAAAGCCCAG
CATGCTGACCCAGCGCTGCAGCCATGGGATGGTGGAAGCCAATGGCCTAA
TCTATGTTTGTGGTGGAAGTTTAGGAAACAATGTTTCTGGGAGAGTGCTT
AATTCCTGTGAAGTTTATGATCCTGCCACAGAAACATGGACTGAGCTGTG
TCCAATGATTGAAGCCAGGAAGAATCATGGGCTGGTATTTGTAAAAGACA
AGATATTTGCTGTGGGTGGTCAGAATGGTTTAGGTGGTCTGGACAATGTG
GAATATTACGATATTAAGTTGAACGAATGGAAGATGGTCTCACCAATGCC
ATGGAAGGGTGTAACAGTGAAATGTGCAGCAGTTGGCTCTATAGTTTATG
TCTTGGCTGGTTTTCAGGGTGTTGGTCGATTAGGACACATTCTCGAATAT
AATACCGAAACAGACAAATGGGTTGCCAACTCCAAAGTTCGTGCTTTTCC
AGTCACAAGTTGTTTAATTTGTGTTGTCGATACTTGTGGAGCAAATGAAG
AGACCCTTGAAACATGAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMAASGVEKSSKKKTEKKLAAREEAKLLAGFMGVMNNMRKQKTLCDVILMVQERKIP
AHRVVLAAASHFFNLMFTTNMLESKSFEVELKDAEPDIIEQLVEFAYTARISVNSNNVQSLLDAANQYQIEPVKKMCVDFLKEQVDASNCLGISVLAECL
DCPELKATADDFIHQHFTEVYKTDEFLQLDVKRVTHLLNQDTLTVRAEDQVYDAAVRWLKYDEPNRQPFMVDILAKVRFPLISKNFLSKTVQAEPLIQDN
PECLKMVISGMRYHLLSPEDREELVDGTRPRRKKHDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYN
VVKDSWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQRCSHGMVEANGLIYVCGGSLGNNVSGRVLNSCEVYDP
ATETWTELCPMIEARKNHGLVFVKDKIFAVGGQNGLGGLDNVEYYDIKLNEWKMVSPMPWKGVTVKCAAVGSIVYVLAGFQGVGRLGHILEYNTETDKWV
ANSKVRAFPVTSCLICVVDTCGANEETLET*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20673
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: transcript variant 1. Silent change at Leu118 compared to NCBI reference