cDNA Clone maxFP Green

• Plasmid: pTRE-Tight-maxFPGreen-SV5 (+ stop)
• Parent Plasmid: pTRE-Tight
• Expressed: maxFPGreen-SV5
• Genbank: AY268072.1*
• Tag: SV5
• Species: Pontellina plumata

Sequence of Insert

GGATCCGCCACCATGGAGAGCGACGAGAGCGGCCTGCCCGCCATGGAGAT
CGAGTGCCGCATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGG
GCGGCGGAGAGGGCACCCCCGAGCAGGGCCGCATGACCAACAAGATGAAG
AGCACCAAAGGCGCCCTGACCTTCAGCCCCTACCTGCTGAGCCACGTGAT
GGGCTACGGCTTCTACCACTTCGGCACCTACCCCAGCGGCTACGAGAACC
CCTTCCTGCACGCCATCAACAACGGCGGCTACACCAACACCCGCATCGAG
AAGTACGAGGACGGCGGCGTGCTGCACGTGAGCTTCAGCTACCGCTACGA
GGCCGGCCGCGTGATCGGCGACTTCAAGGTGATGGGCACCGGCTTCCCCG
AGGACAGCGTGATCTTCACCGACAAGATCATCCGCAGCAACGCCACCGTG
GAGCACCTGCACCCCATGGGCGATAACGATCTGGATGGCAGCTTCACCCG
CACCTTCAGCCTGCGCGACGGCGGCTACTACAGCTCCGTGGTGGACAGCC
ACATGCACTTCAAGAGCGCCATCCACCCCAGCATCCTGCAGAACGGGGGC
CCCATGTTCGCCTTCCGCCGCGTGGAGGAGGATCACAGCAACACCGAGCT
GGGCATCGTGGAGTACCAGCACGCCTTCAAGACCCCGGATGCAGATGCCG
GTGAAGAAGGCAAGCCCATCCCCAACCCCCTGCTGGGCCTGGACAGCACC
TAAGCGGCCGC

Amino Acid Sequence

MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKG
ALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYED
GGVLHVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLH
PMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEEGKPIPNPLLGLDST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20643
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Differs from reference protein in having 8 residue N-terminal extension, 3 residue C-terminal extension and the following substitutions (numbers refer to reference sequence): K5E, V117M, V149D, V151D, A155T, F168S, L200D, I219D, F221D.; Mammalian response plasmid for expression of maxFPGreen-SV5 (for use with Clontech Advanced Tet-ON system); ; Estimated MW of expressed protein : 27097 Daltons