cDNA Clone USP3

• Plasmid: pET28-DAC-USP3
• Parent Plasmid: pET28a-DAC (SC20568)
• Expressed: DAC-USP3
• Tag: DAC

Sequence of Insert

GGATCCATGGAGTGTCCACACCTGAGCTCCAGCGTCTGCATTGCTCCGGACTCAGCCAAGTTCCCCAACGGCTCCCCGTCGTCCTGGTGCTGCAGCGTGTGCCGGTCCAACAAAAGCCCTTGGGTCTGTTTGACTTGTTCAAGTGTCCACTGTGGAAGGTATGTGAATGGCCATGCAAAAAAACATTATGAAGATGCACAAGTACCTTTAACCAACCATAAGAAATCAGAAAAGCAAGATAAAGTTCAGCACACAGTATGTATGGATTGCAGTAGCTACAGTACATACTGTTATCGCTGTGATGATTTTGTGGTTAATGACACCAAGCTGGGACTGGTACAGAAAGTCAGAGAACACTTACAGAACTTGGAAAACTCAGCTTTCACAGCTGACAGGCATAAGAAAAGAAAACTTTTGGAAAACTCAACACTAAACAGCAAGTTATTAAAAGTAAATGGAAGCACCACTGCCATTTGTGCCACAGGCCTTCGGAATTTGGGGAACACATGTTTCATGAATGCCATCCTTCAGTCACTCAGTAACATTGAGCAGTTTTGCTGTTATTTCAAAGAACTGCCCGCCGTGGAGTTAAGGAATGGGAAAACAGCAGGAAGGCGGACATACCACACCAGGAGCCAAGGGGATAACAATGTGTCTTTGGTAGAAGAGTTTAGAAAGACACTCTGTGCTTTATGGCAAGGCAGCCAGACTGCATTTAGCCCAGAGTCCTTATTTTATGTTGTTTGGAAGATTATGCCAAACTTTAGGGGCTATCAACAGCAGGACGCCCATGAATTCATGCGCTACCTTTTGGACCACCTACACTTGGAACTTCAGGGCGGTTTCAACGGTGTTTCCCGCTCAGCAATTCTGCAGGAGAATTCTACTCTGTCTGCAAGTAACAAGTGTTGCATAAATGGAGCATCTACTGTTGTCACGGCTATATTCGGAGGCATTCTCCAAAATGAGGTTAACTGCCTCATATGTGGGACAGAATCTAGAAAGTTTGATCCATTCCTAGACCTTTCATTAGATATTCCAAGTCAGTTCAGAAGTAAGCGCTCTAAGAATCAAGAAAATGGACCAGTTTGTTCGTTACGAGATTGTCTTCGCAGTTTTACCGACTTAGAAGAACTTGATGAGACAGAGTTATATATGTGCCATAAATGCAAAAAGAAACAAAAGTCCACAAAAAAGTTTTGGATTCAAAAACTACCCAAGGTGCTATGCTTACATTTGAAAAGATTTCATTGGACAGCATATTTAAGAAATAAAGTTGATACATACGTAGAATTTCCACTGAGAGGCCTAGACATGAAATGCTACTTACTAGAGCCTGAGAACAGTGGCCCGGAGAGCTGCCTGTATGACCTCGCCGCTGTGGTGGTGCACCATGGTTCCGGGGTTGGTTCTGGACATTACACAGCATACGCAACTCACGAAGGCCGCTGGTTCCACTTCAATGACAGTACTGTAACACTGACTGACGAGGAGACTGTGGTGAAGGCGAAGGCCTACATCCTTTTCTACGTGGAACACCAGGCCAAAGCTGGATCGGATAAACTTTAAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGSMECPHLSSSVCIAPDSAKFPNGSPSSWCC
SVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDK
VQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTAD
RHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSN
IEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCAL
WQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGG
FNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGT
ESRKFDPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDE
TELYMCHKCKKKQKSTKKFWIQKLPKVLCLHLKRFHWTAYLRNKVDTYVE
FPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHE
GRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20633
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression vector for N-terminally DAC tagged USP13 (contains thrombin cleavage site); ; Estimated MW of expressed protein : 88269 Daltons